LOCUS Exported 6546 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 11_pAAV-ProC6-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6546) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6546) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6546 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 787..983 /label=SV40 promoter /note="SV40 early promoter" rep_origin 834..969 /label=SV40 ori /note="SV40 origin of replication" primer_bind 896..915 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 990..999 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 996..1919 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1926..2642 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1971..1992) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2232..2251) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2579..2600 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2673..3261 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2726..2746) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3144..3155) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3293..3769 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3492..3511) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3809..3949 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3809..3938 /label=AAV2 ITR rep_origin 4024..4479 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4111..4130) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4321..4342 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4496..4515) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4615..4637 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4675..4693) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4761..4865 /gene="bla" /label=AmpR promoter CDS 4866..5726 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5084..5103) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5897..6485 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6386..6405 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtacgtgt tacgtgacgc gcacgtggca ctcagtaggc 181 ggcacgcacg tggcgtgatg cacccgtcac gcacgtggct gatagatcat ggcccgcacg 241 tggcgtatct gcaaccaacc gcacgtggcc cgaaaaatcg gcggcgcacg tggcctttac 301 ggctttagtc gcacgtggcg ccatcaaggt agtccgcacg tggctcgttt gtcgatatgc 361 gcacgtggct tgcattcgac ccgtcgcacg tggccgctga aaggtttagc gcacgtggcc 421 cctacgtcct acaccgcacg tggcgggtgg caaccacccc gcacgtggcg ccctgactct 481 tgaacgcacg tggccaaaga tgttccgctc gcacgtggcc aagggcctgg ggtgcgcacg 541 tggccgtatg gcgtcggacc gcacgtggca gagaaccttg ttgacgcacg tggccccata 601 ggtctcaggc gcacgtggcg ctgtgtaaat tagacgcacg tggctaaata tcggtcttgc 661 gcacgtggcg atttgggaat agtccgcacg tggcttaacc tcagaatgtc gcacgtggct 721 actggtcgac ggctcgcacg tggcgcatta cgttaccagc gcacgtggcg ctcgagatct 781 gcgatctgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 841 ccctaactcc gcccagttcc gcccattctc cgccccatcg ctgactaatt ttttttattt 901 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 961 ttggaggcct aggcttttgc aaaggatccg ccaccatgga ttatggaggc gccctgagtg 1021 ccgttgggcg cgagctgcta tttgtaacga acccagtagt cgtcaatggc tctgtacttg 1081 tgcctgagga ccagtgttac tgcgcgggct ggattgagtc gcgtggcaca aacggtgccc 1141 aaacggcgtc gaacgtgctg caatggcttg ctgctggctt ctccatccta ctgcttatgt 1201 tttacgccta ccaaacatgg aagtcaacct gcggctggga ggagatctat gtgtgcgcta 1261 tcgagatggt caaggtgatt ctcgagttct tcttcgagtt taagaacccg tccatgctgt 1321 atctagccac aggccaccgc gtccagtggt tgcgttacgc cgagtggctt ctcacctgcc 1381 cggtcatttg cattcacctg tcaaacctga cgggcttgtc caacgactac agcaggcgca 1441 ccatgggtct gcttgtgtct gatattggca caattgtgtg gggcgccact tccgccatgg 1501 ccaccggata cgtcaaggtc atcttcttct gcctgggtct gtgttatggt gctaacacgt 1561 tctttcacgc tgccaaggcc tacatcgagg gttaccacac cgtgccgaag ggccggtgtc 1621 gccaggtggt gactggcatg gcttggctct tcttcgtatc atggggtatg ttccccatcc 1681 tgttcatcct cggccccgag ggcttcggcg tcctgagcgt gtacggctcc accgtcggcc 1741 acaccatcat tgacctgatg tcgaagaact gctggggtct gctcggccac tacctgcgcg 1801 tgctgatcca cgagcatatc ctcatccacg gcgacattcg caagaccacc aaattgaaca 1861 ttggtggcac tgagattgag gtcgagacgc tggtggagga cgaggccgag gctggcgcgg 1921 tacccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 1981 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 2041 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 2101 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca 2161 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 2221 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 2281 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 2341 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 2401 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 2461 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 2521 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 2581 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 2641 agtgataaga attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg 2701 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 2761 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 2821 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 2881 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 2941 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 3001 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 3061 cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg 3121 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 3181 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 3241 ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca 3301 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac 3361 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa 3421 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag 3481 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc 3541 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt 3601 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac 3661 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc 3721 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta 3781 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc 3841 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc 3901 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg 3961 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat 4021 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 4081 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg 4141 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 4201 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg 4261 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 4321 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt 4381 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat 4441 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa 4501 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 4561 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 4621 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 4681 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 4741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 4801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 4861 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 4921 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 4981 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 5041 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 5101 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 5161 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 5221 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 5281 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 5341 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 5401 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 5461 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 5521 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 5581 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 5641 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 5701 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 5761 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 5821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 5881 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 5941 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 6001 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 6061 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 6121 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 6181 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 6241 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 6301 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 6361 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 6421 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 6481 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 6541 acatgt //