LOCUS       Exported                6461 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    45_pAAV-ProC10-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        571..767
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      618..753
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     680..699
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      774..783
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             780..1703
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1710..2426
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1755..1776)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2016..2035)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2363..2384
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2457..3045
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2510..2530)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2928..2939)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3077..3553
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3276..3295)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3593..3733
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3593..3722
                     /label=AAV2 ITR
     rep_origin      3808..4263
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3895..3914)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4105..4126
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4280..4299)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4399..4421
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4459..4477)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4545..4649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4650..5510
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4868..4887)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5681..6269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6170..6189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6331..6460
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 cggccgcacg cgttgggtct gtcagaacat aaatcaataa tttgccgatt ttagtataaa
       61 tcaataacta ccattagagt ccataaatca ataaacacgt ctcattttga taaatcaata
      121 aataggagtc accgttataa atcaataata taggctcgga gatataaatc aataaggggg
      181 tggtgaaata ataaatcaat aaacaacggc ttacataata aatcaataat cgcgtcacgt
      241 cttgataaat caataacatg gtctgttctg tataaatcaa taaaaccctc taccaagaat
      301 aaatcaataa tggcccgatc tatatataaa tcaataaggg acacgggccc gaataaatca
      361 ataataaaat acgctaggaa taaatcaata aaccataata caatcaataa atcaataacc
      421 tcccctacac gacataaatc aataatgcca ctagggacct ataaatcaat aactagcaat
      481 gcttggcata aatcaataat ttctcacgaa gataataaat caataactga gttgcgatag
      541 cataaatcaa taagctcgag atctgcgatc tgcatctcaa ttagtcagca accatagtcc
      601 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      661 atcgctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      721 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagga tccgccacca
      781 tggattatgg aggcgccctg agtgccgttg ggcgcgagct gctatttgta acgaacccag
      841 tagtcgtcaa tggctctgta cttgtgcctg aggaccagtg ttactgcgcg ggctggattg
      901 agtcgcgtgg cacaaacggt gcccaaacgg cgtcgaacgt gctgcaatgg cttgctgctg
      961 gcttctccat cctactgctt atgttttacg cctaccaaac atggaagtca acctgcggct
     1021 gggaggagat ctatgtgtgc gctatcgaga tggtcaaggt gattctcgag ttcttcttcg
     1081 agtttaagaa cccgtccatg ctgtatctag ccacaggcca ccgcgtccag tggttgcgtt
     1141 acgccgagtg gcttctcacc tgcccggtca tttgcattca cctgtcaaac ctgacgggct
     1201 tgtccaacga ctacagcagg cgcaccatgg gtctgcttgt gtctgatatt ggcacaattg
     1261 tgtggggcgc cacttccgcc atggccaccg gatacgtcaa ggtcatcttc ttctgcctgg
     1321 gtctgtgtta tggtgctaac acgttctttc acgctgccaa ggcctacatc gagggttacc
     1381 acaccgtgcc gaagggccgg tgtcgccagg tggtgactgg catggcttgg ctcttcttcg
     1441 tatcatgggg tatgttcccc atcctgttca tcctcggccc cgagggcttc ggcgtcctga
     1501 gcgtgtacgg ctccaccgtc ggccacacca tcattgacct gatgtcgaag aactgctggg
     1561 gtctgctcgg ccactacctg cgcgtgctga tccacgagca tatcctcatc cacggcgaca
     1621 ttcgcaagac caccaaattg aacattggtg gcactgagat tgaggtcgag acgctggtgg
     1681 aggacgaggc cgaggctggc gcggtaccca tggtgagcaa gggcgaggag ctgttcaccg
     1741 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1801 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1861 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1921 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1981 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     2041 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     2101 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     2161 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     2221 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     2281 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     2341 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     2401 ctctcggcat ggacgagctg tacaagtgat aagaattcga tatcaagctt atcgataatc
     2461 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2521 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     2581 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2641 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     2701 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     2761 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2821 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     2881 atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     2941 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3001 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc
     3061 tgctcgagag atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct
     3121 ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt
     3181 gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg
     3241 gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc
     3301 agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc
     3361 tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt
     3421 tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca
     3481 ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc
     3541 cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc
     3601 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     3661 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     3721 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3781 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     3841 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
     3901 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3961 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4021 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4081 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
     4141 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
     4201 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     4261 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     4321 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4381 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4441 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4501 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4561 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4621 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4681 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4741 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4801 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4861 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4921 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4981 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5041 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5101 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5161 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5221 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5281 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5341 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5401 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5461 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5521 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5581 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5641 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5701 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5761 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5821 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5881 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5941 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6001 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6061 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6301 tggccttttg ctggcctttt gctcacatgt cctgcaggca gctgcgcgct cgctcgctca
     6361 ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg
     6421 cgcagagagg gagtggccaa ctccatcact aggggttcct g
//
