LOCUS Exported 9856 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian Target-ACE expression vector. ACCESSION . VERSION . KEYWORDS pRS0045 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9856) AUTHORS Sakata RC, Ishiguro S, Mori H, Tanaka M, Tatsuno K, Ueda H, Yamamoto S, Seki M, Masuyama N, Nishida K, Nishimasu H, Arakawa K, Kondo A, Nureki O, Tomita M, Aburatani H, Yachie N TITLE Base editors for simultaneous introduction of C-to-T and A-to-G mutations. JOURNAL Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. PUBMED 32483365 REFERENCE 2 (bases 1 to 9856) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9856 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 482..4582 /codon_start=1 /product="nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9(D10A) /note="generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 4790..4855 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 5489..5509 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5516..5770 /codon_start=1 /product="uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995)" /label=UGI /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" primer_bind complement(5793..5810) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5799..6023 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(6094..6110) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6094..6110) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6107..6129) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6118..6134 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6142..6172) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6187..6208 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6325..6342) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6496..7084) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6576..6595) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7255..8115) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7878..7897 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8116..8220) /gene="bla" /label=AmpR promoter primer_bind complement(8299..8318) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 8490..8869 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 8870..9073 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 9024..9044 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 9115..9134 /label=T7 /note="T7 promoter, forward primer" promoter 9115..9133 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 9147..481 /codon_start=1 /product="adenine base editor comprising wild-type E. coli TadA fused to the evolved TadA*(7.10) mutant (Gaudelli et al., 2017)" /label=ABE(7.10) /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGW NRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVF GARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQ SSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGSSEVEFSHEYWMRHALTLAKRARD EREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVT FEPCVMCAGAMIHSRIGRVVFGVRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECA ALLCYFFRMPRQVFNAQKKAQSSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGS" ORIGIN 1 cgtaatcggc gaaggttgga atagggcaat cggactccac gaccccactg cacatgcgga 61 aatcatggcc cttcgacagg gagggcttgt gatgcagaat tatcgactta tcgatgcgac 121 gctgtacgtc acgtttgaac cttgcgtaat gtgcgcggga gctatgattc actcccgcat 181 tggacgagtt gtattcggtg ttcgcaacgc caagacgggt gccgcaggtt cactgatgga 241 cgtgctgcat tacccaggca tgaaccaccg ggtagaaatc acagaaggca tattggcgga 301 cgaatgtgcg gcgctgttgt gttacttttt tcgcatgccc aggcaggtct ttaacgccca 361 gaaaaaagca caatcctcta ctgactctgg tggttcttct ggtggttcta gcggcagcga 421 gactcccggg acctcagagt ccgccacacc cgaaagttct ggtggttctt ctggtggttc 481 tgataaaaag tattctattg gtttagccat cggcactaat tccgttggat gggctgtcat 541 aaccgatgaa tacaaagtac cttcaaagaa atttaaggtg ttggggaaca cagaccgtca 601 ttcgattaaa aagaatctta tcggtgccct cctattcgat agtggcgaaa cggcagaggc 661 gactcgcctg aaacgaaccg ctcggagaag gtatacacgt cgcaagaacc gaatatgtta 721 cttacaagaa atttttagca atgagatggc caaagttgac gattctttct ttcaccgttt 781 ggaagagtcc ttccttgtcg aagaggacaa gaaacatgaa cggcacccca tctttggaaa 841 catagtagat gaggtggcat atcatgaaaa gtacccaacg atttatcacc tcagaaaaaa 901 gctagttgac tcaactgata aagcggacct gaggttaatc tacttggctc ttgcccatat 961 gataaagttc cgtgggcact ttctcattga gggtgatcta aatccggaca actcggatgt 1021 cgacaaactg ttcatccagt tagtacaaac ctataatcag ttgtttgaag agaaccctat 1081 aaatgcaagt ggcgtggatg cgaaggctat tcttagcgcc cgcctctcta aatcccgacg 1141 gctagaaaac ctgatcgcac aattacccgg agagaagaaa aatgggttgt tcggtaacct 1201 tatagcgctc tcactaggcc tgacaccaaa ttttaagtcg aacttcgact tagctgaaga 1261 tgccaaattg cagcttagta aggacacgta cgatgacgat ctcgacaatc tactggcaca 1321 aattggagat cagtatgcgg acttattttt ggctgccaaa aaccttagcg