LOCUS Exported 10565 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS TroponinT-GCaMP5-Zeo SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10565) AUTHORS Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, Christoforou N, Epstein JA, Gearhart JD TITLE Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. JOURNAL J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004. PUBMED 23591016 REFERENCE 2 (bases 1 to 10565) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10565 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" primer_bind 3220..3239 /label=T7 /note="T7 promoter, forward primer" promoter 3220..3238 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 3270..3294 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3304..3321 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 3325..3357 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 3361..3384 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" regulatory 3391..3400 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3412..3468 /codon_start=1 /product="calmodulin-binding peptide from avian smooth muscle myosin light chain kinase" /label=calmodulin-binding peptide /translation="RRKWNKTGHAVRAIGRLSS" primer_bind 3681..3702 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(3808..3829) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4069..4088) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" CDS 4204..4644 /codon_start=1 /product="calcium-binding protein" /label=calmodulin /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA ELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNGYISAAEL RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK" CDS 4654..4707 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" protein_bind complement(5089..5113) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5139..5180 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 5190..5207 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" misc_feature 5257..5845 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5310..5330) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5728..5739) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5848..5864) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5849..5865) /label=pBluescriptKS /note="For pBluescript vector" LTR 6370..6550 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6576..6593) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6582..6806 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6852..7280 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6939..6958) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7149..7170 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7289..7309) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7294..7623 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7474..7609 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7536..7555 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7671..7718 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7737..8111 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8241..8362 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8278..8297) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8332..8351 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8411..8427) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8411..8427) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8424..8446) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8435..8451 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8459..8489) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8504..8525 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8642..8659) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8813..9401) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8893..8912) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9572..10432) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10195..10214 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10433..10537) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta agtcatggag aagacccacc ttgcagatgt cctcactggg 2641 gctggcagag ccggcaacct gcccaaggct gctcagtcca ttaggagcca gtagcctgga 2701 agatgtcttt acccccagca tcagttcaag tggagcagca cataactctt gccctctgcc 2761 ttccaagatt ctggtgctga gacttatgga gtgtcttgga ggttgccttc tgccccccaa 2821 ccctgctccc agctggccct cccaggcctg ggttgctggc ctctgcttta tcaggattct 2881 caagagggac agctggttta tgttgcatga ctgttccctg catatctgct ctggttttaa 2941 atagcttatc tgagcagctg gaggaccaca tgggcttata tggcgtgggg tacatgttcc 3001 tgtagccttg tccctggcac ctgccaaaat agcagccaac accccccacc cccaccgcca 3061 tccccctgcc ccacccgtcc cctgtcgcac attcctccct ccgcagggct ggctcaccag 3121 gccccagccc acatgcctgc ttaaagccct ctccatcctc tgcctcaccc agtccccgct 3181 gagactgagc agacgcctcc aggatccgcc accctcgagt aatacgactc actataggga 3241 gacccaagct ggctaggtaa gcttgatcaa caagtttgta caaaaaagca ggctatgggt 3301 tctcatcatc atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg 3361 gatctgtacg acgatgacga taaggatctc gccaccatgg tcgactcgtc acgtcgtaag 3421 tggaataaga caggtcacgc agtcagagct ataggtcggc tgagctcact cgagaacgtc 3481 tatatcaagg ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac 3541 atcgaggacg gcggcgtgca gctcgcctac cactaccagc agaacacccc catcggcgac 3601 ggccccgtgc tgctgcccga caaccactac ctgagcgtgc agtccaaact ttcgaaagac 3661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 3721 ctcggcatgg acgagctgta caagggcggt accggaggga gcatggtgag caagggcgag 3781 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 3841 aagttcagcg tgtccggcga gggtgagggc gatgccacct acggcaagct gaccctgaag 3901 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc 3961 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag 4021 tccgccatgc ccgagggcta catccaggag cgcaccatct tcttcaagga cgacggcaac 4081 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgggctg 4141 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacctg 4201 ccggaccaac tgactgaaga gcagatcgca gaatttaaag aggctttctc cctatttgac 4261 aaggacgggg atgggacaat aacaaccaag gagctgggga cggtgatgcg gtctctgggg 4321 cagaacccca cagaagcaga gctgcaggac atgatcaatg aagtagatgc cgacggtgac 4381 ggcacaatcg acttccctga gttcctgaca atgatggcaa gaaaaatgaa atacacagac 4441 agtgaagaag aaattagaga agcgttccgt gtgtttgata aggatggcaa tggctacatc 4501 agtgcagcag agcttcgcca cgtgatgaca aaccttggag agaagttaac agatgaagag 4561 gttgatgaaa tgatcaggga agcagacatc gatggggacg gtcaggtaaa ctacgaagag 4621 tttgtacaaa tgatgacagc gaagggatcc ggagagggca gaggaagtct tctaacatgc 4681 ggtgacgtgg aggagaatcc cggcccttcc ggtatggcca aactcacttc tgcagtccca 4741 gtcctcacag caagggatgt tgcaggggct gtagagttct ggactgacag attaggattc 4801 tccagagact ttgttgaaga tgattttgct ggtgttgtca gagatgatgt caccctcttc 4861 atctcagcag ttcaggacca agttgtccct gacaacaccc ttgcttgggt ctgggtcaga 4921 ggcctagatg agctttatgc agaatggtca gaagtagtca gcacaaattt cagggatgcc 4981 tctggcccag ccatgacaga aattggtgaa caaccttggg gaagggaatt tgccctcaga 5041 gaccctgctg gaaattgtgt ccattttgta gctgaggaac aggactagac ccagctttct 5101 tgtacaaagt ggttgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc 5161 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc 5221 gctgatcagc ctcaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt 5281 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 5341 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 5401 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 5461 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 5521 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 5581 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 5641 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg 5701 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 5761 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 5821 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat 5881 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca 5941 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact 6001 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 6061 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac 6121 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga 6181 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag 6241 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta 6301 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg 6361 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 6421 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 6481 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 6541 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 6601 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 6661 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 6721 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 6781 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 6841 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 6901 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 6961 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 7021 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 7081 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 7141 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 7201 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 7261 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 7321 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 7381 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 7441 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 7501 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 7561 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 7621 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt 7681 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg 7741 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt 7801 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg 7861 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca 7921 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg 7981 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt 8041 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg 8101 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg 8161 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 8221 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 8281 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 8341 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 8401 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 8461 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 8521 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 8581 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 8641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 8701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 8761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 8821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 8881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 8941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 9001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 9061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 9121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 9181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 9241 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 9301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 9361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 9421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 9481 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 9541 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 9601 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 9661 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 9721 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 9781 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 9841 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 9901 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 9961 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 10021 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 10081 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 10141 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 10201 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 10261 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 10321 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 10381 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 10441 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 10501 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 10561 ctgac //