LOCUS Exported 6671 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 12_pAAV-ProC2-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6671) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6671) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6671 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 912..1108 /label=SV40 promoter /note="SV40 early promoter" rep_origin 959..1094 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1021..1040 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 1115..1124 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1121..2044 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 2051..2767 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2096..2117) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2357..2376) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2704..2725 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2798..3386 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2851..2871) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3269..3280) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3418..3894 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3617..3636) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3934..4074 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3934..4063 /label=AAV2 ITR rep_origin 4149..4604 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4236..4255) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4446..4467 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4621..4640) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4740..4762 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4800..4818) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4886..4990 /gene="bla" /label=AmpR promoter CDS 4991..5851 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5209..5228) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6022..6610 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6511..6530 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgttcaagc ctcctaccgc ctttgttatg caaacatatc 181 aaacgccctc ctttgttatg caaaagggct ggaacggggc ctttgttatg caaatcgccc 241 tccccgatcc ctttgttatg caaatttgac gaattcccac ctttgttatg caaacaaatc 301 tcctaccctc ctttgttatg caaagtgaga ggggctgcac ctttgttatg caaaatgcgg 361 cccctgagac ctttgttatg caaaaaccat gtacgcttgc ctttgttatg caaaccgcct 421 gttgcttggc ctttgttatg caaagccacg cgattggcgc ctttgttatg caaagctcgg 481 ttatgtacac ctttgttatg caaagctact ttaaacttgc ctttgttatg caaatcacga 541 cctgaccgtc ctttgttatg caaaaacggt tgaaatagtc ctttgttatg caaaatgata 601 ttgaatagtc ctttgttatg caaaaattta gatgccgacc ctttgttatg caaaggaatg 661 ggcgtgctgc ctttgttatg caaattttcg ctgcgacagc ctttgttatg caaacatgct 721 cgccactcac ctttgttatg caaaggtcta acaatgaccc ctttgttatg caaactacgt 781 ggaatagatc ctttgttatg caaaccccga gtttttgaac ctttgttatg caaaatcagt 841 aacttcattc ctttgttatg caaaatgtga cttaacctcc ctttgttatg caaagctcga 901 gatctgcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 961 cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac taattttttt 1021 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 1081 cttttttgga ggcctaggct tttgcaaagg atccgccacc atggattatg gaggcgccct 1141 gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca gtagtcgtca atggctctgt 1201 acttgtgcct gaggaccagt gttactgcgc gggctggatt gagtcgcgtg gcacaaacgg 1261 tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct ggcttctcca tcctactgct 1321 tatgttttac gcctaccaaa catggaagtc aacctgcggc tgggaggaga tctatgtgtg 1381 cgctatcgag atggtcaagg tgattctcga gttcttcttc gagtttaaga acccgtccat 1441 gctgtatcta gccacaggcc accgcgtcca gtggttgcgt tacgccgagt ggcttctcac 1501 ctgcccggtc atttgcattc acctgtcaaa cctgacgggc ttgtccaacg actacagcag 1561 gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt gtgtggggcg ccacttccgc 1621 catggccacc ggatacgtca aggtcatctt cttctgcctg ggtctgtgtt atggtgctaa 1681 cacgttcttt cacgctgcca aggcctacat cgagggttac cacaccgtgc cgaagggccg 1741 gtgtcgccag gtggtgactg gcatggcttg gctcttcttc gtatcatggg gtatgttccc 1801 catcctgttc atcctcggcc ccgagggctt cggcgtcctg agcgtgtacg gctccaccgt 1861 cggccacacc atcattgacc tgatgtcgaa gaactgctgg ggtctgctcg gccactacct 1921 gcgcgtgctg atccacgagc atatcctcat ccacggcgac attcgcaaga ccaccaaatt 1981 gaacattggt ggcactgaga ttgaggtcga gacgctggtg gaggacgagg ccgaggctgg 2041 cgcggtaccc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 2101 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 2161 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 2221 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 2281 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 2341 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 2401 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 2461 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 2521 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 2581 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 2641 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 2701 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 2761 gtacaagtga taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat 2821 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 2881 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 2941 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 3001 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 3061 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 3121 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 3181 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct 3241 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 3301 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 3361 gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg 3421 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg 3481 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc 3541 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc 3601 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc 3661 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg 3721 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt 3781 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg 3841 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt 3901 ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca 3961 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc 4021 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc 4081 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca 4141 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 4201 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt 4261 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 4321 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg 4381 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 4441 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt 4501 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca 4561 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag 4621 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga 4681 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 4741 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg 4801 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 4861 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 4921 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 4981 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 5041 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 5101 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 5161 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 5221 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 5281 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 5341 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 5401 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 5461 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 5521 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 5581 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 5641 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 5701 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 5761 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 5821 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 5881 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 5941 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 6001 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 6061 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 6121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 6181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 6241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 6301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 6361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 6421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 6481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 6541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 6601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 6661 tgctcacatg t //