LOCUS Exported 5958 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses HsPDZ1b (Homo sapiens) folllwed by restriction sites. ACCESSION . VERSION . KEYWORDS pOVC1_X_HsPDZ1b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5958) AUTHORS Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H TITLE Engineering Strategy and Vector Library for the Rapid Generation of Modular Light-Controlled Protein-Protein Interactions. JOURNAL J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 10.1016/j.jmb.2019.05.033. PUBMED 31150735 REFERENCE 2 (bases 1 to 5958) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5958 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 129..508 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 509..712 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 663..683 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 757..776 /label=T7 /note="T7 promoter, forward primer" promoter 757..775 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1443..1460) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1449..1673 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1719..2147 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1806..1825) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2016..2037 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2156..2176) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2161..2490 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2341..2476 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2403..2422 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2557..3351 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2611..2630) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3221..3240 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 3525..3646 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3562..3581) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3616..3635 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3695..3711) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3695..3711) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3708..3730) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3719..3735 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3743..3773) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3788..3809 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3926..3943) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4097..4685) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4177..4196) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4856..5716) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5479..5498 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5717..5821) /gene="bla" /label=AmpR promoter primer_bind complement(5896..5915) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg 61 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata 121 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 181 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 241 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 301 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 361 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 421 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 481 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 541 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 601 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 661 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct 721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac 781 ccaagctgac catggccacc ggtgaattct caggctctgg atcccccggg ccagaacttg 841 gatttagcat atcaggtggt gtcgggggta gaggaaaccc attcagacct gatgatgatg 901 gtatatttgt aacaagggta caacctgaag gaccagcatc aaaattactg cagccaggtg 961 ataaaattat tcaggctaat ggctacagtt ttataaatat tgaacatgga caagcagtgt 1021 ccttgctaaa aactttccag aatacagttg aactcatcat tgtacgagaa gttggtaacg 1081 gtgcaaaaca agagattcga gtgagggttg aaaaagacgg tggttctgga ggcgtttctt 1141 ctgttccgac caatctggaa gttgttgctg cgaccccgac tagcctgctg atcagctggg 1201 atgcgtatag ggagcttccg gttagttatt accgtatcac gtacggtgaa acaggtggta 1261 actccccggt tcaggagttc actgtacctg gttccaagtc tactgctacc atcagcggcc 1321 tgaaaccggg tgtcgactat accatcactg tatacgcaca ttacaactac cattactact 1381 ctagcccaat ctcgattaac taccgtacct ctagacccgg gtaagtttaa accgctgatc 1441 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 1501 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 1561 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 1621 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 1681 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 2101 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 2161 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 2221 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 2281 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 2341 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 2401 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 2461 ggcttttttg gaggcctagg cttttgcaaa aagctctcgg gagcttgtat atccattttc 2521 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 2581 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 2641 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 2701 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 2761 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 2821 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 2881 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 2941 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 3001 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 3061 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 3121 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 3181 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 3241 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 3301 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 3361 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 3421 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 3481 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 3541 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 3601 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 3661 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 3721 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 3781 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 3841 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 3901 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3961 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4021 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4081 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4141 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4201 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4261 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4321 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4381 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 4441 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 4501 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 4561 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4621 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 4681 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 4741 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 4801 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 4861 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 4921 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 4981 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 5041 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 5101 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 5161 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 5221 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 5281 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 5341 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 5401 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 5461 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 5521 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 5581 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 5641 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 5701 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 5761 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 5821 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct 5881 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc 5941 tgcttgtgtg ttggaggt //