LOCUS Exported 2729 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Homology arms and mEGFP-linker sequence for N-terminus tagging of human SON. ACCESSION . VERSION . KEYWORDS AICSDP-82:SON-mEGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2729) TITLE Allen Institute for Cell Sciences Plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 2729) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2729 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 998..1714 /codon_start=1 /product="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1043..1064) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1304..1323) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1651..1672 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 cccgctcgcc ggaagtcggc ccaccgggac cggcgcggga ccagcgcggc accgcccctc 61 actgccccgc cctacgacgg cttccctgcc ccattggtcc gcgcggccct gggtctccgc 121 ggcgagaatc tagtctgatt ccctcccgcc ctgggcgctg cctgactgct ccccagccga 181 gacacccaag ccttagcacg tttataatgt gacagtggta cggggtgcca cgcagtgttt 241 ccaggagcgc cgggggggag cgccaaaatg ctaaacccat tcttagccaa ttgtgcgccg 301 ctcacgagtc agagctgagc agctaccgcg gaaaaatctc gtgacacgcc tgactcctga 361 tgagactaaa atgcaccggg ctttgaatgc cggtcgctta ggacatccgg cctattgcta 421 aaaaaaaaaa tgaggctggt tatagaacgc actggggaag acacagggca acagcgagtt 481 cctcctctca catgccagtg ggggcggggg ctgaaaaatt ctctcccgga tgcatatccc 541 tgtcgaaact gaacttggcg ccacctgaga gcgcctgaaa gccgtgtgtt aaacaccggg 601 ttgcccttct tgctcttctt cctcttcgcc tttaatctca ggcacttagc aagcctgtta 661 aggctcccaa acactgctgg ggactgacaa cttatgcgga cacggtcgcc tcaagagaga 721 cggctcctgt aatggcgcct gcgtcagaca ctaacatggc ggacatagtc gtgcgcggcg 781 caaacgtcag cacctctacc ccagccgctc agaaacagca gaggagagca gggccgtgct 841 cactgattgg tccctcggtg gagcgggccg gaccgggcat tccctttgga gccaatcagg 901 aaggcgagag accgaattgg ccgctgcgcc tcctcccgag gcatgctggg agcctggagg 961 actagcgagg aggagttgag agaacggagc ggacgccatg gtgagcaagg gcgaggagct 1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg 1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct 1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 1681 cgggatcact ctcggcatgg acgagctgta caagagcggc ctgagaagcg cgacaaacat 1741 cgagcagatc tttaggtctt tcgtcgtcag taaattccgg gaaattcaac aggagctttc 1801 caggtaaacg cctcccagat tcttctgctc ccaacggacc gttaggccct cacctagcct 1861 tctttggcac ctttcttcgg agacgcagtc gttccccggc tcaggccccg ctagggcccc 1921 gcctgggcct gggatccatt ttccgggccc cccccaaccg cccccccagc cgctcccttc 1981 cttttccctc gaggaacttc gccttttccg aggtaaccca agactccctg tttcagaacc 2041 gagttaagat gttcaccctt ctcccctgtt tgggtccccc ctgccgttta gctactcgta 2101 ggcccggttg tccgccaggg acgggcctag ttccttcctc agctcctcct ccgccgccgc 2161 gtatcccctc cccctctccg cctccgcgga ttttgccggc cggtgcctat gaccggaaag 2221 ggtctctaac ggccgtttct tggttaatct taaatcctct ttattattgc gagcttcaga 2281 ccgcttgggg gtctggatct attaataaga ttattcgggg cctagtgtcc tgtagtatct 2341 gtcagccgtt ctgatagtta aatgtttcaa ttaaaacgaa ctcaaactga tgcacttgct 2401 agtttgttaa aatactcgag ataaagatgg aaggggactt tgaaatggtt gcgattaatt 2461 acagcaagtg aaagtggact ttgtgtgtag gtctattatt ttcttgctac ccctgcccca 2521 gttgacacgc tatttttgtc attgctggta gactttacat aatttgcgct tggcttcaca 2581 aaatttgaag gcgaagagat tgttaaagaa atggttgttt acttaggaaa atagtgtgtc 2641 atgttcagcg gttgtctctc gatatttgct tttgtataag atactcattt ctttaaagta 2701 cagaagtagg aaagagatct tgaaacgta //