LOCUS       Exported                8255 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Constitutive expression of c-myc-FPR1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pBGSA c-myc-FPR1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Gripentrog JM, Kantele KP, Jesaitis AJ, Miettinen HM
  TITLE     Experimental evidence for lack of homodimerization of the G 
            protein-coupled human N-formyl peptide receptor.
  JOURNAL   J Immunol. 2003 Sep 15;171(6):3187-93.
  PUBMED    12960347
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      159..294
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             324..592
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          632..728
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             786..815
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1890..1906)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1890..1906)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2071..2090)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     promoter        complement(2419..2437)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2458..2474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2458..2474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2471..2493)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2482..2498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2506..2536)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2551..2572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2689..2706)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2860..3448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2940..2959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3619..4479)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4242..4261
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4480..4584)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4610..5065)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4747..4768)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4959..4978
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5192..5214
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5206..5223
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5207..5223
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5230..5249
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5230..5248
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5285..5419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5309..5328)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5363..5382
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5844..5864)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5994..6059
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(6479..7273)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6590..6609)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7200..7219
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7630..7959)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7644..7779
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8002..8024)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8165..8184
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8234..592
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
ORIGIN
        1 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
       61 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
      121 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
      181 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
      241 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
      301 tttgcaaaaa gctcctcgat cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
      361 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
      421 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
      481 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
      541 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctcc
      601 tcgaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt cttttatttc
      661 aggtcccgga tccggtggtg gtgcaaatca aagaactgct cctcagtgga tgttgccttt
      721 acttctaggc ctgtacggaa gtgttacttc tgctctaaaa gctgcggaat tcactagtga
      781 ttatggagca gaagctgatc tccgaggaag acctgatgga gacaaattcc tctctcccca
      841 cgaacatctc tggagggaca cctgctgtat ctgctggcta tctcttcctg gatatcatca
      901 cttatctggt atttgcagtc acctttgtcc tcggggtcct gggcaacggg cttgtgatct
      961 gggtggctgg attccggatg acacacacag tcaccaccat cagttacctg aacctggccg
     1021 tggctgactt ctgtttcacc tccactttgc cattcttcat ggtcaggaag gccatgggag
     1081 gacattggcc tttcggctgg ttcctgtgca aattcgtctt taccatagtg gacatcaact
     1141 tgttcggaag tgtcttcctg atcgccctca ttgctctgga ccgctgtgtt tgcgtcctgc
     1201 atccagtctg gacccagaac caccgcaccg tgagcctggc caagaaggtg atcattgggc
     1261 cctgggtgat ggctctgctc ctcacattgc cagttatcat tcgtgtgact acagtacctg
     1321 gtaaaacggg gacagtagcc tgcactttta acttttcgcc ctggaccaac gaccctaaag
     1381 agaggataaa tgtggccgtt gccatgttga cggtgagagg catcatccgg ttcatcattg
     1441 gcttcagcgc acccatgtcc atcgttgctg tcagttatgg gcttattgcc accaagatcc
     1501 acaagcaagg cttgattaag tccagtcgtc ccttacgggt cctctccttt gtcgcagcag
     1561 ccttttttct ctgctggtcc ccatatcagg tggtggccct tatagccaca gtcagaatcc
     1621 gtgagttatt gcaaggcatg tacaaagaaa ttggtattgc agtggatgtg acaagtgccc
     1681 tggccttctt caacagctgc ctcaacccca tgctctatgt cttcatgggc caggacttcc
     1741 gggagaggct gatccacgcc cttcccgcca gtctggagag ggccctgacc gaggactcaa
     1801 cccaaaccag tgacacagct accaattcta ctttaccttc tgcagaggtg gagttacagg
     1861 caaagtaata gaattcctgc agcccggggg atccactagt tctagacctg gccttggaag
     1921 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     1981 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     2041 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     2101 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattccc ctgcctcagc
     2161 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     2221 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     2281 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     2341 ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc
     2401 gagctccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc
     2461 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2521 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2581 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2641 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2701 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2761 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2821 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2881 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2941 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3001 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3061 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3121 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3181 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3241 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3301 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3361 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3421 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3481 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3541 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3601 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3661 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3721 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3781 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3841 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3901 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3961 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4021 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4081 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4141 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4201 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4261 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4321 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4381 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4441 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4501 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4561 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     4621 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     4681 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     4741 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4801 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4861 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4921 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4981 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     5041 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     5101 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     5161 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     5221 agtgaattgt aatacgactc actatagggc gaattgggta ccgggccccc cctcgaggtc
     5281 gaccgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     5341 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     5401 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     5461 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     5521 tgatctctag tcaaggcact atacatcaaa tattccttat taaccccttt acaaattaaa
     5581 aagctaaagg tacacaattt ttgagcatag ttattaatag cagacactct atgcctgtgt
     5641 ggagtaagaa aaaacagtat gttatgatta taactgttat gcctacttat aaaggttaca
     5701 gaatattttt ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata
     5761 gcaaagcaag caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct
     5821 gaaggaaagt ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga
     5881 gagtcagcag tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc
     5941 tcattaaagg cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat
     6001 acacaaacaa ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc
     6061 ttagagcttt aaatctctgt aggtagtttg tccaattatg tcacaccaca gaagtaaggt
     6121 tccttcacaa agatccggac caaagcggcc atcgtgcctc cccactcctg cagttcgggg
     6181 gcatggatgc gcggatagcc gctgctggtt tcctggatgc cgacggattt gcactgccgg
     6241 tagaactccg cgaggtcgtc cagcctcagg cagcagctga accaactcgc gaggggatcg
     6301 agcccggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg
     6361 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa
     6421 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc
     6481 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     6541 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     6601 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     6661 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     6721 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
     6781 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     6841 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     6901 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     6961 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     7021 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     7081 ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     7141 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     7201 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     7261 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc
     7321 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     7381 taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt
     7441 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt
     7501 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     7561 tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc
     7621 gtgaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     7681 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     7741 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     7801 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     7861 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     7921 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct gcctcgcgcg
     7981 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     8041 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     8101 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     8161 tatgcggcat cagagcagat tgtactgaga gtgcaccata ggtcgacggt atcgataagc
     8221 ttggctgtgg aatgtgtgtc agttagggtg tggaa
//
