LOCUS       Exported               10331 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  dCas9 plant transcriptional activator (AT-CBF1) linked constitutive 
            expression cassette for Zea mays.
ACCESSION   .
VERSION     .
KEYWORDS    PV225
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Joshua K. Young, Stephen L. Gasior, Spencer Jones, Lijuan Wang, 
  TITLE     Repurposing of Type I-E CRISPR-Cascade for gene activation in plants
  JOURNAL   Nature Communications Biology
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             801..821
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1197..1385
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     RBS             4571..4579
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(5856..5874)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5857..5874)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5892..5908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5892..5908)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5905..5927)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5916..5932
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5940..5970)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5985..6006
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6123..6140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6294..6882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6374..6393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7056..7075)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7067..7882
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7140..7159)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8176..8194
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8232..8254)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8354..8373
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8440..8895)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8577..8598)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8789..8808
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9021..9043
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9035..9052
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9036..9052
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9059..9078
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9059..9077
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct
       61 tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt
      121 tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt
      181 ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata
      241 ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt
      301 tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat
      361 gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact
      421 acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg
      481 agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac
      541 tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct
      601 atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat
      661 ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg
      721 cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcaggtcg
      781 actctagagg atccatggca ccgaagaaga agcgcaaggt gatggacaag aagtacagca
      841 tcggcctcgc catcggcacc aactcggtgg gctgggccgt catcacggac gaatataagg
      901 tcccgtcgaa gaagttcaag gtcctcggca atacagaccg ccacagcatc aagaaaaact
      961 tgatcggcgc cctcctgttc gatagcggcg agaccgcgga ggcgaccagg ctcaagagga
     1021 ccgccaggag acggtacact aggcgcaaga acaggatctg ctacctgcag gagatcttca
     1081 gcaacgagat ggcgaaggtg gacgactcct tcttccaccg cctggaggaa tcattcctgg
     1141 tggaggagga caagaagcat gagcggcacc caatcttcgg caacatcgtc gacgaggtaa
     1201 gtttctgctt ctacctttga tatatatata ataattatca ttaattagta gtaatataat
     1261 atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct tttctgtagt
     1321 ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa catggtgatg
     1381 tgcaggtggc ctaccacgag aagtacccga caatctacca cctccggaag aaactggtgg
     1441 acagcacaga caaggcggac ctccggctca tctaccttgc cctcgcgcat atgatcaagt
     1501 tccgcggcca cttcctcatc gagggcgacc tgaacccgga caactccgac gtggacaagc
     1561 tgttcatcca gctcgtgcag acgtacaatc aactgttcga ggagaacccc ataaacgcta
     1621 gcggcgtgga cgccaaggcc atcctctcgg ccaggctctc gaaatcaaga aggctggaga
     1681 accttatcgc gcagttgcca ggcgaaaaga agaacggcct cttcggcaac cttattgcgc
     1741 tcagcctcgg cctgacgccg aacttcaaat caaacttcga cctcgcggag gacgccaagc
     1801 tccagctctc aaaggacacc tacgacgacg acctcgacaa cctcctggcc cagataggag
     1861 accagtacgc ggacctcttc ctcgccgcca agaacctctc cgacgctatc ctgctcagcg
     1921 acatccttcg ggtcaacacc gaaattacca aggcaccgct gtccgccagc atgattaaac
     1981 gctacgacga gcaccatcag gacctcacgc tgctcaaggc actcgtccgc cagcagctcc
     2041 ccgagaagta caaggagatc ttcttcgacc aatcaaaaaa cggctacgcg ggatatatcg
