LOCUS       Exported                6401 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    124_pAAV-ProC3-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        512..708
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      559..694
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     621..640
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      715..724
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             721..1644
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1651..2367
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1696..1717)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1957..1976)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2304..2325
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2398..2986
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2451..2471)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2869..2880)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3018..3494
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3217..3236)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3534..3674
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3534..3663
                     /label=AAV2 ITR
     rep_origin      3749..4204
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3836..3855)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4046..4067
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4221..4240)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4340..4362
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4400..4418)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4486..4590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4591..5451
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4809..4828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5622..6210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6111..6130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6272..6401
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgtaaagct ggaactgggg ccggaaacag ctgagggtgc ccagccggaa
       61 actcgaaatc aacgtaggcc ggaaactatt cgatgaattc cgccggaaac taggtccgga
      121 ggactgccgg aaacacctgt aatcaagccg ccggaaacct gttgtggccg tatgccggaa
      181 acgtcttaat tggacgtgcc ggaaactctt ttaatgagtt cgccggaaac cagaccagcc
      241 gagctgccgg aaaccggtta tatagaacgg ccggaaacgg tccacaggaa aaagccggaa
      301 acacccaaac ggttagcgcc ggaaacgact ggggaggacg tgccggaaac gtactatctg
      361 aagatgccgg aaacacttga aagctccaag ccggaaacgg gcccgtgcgg atagccggaa
      421 actgacggta cacggccgcc ggaaacacta cttgtatggt agccggaaac ttgggcgtgg
      481 ctggggccgg aaacgctcga gatctgcgat ctgcatctca attagtcagc aaccatagtc
      541 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
      601 catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
      661 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg atccgccacc
      721 atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca
      781 gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc gggctggatt
      841 gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct
      901 ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc aacctgcggc
      961 tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga gttcttcttc
     1021 gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca gtggttgcgt
     1081 tacgccgagt ggcttctcac ctgcccggtc atttgcattc acctgtcaaa cctgacgggc
     1141 ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt
     1201 gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt cttctgcctg
     1261 ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat cgagggttac
     1321 cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc
     1381 gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg
     1441 agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa gaactgctgg
     1501 ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat ccacggcgac
     1561 attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg
     1621 gaggacgagg ccgaggctgg cgcggtaccc atggtgagca agggcgagga gctgttcacc
     1681 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1741 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1801 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1861 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1921 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1981 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2041 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2101 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2161 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2221 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2281 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2341 actctcggca tggacgagct gtacaagtga taagaattcg atatcaagct tatcgataat
     2401 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2461 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2521 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2581 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2641 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2701 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2761 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     2821 tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2881 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     2941 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg
     3001 ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     3061 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     3121 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     3181 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     3241 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     3301 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     3361 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     3421 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     3481 ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc
     3541 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     3601 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     3661 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3721 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     3781 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     3841 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3901 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3961 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4021 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4081 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     4141 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     4201 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     4261 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4321 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4381 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4441 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4501 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4561 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4621 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4681 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4741 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4801 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4861 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4921 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4981 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5041 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5101 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5161 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5221 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5281 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5341 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5401 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5461 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5521 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5581 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5641 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5701 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5761 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5821 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5881 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5941 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6001 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6061 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6121 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6181 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6241 ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc
     6301 actgaggccg cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
     6361 gcgcagagag ggagtggcca actccatcac taggggttcc t
//
