LOCUS       Exported                7004 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    131_pAAV-ProB13-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      1448..1457
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1454..2377
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             2384..3100
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2429..2450)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2690..2709)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3037..3058
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3131..3719
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3184..3204)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3602..3613)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3751..4227
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3950..3969)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4267..4407
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4267..4396
                     /label=AAV2 ITR
     rep_origin      4482..4937
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4569..4588)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4779..4800
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4954..4973)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5073..5095
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5133..5151)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5219..5323
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5324..6184
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5542..5561)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6355..6943
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6844..6863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgcaggg ctataaagcc ccctccccca ggccagtggg
      181 aggagctggg tggggcatcc ccctccccac acagcggggc cacccaccta tacaaggctg
      241 agtcacgtca gaaccacgga cagcgagctg gcgccaatca ccagcccttt accgtgccct
      301 ggggacccac ttccttcggt gtcacggtcc attagctgtc tttaagacaa gctgaattcc
      361 agaagttcct tgagcagcag catcttcaga cgcagcctga actcaaaatt ctatcctaat
      421 cagctcttct tgttaaggtt cggacagctt attttgttaa ctgcatatat aaaaacgagc
      481 agaaggccag tcacctctgt tggtggggga ggagactttg gcacccagac cctccccttc
      541 ctacttcagg aatgggtacc tggaaaacca tcaaaataat tggacttctt aaaaattgga
      601 ttgtgtgagt gaaaggtgtt tatcagaagc ggatgactcc ggatcttatc atcctggagg
      661 actgcacaga atagttaata tgttccttga gggactagga tgctgacgtc ttttactgat
      721 accggatcat tacgtgactg ggggagaaaa aaaaggaagt catatcatga ataaaaatcg
      781 gagtgcaaca gtgcaaccaa aatattctgt acttgaaggc agaaagatgt tgacaaagag
      841 gcacacaaag gtcagacacc agcctctctg tcttaaatct agaggtagca gacacatggc
      901 tctcagcaaa cccaaagggc ttcagcatcc tttctcctgc agccagccag actcattaga
      961 tatttactga aaatccttat aattctttcc tttaaaacac aaaacgactt ccatgtttag
     1021 tagcctattt gaaaaagcat atgcaaggaa ttgagagatc aaaattaaaa ttattaatag
     1081 gagatcttga gcagggagcc atcctcctag acagactctg ctctgtggac agcaccacag
     1141 gggcgttggc tctgacccat ctcggagggt tcagcactga aaattctcat ccccctagca
     1201 accaaactag atccatcaaa gagcaggacc tggcagccga gctgggagag actaatagcc
     1261 tggaaggaag gcggggcctg gaggtgtgaa tcttaaattt tcaacttgtg cagggaaaag
     1321 ggttcctcat tttattttta gttaaaatga atgtgatctt tttcctagtg gaccagaaaa
     1381 gaaaccaaat cacagtatag gcattattta aacttaggct tggaaaaagc tgagcatgtg
     1441 tggatccgcc accatggatt atggaggcgc cctgagtgcc gttgggcgcg agctgctatt
     1501 tgtaacgaac ccagtagtcg tcaatggctc tgtacttgtg cctgaggacc agtgttactg
     1561 cgcgggctgg attgagtcgc gtggcacaaa cggtgcccaa acggcgtcga acgtgctgca
     1621 atggcttgct gctggcttct ccatcctact gcttatgttt tacgcctacc aaacatggaa
     1681 gtcaacctgc ggctgggagg agatctatgt gtgcgctatc gagatggtca aggtgattct
     1741 cgagttcttc ttcgagttta agaacccgtc catgctgtat ctagccacag gccaccgcgt
     1801 ccagtggttg cgttacgccg agtggcttct cacctgcccg gtcatttgca ttcacctgtc
     1861 aaacctgacg ggcttgtcca acgactacag caggcgcacc atgggtctgc ttgtgtctga
     1921 tattggcaca attgtgtggg gcgccacttc cgccatggcc accggatacg tcaaggtcat
     1981 cttcttctgc ctgggtctgt gttatggtgc taacacgttc tttcacgctg ccaaggccta
     2041 catcgagggt taccacaccg tgccgaaggg ccggtgtcgc caggtggtga ctggcatggc
     2101 ttggctcttc ttcgtatcat ggggtatgtt ccccatcctg ttcatcctcg gccccgaggg
     2161 cttcggcgtc ctgagcgtgt acggctccac cgtcggccac accatcattg acctgatgtc
     2221 gaagaactgc tggggtctgc tcggccacta cctgcgcgtg ctgatccacg agcatatcct
     2281 catccacggc gacattcgca agaccaccaa attgaacatt ggtggcactg agattgaggt
     2341 cgagacgctg gtggaggacg aggccgaggc tggcgcggta cccatggtga gcaagggcga
     2401 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2461 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2521 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2581 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2641 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2701 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2761 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2821 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2881 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2941 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3001 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3061 cgccgccggg atcactctcg gcatggacga gctgtacaag tgataagaat tcgatatcaa
     3121 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3181 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3241 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3301 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3361 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3421 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3481 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     3541 ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     3601 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     3661 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     3721 tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg
     3781 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     3841 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     3901 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     3961 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     4021 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     4081 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     4141 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     4201 tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc
     4261 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     4321 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     4381 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     4441 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     4501 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     4561 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4621 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4681 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4741 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4801 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     4861 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4921 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4981 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5041 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     5101 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     5161 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5221 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5281 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5341 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5401 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5461 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5521 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5581 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5641 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5701 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5761 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5821 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5881 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5941 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6001 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6061 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6121 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6181 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6241 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6301 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6361 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6421 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6481 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6541 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6601 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6661 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6721 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6781 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6841 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6901 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6961 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
