LOCUS       Exported                6421 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    104_pAAV-ProC15-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        532..728
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      579..714
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     641..660
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      735..744
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             741..1664
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1671..2387
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1716..1737)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1977..1996)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2324..2345
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2418..3006
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2471..2491)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2889..2900)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3038..3514
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3237..3256)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3554..3694
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3554..3683
                     /label=AAV2 ITR
     rep_origin      3769..4224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3856..3875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4066..4087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4241..4260)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4360..4382
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4420..4438)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4506..4610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4611..5471
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4829..4848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5642..6230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6131..6150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6292..6421
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgtccatta tgtgagtaca ctcaagtgtc ccgaccctga ctttactcaa
       61 gtgtgttctc agatctccta ctcaagtgtg ttattttttg gaagactcaa gtgtgccata
      121 agttcactga ctcaagtgtc agatacccgt aagaactcaa gtgtaggact acaagaagca
      181 ctcaagtgta aaaattgtag ggaaactcaa gtgtctcgca attcactgta ctcaagtgta
      241 tcttctaaac atggactcaa gtgttctagc tattgtcaga ctcaagtgtt ttccggatcg
      301 ggacactcaa gtgtgttcat cgaaaagcca ctcaagtgta acaggaagca ctccactcaa
      361 gtgtcccgtc gagctagaga ctcaagtgtc attatcacga ggagactcaa gtgtggatgc
      421 aatcggggta ctcaagtgtg ttaggagact ctgaactcaa gtgtcgtgaa ctgtttacga
      481 ctcaagtgta ggtgcgataa gcccactcaa gtgtgctcga gatctgcgat ctgcatctca
      541 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
      601 gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg
      661 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
      721 tttgcaaagg atccgccacc atggattatg gaggcgccct gagtgccgtt gggcgcgagc
      781 tgctatttgt aacgaaccca gtagtcgtca atggctctgt acttgtgcct gaggaccagt
      841 gttactgcgc gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg
      901 tgctgcaatg gcttgctgct ggcttctcca tcctactgct tatgttttac gcctaccaaa
      961 catggaagtc aacctgcggc tgggaggaga tctatgtgtg cgctatcgag atggtcaagg
     1021 tgattctcga gttcttcttc gagtttaaga acccgtccat gctgtatcta gccacaggcc
     1081 accgcgtcca gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc atttgcattc
     1141 acctgtcaaa cctgacgggc ttgtccaacg actacagcag gcgcaccatg ggtctgcttg
     1201 tgtctgatat tggcacaatt gtgtggggcg ccacttccgc catggccacc ggatacgtca
     1261 aggtcatctt cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca
     1321 aggcctacat cgagggttac cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg
     1381 gcatggcttg gctcttcttc gtatcatggg gtatgttccc catcctgttc atcctcggcc
     1441 ccgagggctt cggcgtcctg agcgtgtacg gctccaccgt cggccacacc atcattgacc
     1501 tgatgtcgaa gaactgctgg ggtctgctcg gccactacct gcgcgtgctg atccacgagc
     1561 atatcctcat ccacggcgac attcgcaaga ccaccaaatt gaacattggt ggcactgaga
     1621 ttgaggtcga gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc atggtgagca
     1681 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1741 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1801 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1861 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1921 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1981 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2041 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2101 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2161 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2221 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2281 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2341 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga taagaattcg
     2401 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2461 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2521 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2581 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2641 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2701 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2761 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2821 cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct
     2881 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2941 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3001 ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc
     3061 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
     3121 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
     3181 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
     3241 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg
     3301 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac
     3361 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct
     3421 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt
     3481 acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg
     3541 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     3601 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     3661 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     3721 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     3781 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3841 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3901 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3961 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     4021 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4081 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     4141 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4201 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     4261 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4321 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4381 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     4441 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     4501 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4561 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4621 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4681 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4741 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4801 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4861 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4921 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4981 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5041 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5101 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5161 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5221 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5281 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5341 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5401 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5461 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5521 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5761 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tcctgcaggc
     6301 agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt tggtcgcccg
     6361 gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc
     6421 t
//
