LOCUS Exported 6471 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 9_pAAV-ProC5-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6471) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6471) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6471 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 712..908 /label=SV40 promoter /note="SV40 early promoter" rep_origin 759..894 /label=SV40 ori /note="SV40 origin of replication" primer_bind 821..840 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 915..924 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 921..1844 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1851..2567 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1896..1917) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2157..2176) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2504..2525 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2598..3186 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2651..2671) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3069..3080) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3218..3694 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3417..3436) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3734..3874 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3734..3863 /label=AAV2 ITR rep_origin 3949..4404 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4036..4055) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4246..4267 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4421..4440) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4540..4562 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4600..4618) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4686..4790 /gene="bla" /label=AmpR promoter CDS 4791..5651 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5009..5028) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5822..6410 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6311..6330 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtggcata agggggaacc aagcttaaac agtcccatgg 181 ccaagcttct tattcgtggc gcacaagctt ggcgatggcc aaagccaagc ttactgagtg 241 caactgtcaa gcttcactat cagcgatttc aagcttaaac aattgactgt acaagcttcc 301 catattacgc tagcaagctt gaaacggtac agagccaagc ttatttatct cgctaaccaa 361 gctttcctac ttacacgtac aagcttgagg tctaaaaact gcaagctttc gaagtaccag 421 acccaagctt ggcaactcac tgaaccaagc ttgagcgact gtcggtacaa gcttaagcgg 481 gctcgtccac aagctttgaa cggggatcgg gcaagcttgt cacattaggc ggccaagctt 541 caatccactg gaatacaagc ttcctctggt tctaactcaa gcttcttaga cgtgtgaagc 601 aagctttaaa taatcagaac ccaagcttaa gagataaaga agacaagctt agtcacgctg 661 ggctccaagc ttaatgatgg atgagctcaa gcttgctcga gatctgcgat ctgcatctca 721 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 781 gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg 841 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 901 tttgcaaagg atccgccacc atggattatg gaggcgccct gagtgccgtt gggcgcgagc 961 tgctatttgt aacgaaccca gtagtcgtca atggctctgt acttgtgcct gaggaccagt 1021 gttactgcgc gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg 1081 tgctgcaatg gcttgctgct ggcttctcca tcctactgct tatgttttac gcctaccaaa 1141 catggaagtc aacctgcggc tgggaggaga tctatgtgtg cgctatcgag atggtcaagg 1201 tgattctcga gttcttcttc gagtttaaga acccgtccat gctgtatcta gccacaggcc 1261 accgcgtcca gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc atttgcattc 1321 acctgtcaaa cctgacgggc ttgtccaacg actacagcag gcgcaccatg ggtctgcttg 1381 tgtctgatat tggcacaatt gtgtggggcg ccacttccgc catggccacc ggatacgtca 1441 aggtcatctt cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca 1501 aggcctacat cgagggttac cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg 1561 gcatggcttg gctcttcttc gtatcatggg gtatgttccc catcctgttc atcctcggcc 1621 ccgagggctt cggcgtcctg agcgtgtacg gctccaccgt cggccacacc atcattgacc 1681 tgatgtcgaa gaactgctgg ggtctgctcg gccactacct gcgcgtgctg atccacgagc 1741 atatcctcat ccacggcgac attcgcaaga ccaccaaatt gaacattggt ggcactgaga 1801 ttgaggtcga gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc atggtgagca 1861 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 1921 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 1981 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 2041 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 2101 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 2161 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 2221 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 2281 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 2341 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 2401 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 2461 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 2521 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga taagaattcg 2581 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta 2641 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 2701 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 2761 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 2821 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 2881 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 2941 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt 3001 cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct 3061 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 3121 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 3181 ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc 3241 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat 3301 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag 3361 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta 3421 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg 3481 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac 3541 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct 3601 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt 3661 acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg 3721 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 3781 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc 3841 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt 3901 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta 3961 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 4021 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 4081 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 4141 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat 4201 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 4261 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc 4321 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 4381 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg 4441 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 4501 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 4561 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt 4621 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa 4681 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 4741 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 4801 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 4861 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 4921 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 4981 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 5041 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 5101 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 5161 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 5221 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 5281 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 5341 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 5401 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 5461 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 5521 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 5581 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 5641 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 5701 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 5761 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 5821 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 5881 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 5941 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 6001 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 6061 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 6121 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 6181 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 6241 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 6301 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 6361 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 6421 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t //