LOCUS Exported 7421 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 51_pAAV-ProC12-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7421) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 7421) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7421 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1532..1728 /label=SV40 promoter /note="SV40 early promoter" rep_origin 1579..1714 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1641..1660 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 1735..1744 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1741..2664 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 2671..3387 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2716..2737) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2977..2996) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3324..3345 /label=EGFP-C /note="EGFP, forward primer" misc_feature 3418..4006 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3471..3491) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3889..3900) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 4038..4514 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(4237..4256) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 4554..4694 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 4554..4683 /label=AAV2 ITR rep_origin 4769..5224 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4856..4875) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5066..5087 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5241..5260) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5360..5382 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5420..5438) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5506..5610 /gene="bla" /label=AmpR promoter CDS 5611..6471 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5829..5848) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6642..7230 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7131..7150 /label=pBR322ori-F /note="pBR322 origin, forward primer" repeat_region 7292..7421 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" ORIGIN 1 gcggccgcac gcgttcaagc ctcctaccgc ctttgttatg caaacatatc aaacgccctc 61 ctttgttatg caaaagggct ggaacggggc ctttgttatg caaatcgccc tccccgatcc 121 ctttgttatg caaatttgac gaattcccac ctttgttatg caaacaaatc tcctaccctc 181 ctttgttatg caaagtgaga ggggctgcac ctttgttatg caaaatgcgg cccctgagac 241 ctttgttatg caaaaaccat gtacgcttgc ctttgttatg caaaccgcct gttgcttggc 301 ctttgttatg caaagccacg cgattggcgc ctttgttatg caaagctcgg ttatgtacac 361 ctttgttatg caaagctact ttaaacttgc ctttgttatg caaatcacga cctgaccgtc 421 ctttgttatg caaaaacggt tgaaatagtc ctttgttatg caaaatgata ttgaatagtc 481 ctttgttatg caaaaattta gatgccgacc ctttgttatg caaaggaatg ggcgtgctgc 541 ctttgttatg caaattttcg ctgcgacagc ctttgttatg caaacatgct cgccactcac 601 ctttgttatg caaaggtcta acaatgaccc ctttgttatg caaactacgt ggaatagatc 661 ctttgttatg caaaccccga gtttttgaac ctttgttatg caaaatcagt aacttcattc 721 ctttgttatg caaaatgtga cttaacctcc ctttgttatg caaatacacg ctagtcgcat 781 ggacgtggat gctagctaaa acccagcagg ctcggttgtt gtagttctta cataatgact 841 atcacctcgg caagggagtt ttaagcggca gcgggagaat tgccgcatga acacaaactt 901 tctctgtcga tggttcgcgg aactcttcgg cgattgtccg cccctttcaa ccacctaaat 961 agatgtgctt tttgggttga ggtgctgttc caaagaactg gcacgcatcg aagcacgtca 1021 aaatcggctc gtagaaattc gcacgtattc cccgtgcctg gtgggtcgtc gagattctgt 1081 gaaggagcac tgggaacgaa tgcgtgggtc accatacccg tagaatgctt gtaacgccac 1141 tagcggcttg gtcatctttc cctaaaggct gcgtggtccc gaatgacgta acgtcgatgg 1201 ccggttagcc caaaaatatg cccgtattta ctaaacctgt cgtgtctctc ttcctgttgt 1261 ctctcgctag gtccatccct aagtcttatc gaaggtgcta ttttactctg ctcttggaac 1321 acacccatct ccacgcacgt gataggtaca gcaagcgtta caggccatcg tttcgtacga 1381 attttcgaaa agtaatcctc agaaatcgaa tattaccttt agttggaacg cgcttccaaa 1441 gcgccgggcc ctgaagtgct atgggatggg cttctttcgc gtgtcctttt ggccgatgtc 1501 tgactgacag attagctcga gatctgcgat ctgcatctca attagtcagc aaccatagtc 1561 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 1621 catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 1681 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg atccgccacc 1741 atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca 1801 gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc gggctggatt 1861 gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct 1921 ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc aacctgcggc 1981 tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga gttcttcttc 2041 gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca gtggttgcgt 2101 tacgccgagt ggcttctcac ctgcccggtc atttgcattc acctgtcaaa cctgacgggc 2161 ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt 2221 gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt cttctgcctg 2281 ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat cgagggttac 2341 cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc 2401 gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg 2461 agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa gaactgctgg 2521 ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat ccacggcgac 2581 attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg 2641 gaggacgagg ccgaggctgg cgcggtaccc atggtgagca agggcgagga gctgttcacc 2701 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 2761 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 2821 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 2881 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 2941 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 3001 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 3061 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 3121 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 3181 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 3241 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 3301 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 3361 actctcggca tggacgagct gtacaagtga taagaattcg atatcaagct tatcgataat 3421 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 3481 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 3541 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 3601 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 3661 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 3721 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 3781 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc 3841 tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 3901 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 3961 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg 4021 ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc 4081 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt 4141 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg 4201 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg 4261 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc 4321 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt 4381 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc 4441 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc 4501 ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc 4561 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg 4621 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg 4681 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc 4741 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg 4801 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct 4861 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 4921 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 4981 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 5041 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 5101 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta 5161 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca 5221 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga 5281 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 5341 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 5401 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata 5461 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 5521 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 5581 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 5641 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 5701 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 5761 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 5821 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 5881 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 5941 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 6001 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 6061 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 6121 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 6181 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 6241 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 6301 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 6361 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 6421 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 6481 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 6541 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 6601 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 6661 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 6721 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 6781 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 6841 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 6901 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 6961 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 7021 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 7081 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 7141 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 7201 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 7261 ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc 7321 actgaggccg cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc 7381 gcgcagagag ggagtggcca actccatcac taggggttcc t //