atgcaatcct 1381 cctatctgac atactgagag ttaatactga gattaccaag gcgccgttat ccgcttcaat 1441 gatcaaaagg tacgatgaac atcaccaaga cttgacactt ctcaaggccc tagtccgtca 1501 gcaactgcct gagaaatata aggaaatatt ctttgatcag tcgaaaaacg ggtacgcagg 1561 ttatattgac ggcggagcga gtcaagagga attctacaag tttatcaaac ccatattaga 1621 gaagatggat gggacggaag agttgcttgt aaaactcaat cgcgaagatc tactgcgaaa 1681 gcagcggact ttcgacaacg gtagcattcc acatcaaatc cacttaggcg aattgcatgc 1741 tatacttaga aggcaggagg atttttatcc gttcctcaaa gacaatcgtg aaaagattga 1801 gaaaatccta acctttcgca taccttacta tgtgggaccc ctggcccgag ggaactctcg 1861 gttcgcatgg atgacaagaa agtccgaaga aacgattact ccatggaatt ttgaggaagt 1921 tgtcgataaa ggtgcgtcag ctcaatcgtt catcgagagg atgaccaact ttgacaagaa 1981 tttaccgaac gaaaaagtat tgcctaagca cagtttactt tacgagtatt tcacagtgta 2041 caatgaactc acgaaagtta agtatgtcac tgagggcatg cgtaaacccg cctttctaag 2101 cggagaacag aagaaagcaa tagtagatct gttattcaag accaaccgca aagtgacagt 2161 taagcaattg aaagaggact actttaagaa aattgaatgc ttcgattctg tcgagatctc 2221 cggggtagaa gatcgattta atgcgtcact tggtacgtat catgacctcc taaagataat 2281 taaagataag gacttcctgg ataacgaaga gaatgaagat atcttagaag atatagtgtt 2341 gactcttacc ctctttgaag atcgggaaat gattgaggaa agactaaaaa catacgctca 2401 cctgttcgac gataaggtta tgaaacagtt aaagaggcgt cgctatacgg gctggggacg 2461 attgtcgcgg aaacttatca acgggataag agacaagcaa agtggtaaaa ctattctcga 2521 ttttctaaag agcgacggct tcgccaatag gaactttatg cagctgatcc atgatgactc 2581 tttaaccttc aaagaggata tacaaaaggc acaggtttcc ggacaagggg actcattgca 2641 cgaacatatt gcgaatcttg ctggttcgcc agccatcaaa aagggcatac tccagacagt 2701 caaagtagtg gatgagctag ttaaggtcat gggacgtcac aaaccggaaa acattgtaat 2761 cgagatggca cgcgaaaatc aaacgactca gaaggggcaa aaaaacagtc gagagcggat 2821 gaagagaata gaagagggta ttaaagaact gggcagccag atcttaaagg agcatcctgt 2881 ggaaaatacc caattgcaga acgagaaact ttacctctat tacctacaaa atggaaggga 2941 catgtatgtt gatcaggaac tggacataaa ccgtttatct gattacgacg tcgatcacat 3001 tgtaccccaa tcctttttga aggacgattc aatcgacaat aaagtgctta cacgctcgga 3061 taagaaccga gggaaaagtg acaatgttcc aagcgaggaa gtcgtaaaga aaatgaagaa 3121 ctattggcgg cagctcctaa atgcgaaact gataacgcaa agaaagttcg ataacttaac 3181 taaagctgag aggggtggct tgtctgaact tgacaaggcc ggatttatta aacgtcagct 3241 cgtggaaacc cgccaaatca caaagcatgt tgcacagata ctagattccc gaatgaatac 3301 gaaatacgac gagaacgata agctgattcg ggaagtcaaa gtaatcactt taaagtcaaa 3361 attggtgtcg gacttcagaa aggattttca attctataaa gttagggaga taaataacta 3421 ccaccatgcg cacgacgctt atcttaatgc cgtcgtaggg accgcactca ttaagaaata 3481 cccgaagcta gaaagtgagt ttgtgtatgg tgattacaaa gtttatgacg tccgtaagat 3541 gatcgcgaaa agcgaacagg agataggcaa ggctacagcc aaatacttct tttattctaa 3601 cattatgaat ttctttaaga cggaaatcac tctggcaaac ggagagatac gcaaacgacc 3661 tttaattgaa accaatgggg agacaggtga aatcgtatgg gataagggcc gggacttcgc 3721 gacggtgaga aaagttttgt ccatgcccca agtcaacata gtaaagaaaa ctgaggtgca 3781 gaccggaggg ttttcaaagg aatcgattct tccaaaaagg aatagtgata agctcatcgc 3841 tcgtaaaaag gactgggacc cgaaaaagta cggtggcttc gatagcccta cagttgccta 3901 ttctgtccta gtagtggcaa aagttgagaa gggaaaatcc aagaaactga agtcagtcaa 3961 agaattattg gggataacga ttatggagcg ctcgtctttt gaaaagaacc ccatcgactt 4021 ccttgaggcg aaaggttaca aggaagtaaa aaaggatctc ataattaaac taccaaagta 4081 tagtctgttt gagttagaaa atggccgaaa acggatgttg gctagcgccg gagagcttca 4141 aaaggggaac gaactcgcac taccgtctaa atacgtgaat ttcctgtatt tagcgtccca 4201 ttacgagaag ttgaaaggtt cacctgaaga taacgaacag aagcaacttt ttgttgagca 4261 gcacaaacat tatctcgacg aaatcataga gcaaatttcg gaattcagta agagagtcat 4321 cctagctgat gccaatctgg acaaagtatt aagcgcatac aacaagcaca gggataaacc 4381 catacgtgag caggcggaaa atattatcca tttgtttact cttaccaacc tcggcgctcc 4441 agccgcattc aagtattttg acacaacgat agatcgcaaa cgatacactt ctaccaagga 4501 ggtgctagac gcgacactga ttcaccaatc catcacggga ttatatgaaa ctcggataga 4561 tttgtcacag cttgggggtg acggtggagg aggtaccggc ggtggaggct cagcagaata 4621 cgtacgagct ctgtttgact tcaatgggaa tgacgaggag gatctcccct ttaagaaggg 4681 cgatattctc cgcatcagag ataagcccga agaacaatgg tggaatgccg aggatagcga 4741 agggaaaagg ggcatgattc tggtgccata tgtggagaaa tattccggtg actacaaaga 4801 ccatgatggg gattacaaag accacgacat cgactacaaa gacgacgacg ataaatcagg 4861 gatgacagac gccgagtacg tgcgcattca tgagaaactg gatatttaca ccttcaagaa 4921 gcagttcttc aacaacaaga aatctgtgtc acaccgctgc tacgtgctgt ttgagttgaa 4981 gcgaaggggc gaaagaaggg cttgcttttg gggctatgcc gtcaacaagc cccaaagtgg 5041 caccgagaga ggaatacacg ctgagatatt cagtatccga aaggtggaag agtatcttcg 5101 ggataatcct gggcagttta cgatcaactg gtattccagc tggagtcctt gcgctgattg 5161 tgccgagaaa attctggaat ggtataatca ggaacttcgg ggaaacgggc acacattgaa 5221 aatctgggcc tgcaagctgt actacgagaa gaatgcccgg aaccagatag gactctggaa 5281 tctgagggac aatggtgtag gcctgaacgt gatggtttcc gagcactatc agtgttgtcg 5341 gaagattttc