LOCUS       Exported                6101 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    17_pAAV-ProB1-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      545..554
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             551..1474
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1481..2197
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1526..1547)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1787..1806)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2134..2155
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2228..2816
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2281..2301)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2699..2710)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2848..3324
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3047..3066)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3364..3504
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3364..3493
                     /label=AAV2 ITR
     rep_origin      3579..4034
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3666..3685)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3876..3897
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4051..4070)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4170..4192
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4230..4248)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4316..4420
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4421..5281
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4639..4658)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5452..6040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5941..5960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttcacca agtaggagtc cttcagtaga tgaaaggggt
      181 attttaaacc gttgaagcta tcttggtggc aatctaggat gttgagacct cagagaaaat
      241 gtcgaaacct tggaaaattt attttgacga ttagaaattg tttaataata aaacaaaaga
      301 cttcctttct ccagccccac tttccctgtt tcttttgtca taggttgttg tgaactcgat
      361 ctgggaggga atcctggact cgcaacccga ctccgctcgg cgttgattgg ctcctgcctc
      421 aggaccccgc cggacccgcc ccccggccgt gggaatcgcc gcctagcagg cgggctgcgg
      481 ctgccactca gtcggagtgg cggaggccgt agccccgcct cctcccccct aattgatagg
      541 atccgccacc atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt
      601 aacgaaccca gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc
      661 gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg
      721 gcttgctgct ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc
      781 aacctgcggc tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga
      841 gttcttcttc gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca
      901 gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc atttgcattc acctgtcaaa
      961 cctgacgggc ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat
     1021 tggcacaatt gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt
     1081 cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat
     1141 cgagggttac cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg
     1201 gctcttcttc gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt
     1261 cggcgtcctg agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa
     1321 gaactgctgg ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat
     1381 ccacggcgac attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga
     1441 gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc atggtgagca agggcgagga
     1501 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1561 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1621 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1681 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1741 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1801 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1861 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1921 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1981 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2041 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2101 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2161 cgccgggatc actctcggca tggacgagct gtacaagtga taagaattcg atatcaagct
     2221 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2281 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2341 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2401 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2461 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2521 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2581 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2641 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2701 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2761 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2821 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     2881 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2941 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     3001 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     3061 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     3121 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     3181 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3241 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     3301 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     3361 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3421 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     3481 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     3541 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     3601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     3661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3721 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3781 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3841 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3901 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     3961 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4021 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4081 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4141 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4201 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4261 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4321 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4381 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4441 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4501 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4561 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4621 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4681 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4741 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4801 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4861 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4921 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4981 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5041 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5101 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5161 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5221 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5281 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5341 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5521 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5581 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5641 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5701 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5761 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5821 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5881 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5941 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6001 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6061 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