     2101 acggcggtgc cagccaggaa gagttctaca agttcatcaa accaatcctg gagaagatgg
     2161 acggcaccga ggagttgctg gtcaagctca acagggagga cctcctcagg aagcagagga
     2221 ccttcgacaa cggctccatc ccgcatcaga tccacctggg cgaactgcat gccatcctgc
     2281 ggcgccagga ggacttctac ccgttcctga aggataaccg ggagaagatc gagaagatct
     2341 tgacgttccg catcccatac tacgtgggcc cgctggctcg cggcaactcc cggttcgcct
     2401 ggatgacccg gaagtcggag gagaccatca caccctggaa ctttgaggag gtggtcgata
     2461 agggcgctag cgctcagagc ttcatcgagc gcatgaccaa cttcgataaa aacctgccca
     2521 atgaaaaagt cctccccaag cactcgctgc tctacgagta cttcaccgtg tacaacgagc
     2581 tcaccaaggt caaatacgtc accgagggca tgcggaagcc ggcgttcctg agcggcgagc
     2641 agaagaaggc gatagtggac ctcctcttca agaccaacag gaaggtgacc gtgaagcaat
     2701 taaaagagga ctacttcaag aaaatagagt gcttcgactc cgtggagatc tcgggcgtgg
     2761 aggatcggtt caacgcctca ctcggcacgt atcacgacct cctcaagatc attaaagaca
     2821 aggacttcct cgacaacgag gagaacgagg acatcctcga ggacatcgtc ctcaccctga
     2881 ccctgttcga ggaccgcgaa atgatcgagg agaggctgaa gacctacgcg cacctgttcg
     2941 acgacaaggt catgaaacag ctcaagaggc gccgctacac tggttgggga aggctgtccc
     3001 gcaagctcat taatggcatc agggacaagc agagcggcaa gaccatcctg gacttcctca
     3061 agtccgacgg gttcgccaac cgcaacttca tgcagctcat tcacgacgac tcgctcacgt
     3121 tcaaggaaga catccagaag gcacaggtga gcgggcaggg tgactccctc cacgaacaca
     3181 tcgccaacct ggccggctcg ccggccatta aaaagggcat cctgcagacg gtcaaggtcg
     3241 tcgacgagct cgtgaaggtg atgggccggc acaagcccga aaatatcgtc atagagatgg
     3301 ccagggagaa ccagaccacc caaaaagggc agaagaactc gcgcgagcgg atgaaacgga
     3361 tcgaggaggg cattaaagag ctcgggtccc agatcctgaa ggagcacccc gtggaaaata
     3421 cccagctcca gaatgaaaag ctctacctct actacctgca gaacggccgc gacatgtacg
     3481 tggaccagga gctggacatt aatcggctat cggactacga cgtcgacgcc atcgtgccgc
     3541 agtcgttcct caaggacgat agcatcgaca acaaggtgct cacccggtcg gataaaaatc
     3601 ggggcaagag cgacaacgtg cccagcgagg aggtcgtgaa gaagatgaaa aactactggc
     3661 gccagctcct caacgcgaaa ctgatcaccc agcgcaagtt cgacaacctg acgaaggcgg
     3721 aacgcggtgg cttgagcgaa ctcgataagg cgggcttcat aaaaaggcag ctggtcgaga
     3781 cgcgccagat cacgaagcat gtcgcccaga tcctggacag ccgcatgaat actaagtacg
     3841 atgaaaacga caagctgatc cgggaggtga aggtgatcac gctgaagtcc aagctcgtgt
     3901 cggacttccg caaggacttc cagttctaca aggtccgcga gatcaacaac taccaccacg
     3961 cccacgacgc ctacctgaat gcggtggtcg ggaccgccct gatcaagaag tacccgaagc
     4021 tggagtcgga gttcgtgtac ggcgactaca aggtctacga cgtgcgcaaa atgatcgcca
     4081 agtccgagca ggagatcggc aaggccacgg caaaatactt cttctactcg aacatcatga
     4141 acttcttcaa gaccgagatc accctcgcga acggcgagat ccgcaagcgc ccgctcatcg
     4201 aaaccaacgg cgagacgggc gagatcgtct gggataaggg ccgggatttc gcgacggtcc
     4261 gcaaggtgct ctccatgccg caagtcaata tcgtgaaaaa gacggaggtc cagacgggcg
     4321 ggttcagcaa ggagtccatc ctcccgaagc gcaactccga caagctcatc gcgaggaaga
     4381 aggattggga cccgaaaaaa tatggcggct tcgacagccc gaccgtcgca tacagcgtcc
     4441 tcgtcgtggc gaaggtggag aagggcaagt caaagaagct caagtccgtg aaggagctgc
     4501 tcgggatcac gattatggag cggtcctcct tcgagaagaa cccgatcgac ttcctagagg
     4561 ccaagggata taaggaggtc aagaaggacc tgattattaa actgccgaag tactcgctct
     4621 tcgagctgga aaacggccgc aagaggatgc tcgcctccgc aggcgagttg cagaagggca
     4681 acgagctcgc cctcccgagc aaatacgtca atttcctgta cctcgctagc cactatgaaa
     4741 agctcaaggg cagcccggag gacaacgagc agaagcagct cttcgtggag cagcacaagc
     4801 attacctgga cgagatcatc gagcagatca gcgagttctc gaagcgggtg atcctcgccg
     4861 acgcgaacct ggacaaggtg ctgtcggcat ataacaagca ccgcgacaaa ccaatacgcg
     4921 agcaggccga aaatatcatc cacctcttca ccctcaccaa cctcggcgct ccggcagcct
     4981 tcaagtactt cgacaccacg attgaccgga agcggtacac gagcacgaag gaggtgctcg
     5041 atgcgacgct gatccaccag agcatcacag ggctctatga aacacgcatc gacctgagcc
     5101 agctgggcgg agacaagaga ccacgggacc gccacgatgg cgagctggga ggccgcaagc
     5161 gggcaagggg cagggctacg tgcgccaagg acatccaaaa agcagccgcg gaggcggcac
     5221 tcgcctttca ggacgagacc tgcgacacca ccaccaccaa ccacggcctc gacatggagg
     5281 agaccatggt cgaagccatc tacacccccg agcagagcga gggcgccttc tacatggacg
     5341 aagagacaat gttcggcatg ccaaccctcc tcgacaacat ggccgaaggc atgctgctgc
     5401 cgcccccgag cgtccagtgg aaccacaact acgacggcga aggcgacggg gatgtgtccc
     5461 tgtggagcta ctaggtaccg ttaacctaga cttgtccatc ttctggattg gccaacttaa
     5521 ttaatgtatg aaataaaagg atgcacacat agtgacatgc taatcactat aatgtgggca
     5581 tcaaagttgt gtgttatgtg taattactag ttatctgaat aaaagagaaa gagatcatcc
     5641 atatttctta tcctaaatga atgtcacgtg tctttataat tctttgatga accagatgca
     5701 tttcattaac caaatccata tacatataaa tattaatcat atataattaa tatcaattgg
     5761 gttagcaaaa caaatctagt ctaggtgtgt tttgcgaatg cggccgccac cgcggtggag
     5821 ctcgaattcc ggtccgggtc acccagcttg agtattctat agtgtcacct aaatagcttg