atccaaagct ctcataacca gctcaatgaa aaccgctggt tggagaaaac 5401 actgaaacgt gcggagaagt ggagatccga gctgagcatc atgatccagg tcaagattct 5461 gcataccact aagtctccag ccgttggtcc caagaagaaa agaaaagtcg gtaccatgac 5521 caacctttcc gacatcatag agaaggaaac aggcaaacag ttggtcatcc aagagtcgat 5581 actcatgctt cctgaagaag ttgaggaggt cattgggaat aagccggaaa gtgacattct 5641 cgtacacact gcgtatgatg agagcaccga tgagaacgtg atgctgctca cgtcagatgc 5701 cccagagtac aaaccctggg ctctggtgat tcaggactct aatggagaga acaagatcaa 5761 gatgctataa tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc 5821 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 5881 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 5941 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 6001 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgatacc 6061 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 6121 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctaggg 6181 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 6241 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 6301 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 6361 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 6421 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 6481 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 6541 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 6601 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 6661 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 6721 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 6781 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 6841 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 6901 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 6961 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7021 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7081 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7141 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7201 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7261 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7321 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7381 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7441 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7501 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 7561 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 7621 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 7681 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 7741 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 7801 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 7861 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 7921 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 7981 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8041 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8101 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8161 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8221 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc 8281 cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct 8341 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag 8401 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct 8461 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta 8521 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac 8581 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac 8641 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt 8701 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat 8761 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga 8821 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt 8881 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca 8941 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg 9001 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta 9061 tataagcaga gctggtttag tgaaccgtca gatccgctag agatccgcgg ccgctaatac 9121 gactcactat agggagagcc gccaccatgt ccgaagtcga gttttcccat gagtactgga 9181 tgagacacgc attgactctc gcaaagaggg cttgggatga acgcgaggtg cccgtggggg 9241 cagtactcgt gcataacaat cgcgtaatcg gcgaaggttg gaataggccg atcggacgcc 9301 acgaccccac tgcacatgcg gaaatcatgg cccttcgaca gggagggctt gtgatgcaga 9361 attatcgact tatcgatgcg acgctgtacg tcacgcttga accttgcgta atgtgcgcgg 9421 gagctatgat tcactcccgc attggacgag ttgtattcgg tgcccgcgac gccaagacgg 9481 gtgccgcagg ttcactgatg gacgtgctgc atcacccagg catgaaccac cgggtagaaa 9541 tcacagaagg catattggcg gacgaatgtg cggcgctgtt gtccgacttt tttcgcatgc 9601 ggaggcagga gatcaaggcc cagaaaaaag cacaatcctc tactgactct ggtggttctt 9661 ctggtggttc tagcggcagc gagactcccg ggacctcaga gtccgccaca cccgaaagtt 9721 ctggtggttc ttctggtggt tcttccgaag tcgagttttc ccatgagtac tggatgagac 9781 acgcattgac tctcgcaaag agggctcgag atgaacgcga ggtgcccgtg ggggcagtac 9841 tcgtgctcaa caatcg //