LOCUS Exported 4689 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: C1 Cloning Vector, Excitation: 558, Emission: 583. ACCESSION . VERSION . KEYWORDS DsRed2-C1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4689) TITLE Michael Davidson Empty Backbones REFERENCE 2 (bases 1 to 4689) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4689 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 106..409 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 410..613 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 564..584 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 652..661 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 658..1332 /codon_start=1 /product="improved tetrameric variant of DsRed fluorescent protein" /label=DsRed2 /note="mammalian codon-optimized" /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH LFL" primer_bind complement(838..858) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 1250..1273 /label=DsRed1-C /note="DsRed1, forward primer" misc_feature 1333..1398 /label=MCS /note="multiple cloning site" polyA_signal 1522..1643 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1559..1578) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1613..1632 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1650..2105) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1787..1808) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1999..2018 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2132..2236 /gene="bla" /label=AmpR promoter promoter 2238..2595 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2261..2281) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2446..2581 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2508..2527 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2630..3424 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2684..2703) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3294..3313 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3612..3631) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3656..3703 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4032..4620 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4521..4540 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta 61 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac 121 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac 181 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt 241 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat 301 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga 361 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt 421 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca 481 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg 541 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta 601 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg 661 gcctcctccg agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc 721 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac 781 aacaccgtga agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg 841 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac 901 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 961 ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag 1021 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg 1081 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc 1141 cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac 1201 atggccaaga agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc 1261 acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac 1321 cacctgttcc tgtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt 1381 accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca taccacattt 1441 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 1501 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 1561 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 1621 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt 1681 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 1741 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 1801 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 1861 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 1921 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 1981 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 2041 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 2101 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 2161 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 2221 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg 2281 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 2341 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 2401 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 2461 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 2521 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 2581 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca 2641 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 2701 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 2761 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 2821 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 2881 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 2941 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 3001 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 3061 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 3121 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 3181 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 3241 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 3301 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 3361 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 3421 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 3481 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 3541 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg 3601 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 3661 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg 3721 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 3781 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 3841 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat 3901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 3961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 4021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 4081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 4141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 4201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 4261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 4321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 4381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 4441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 4501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 4561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 4621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 4681 ttctttcct //