LOCUS Exported 5614 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSJS 1240 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5614) AUTHORS Rosalind Kim, Steve J. Sandler, Stanley Goldman, Hisao Yokota, Alvin J. Clark, Sung-Hou Kim TITLE Overexpression of archaeal proteins in Escherichia coli JOURNAL Biotechnology Letters. March 1998. 20(3): 207-210. REFERENCE 2 (bases 1 to 5614) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5614 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1..546 /direction=RIGHT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind 447..466 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 663..680 /label=L4440 /note="L4440 vector, forward primer" promoter 1072..1174 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 1175..1834 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1241..1260) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS complement(3673..4461) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK" tRNA complement(4981..5057) /gene="argU" /product="E. coli tRNA-Arg recognizing AGA and AGG" /label=argU tRNA 5304..5379 /gene="ileX" /product="E. coli tRNA-Ile recognizing AUA" /label=ileX ORIGIN 1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca 61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg 121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa 181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt 241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg 301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg 361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca 421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg 481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta 541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt 601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc 661 gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca 721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc 781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgct tttgccgtta 841 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga 901 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ccgacgcact 961 ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt 1021 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca 1081 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga 1141 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac 1201 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc 1261 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa 1321 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc 1381 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca 1441 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata 1501 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga 1561 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc 1621 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc 1681 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat 1741 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca 1801 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc 1861 ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc 1921 gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc 1981 tattgctggt ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat 2041 gcctgaggcc agtttgctca ggctctcccc gtggaggtaa taattgacga tatgatcatt 2101 tattctgcct cccagagcct gataaaaacg gttagcgctt cgttaataca gatgtaggtg 2161 ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg 2221 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgctgccg 2281 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 2341 tgccccgcgc ccaccggaag gagctaccgg cagcggtgcg gactgttgta actcagaata 2401 agaaatgagg ccgctcatgg cgttgactct cagtcatagt atcgtggtat caccggttgg 2461 ttccactctc tgttgcgggc aacttcagca gcacgtaggg gacttccgcg tttccagact 2521 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 2581 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 2641 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 2701 ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga 2761 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc 2821 aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg 2881 gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct 2941 acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc 3001 agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc 3061 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg 3121 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc 3181 aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa 3241 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc 3301 gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc 3361 cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg 3421 gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag 3481 ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg 3541 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcgagctcga cccgcatagt 3601 cttccccagc tctctaacgc ttgagttaag ccgcgccgcg aagcggcgtc ggcttgaacg 3661 aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtgaacaaa 3721 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tgtccaagat aagcctgcct 3781 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc 3841 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag 3901 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc 3961 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg 4021 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc 4081 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg 4141 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac 4201 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt 4261 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat 4321 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa 4381 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc 4441 ggcgatcacc gcttccctca tgatgtttaa ctcctgaatt aagccgcgcc gcgaagcggt 4501 gtcggcttga atgaattgtt aggcgtcatc ctgtgctccc gagaaccagt accagtacat 4561 cgctgtttcg ttcgagactt gaggtctagt tttatacgtg aacaggtcaa tgccgccgag 4621 agtaaagcca cattttgcgt acaaattgca ggcaggtaca ttgttcgttt gtgtctctaa 4681 tcgtatgcca aggagctgtc tgcttagtgc ccactttttc gcaaattcga tgagactgtg 4741 cgcgactcct ttgcctcggt gcgtgtgcga cacaacaatg tgttcgatag aggctagatc 4801 gttccatgtt gagttgagtt caatcttccc gacaagctct tggtcgatga atgcgccata 4861 gcaagcaggt cgagctcgca tgcgaggaaa atagacgaca tttttggtga taatgtccca 4921 aatatgtccc actctgaaat tatggaggat ataaagaagg cgtaactgat tgaattgtaa 4981 tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt tgctctatcc 5041 aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat tatacggtca 5101 acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga ccgcgtctga 5161 ttttctgatt tatttcgcta tagcggcaaa caaacgcaca ccgctgcgcg tctgaatcaa 5221 gaaaacccgt attttcatgt atcaaagtga cctgcagcca agcttggatt gcgacacgga 5281 gttactttat aatccaatcg attggcccct tagctcagtg gttagagcag gcgactcata 5341 atcgcttggt cgctggttca agtccagcag gggccaccag cggccgcaaa ggctgacgag 5401 aaatcgtcag cctttttaag ctttaagccg aattccagca cactggcggc cgttactagt 5461 ggatccgagc tcggtaccaa gcttatcgat gataagctgt caaacatgag aattacaact 5521 tatatcgtat ggggctgact tcaggtgcta catttgaaga gataaattgc actgaaatct 5581 agaaatattt tatctgatta ataagatgat cttc //