     5881 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5941 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6001 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6061 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6121 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6181 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6241 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6301 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6361 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6421 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6481 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6541 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6601 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6661 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6721 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6781 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6841 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6901 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatggag
     6961 ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     7021 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     7081 acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     7141 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
     7201 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     7261 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     7321 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
     7381 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     7441 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     7501 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     7561 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     7621 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     7681 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     7741 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     7801 acggcttttt cagaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     7861 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     7921 tacgctgact tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag
     7981 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     8041 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
     8101 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgagccatg
     8161 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     8221 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     8281 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     8341 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     8401 ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt
     8461 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     8521 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     8581 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     8641 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     8701 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     8761 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     8821 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     8881 cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     8941 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     9001 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta
     9061 atacgactca ctatagggcg aattgggtta cccggaccga agcttgcatg cctgcagtgc
     9121 agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct aagttataaa
     9181 aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct atctttatac
     9241 atatatttaa actttactct acgaataata taatctatag tactacaata atatcagtgt
     9301 tttagagaat catataaatg aacagttaga catggtctaa aggacaattg agtattttga
     9361 caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt tttttgcaaa
     9421 tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt
     9481 taatggtttt tatagactaa tttttttagt acatctattt tattctattt tagcctctaa
     9541 attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga
     9601 ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga
     9661 aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa
     9721 cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc
     9781 atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc
     9841 gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg
     9901 cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg
     9961 ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac
    10021 ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc
    10081 acctccgctt caaggtacgc cgctcgtcct cccccccccc cctctctacc ttctctagat
    10141 cggcgttccg gtccatgcat ggttagggcc cggtagttct acttctgttc atgtttgtgt
    10201 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
    10261 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
    10321 tctagccgtt c
//
