LOCUS       Exported                6461 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    107_pAAV-ProC17-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        580..776
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      627..762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     689..708
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      783..792
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             789..1712
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1719..2435
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1764..1785)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2025..2044)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2372..2393
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2466..3054
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2519..2539)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2937..2948)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3086..3562
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3285..3304)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3602..3742
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3602..3731
                     /label=AAV2 ITR
     rep_origin      3817..4272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3904..3923)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4114..4135
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4289..4308)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4408..4430
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4468..4486)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4554..4658
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4659..5519
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4877..4896)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5690..6278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6179..6198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6340..8
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gggttcctgc ggccgcacgc gtaacaagtc tatcataata attacaggat gtgaacctgg
       61 gaggataatt acaggacccc gggcgcaata aataattaca ggaatcgaat atggaattat
      121 aattacagga tctgaagaat caagtataat tacaggagcc gttgtgtgct gtataattac
      181 aggaatgatc atttattgca taattacagg ataggtgtgc cgctacataa ttacaggagg
      241 tttcagccca actataatta caggactagg caagttcggt ataattacag gatggcgctg
      301 ccccccaata attacaggat agctgacttc ggtaataatt acaggacatt ctcgccagac
      361 tataattaca ggatgcaact agactctgat aattacagga tcgtcttaaa ttgccataat
      421 tacaggaaca agcatacgtt gcataattac aggaccccta ttctagtgta taattacagg
      481 atgtgcacaa ccaaaaataa ttacaggata attgtcttga ctgataatta caggagggtg
      541 tgatacacct ataattacag gagctcgaga tctgcgatct gcatctcaat tagtcagcaa
      601 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
      661 ctccgcccca tcgctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
      721 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaggat
      781 ccgccaccat ggattatgga ggcgccctga gtgccgttgg gcgcgagctg ctatttgtaa
      841 cgaacccagt agtcgtcaat ggctctgtac ttgtgcctga ggaccagtgt tactgcgcgg
      901 gctggattga gtcgcgtggc acaaacggtg cccaaacggc gtcgaacgtg ctgcaatggc
      961 ttgctgctgg cttctccatc ctactgctta tgttttacgc ctaccaaaca tggaagtcaa
     1021 cctgcggctg ggaggagatc tatgtgtgcg ctatcgagat ggtcaaggtg attctcgagt
     1081 tcttcttcga gtttaagaac ccgtccatgc tgtatctagc cacaggccac cgcgtccagt
     1141 ggttgcgtta cgccgagtgg cttctcacct gcccggtcat ttgcattcac ctgtcaaacc
     1201 tgacgggctt gtccaacgac tacagcaggc gcaccatggg tctgcttgtg tctgatattg
     1261 gcacaattgt gtggggcgcc acttccgcca tggccaccgg atacgtcaag gtcatcttct
     1321 tctgcctggg tctgtgttat ggtgctaaca cgttctttca cgctgccaag gcctacatcg
     1381 agggttacca caccgtgccg aagggccggt gtcgccaggt ggtgactggc atggcttggc
     1441 tcttcttcgt atcatggggt atgttcccca tcctgttcat cctcggcccc gagggcttcg
     1501 gcgtcctgag cgtgtacggc tccaccgtcg gccacaccat cattgacctg atgtcgaaga
     1561 actgctgggg tctgctcggc cactacctgc gcgtgctgat ccacgagcat atcctcatcc
     1621 acggcgacat tcgcaagacc accaaattga acattggtgg cactgagatt gaggtcgaga
     1681 cgctggtgga ggacgaggcc gaggctggcg cggtacccat ggtgagcaag ggcgaggagc
     1741 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1801 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1861 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     1921 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1981 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     2041 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     2101 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     2161 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     2221 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     2281 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     2341 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2401 ccgggatcac tctcggcatg gacgagctgt acaagtgata agaattcgat atcaagctta
     2461 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2521 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2581 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2641 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     2701 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     2761 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     2821 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     2881 tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     2941 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3001 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     3061 accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct
     3121 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
     3181 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg
     3241 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag
     3301 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat
     3361 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta
     3421 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc
     3481 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac
     3541 cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg
     3601 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     3661 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     3721 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     3781 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     3841 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     3901 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3961 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4021 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4081 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4141 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     4201 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4261 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4321 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4381 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4441 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4501 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4561 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4621 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4681 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4741 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4801 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4861 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4921 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4981 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5041 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5101 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5161 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5221 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5281 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5341 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5401 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5461 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5521 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5581 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5641 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5701 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5761 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5821 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5881 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5941 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6001 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6061 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6121 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6181 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6241 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6301 tacggttcct ggccttttgc tggccttttg ctcacatgtc ctgcaggcag ctgcgcgctc
     6361 gctcgctcac tgaggccgcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc
     6421 gagcgagcgc gcagagaggg agtggccaac tccatcacta g
//
