LOCUS Exported 6481 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 151_pAAV-ProC29-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6481) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6481) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6481 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 722..918 /label=SV40 promoter /note="SV40 early promoter" rep_origin 769..904 /label=SV40 ori /note="SV40 origin of replication" primer_bind 831..850 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 925..934 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 931..1854 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1861..2577 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1906..1927) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2167..2186) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2514..2535 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2608..3196 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2661..2681) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3079..3090) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3228..3704 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3427..3446) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3744..3884 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3744..3873 /label=AAV2 ITR rep_origin 3959..4414 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4046..4065) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4256..4277 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4431..4450) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4550..4572 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4610..4628) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4696..4800 /gene="bla" /label=AmpR promoter CDS 4801..5661 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5019..5038) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5832..6420 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6321..6340 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtcttacc gattgcagac gaaaccgaaa cttagctgac 181 atcgagtcga aaccgaaact tttcatggcc gaaggcgaaa ccgaaacttg cgtccgtgta 241 acgcgaaacc gaaacttccc gagcatcctt acgaaaccga aactgcaacc ggctacactc 301 gaaaccgaaa ctactaatga taccaaccga aaccgaaact cggtaagtga aactgcgaaa 361 ccgaaacttg gtggacagcc ttccgaaacc gaaactggac gatgttcgtt ccgaaaccga 421 aactatattc gtgcccaagc gaaaccgaaa ctagggggcc gatatcccga aaccgaaact 481 acaggccccg cccgtcgaaa ccgaaactta cgtctcagga agtcgaaacc gaaactggct 541 accactgacc acgaaaccga aacttctact gttccgtgac gaaaccgaaa ctgacggaca 601 agagtaccga aaccgaaact tagtcaggcg tccatcgaaa ccgaaactca aagattcaaa 661 aaacgaaacc gaaactttct gttgggatgt ccgaaaccga aactgctcga gatctgcgat 721 ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 781 actccgccca gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca 841 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 901 ggcctaggct tttgcaaagg atccgccacc atggattatg gaggcgccct gagtgccgtt 961 gggcgcgagc tgctatttgt aacgaaccca gtagtcgtca atggctctgt acttgtgcct 1021 gaggaccagt gttactgcgc gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg 1081 gcgtcgaacg tgctgcaatg gcttgctgct ggcttctcca tcctactgct tatgttttac 1141 gcctaccaaa catggaagtc aacctgcggc tgggaggaga tctatgtgtg cgctatcgag 1201 atggtcaagg tgattctcga gttcttcttc gagtttaaga acccgtccat gctgtatcta 1261 gccacaggcc accgcgtcca gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc 1321 atttgcattc acctgtcaaa cctgacgggc ttgtccaacg actacagcag gcgcaccatg 1381 ggtctgcttg tgtctgatat tggcacaatt gtgtggggcg ccacttccgc catggccacc 1441 ggatacgtca aggtcatctt cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt 1501 cacgctgcca aggcctacat cgagggttac cacaccgtgc cgaagggccg gtgtcgccag 1561 gtggtgactg gcatggcttg gctcttcttc gtatcatggg gtatgttccc catcctgttc 1621 atcctcggcc ccgagggctt cggcgtcctg agcgtgtacg gctccaccgt cggccacacc 1681 atcattgacc tgatgtcgaa gaactgctgg ggtctgctcg gccactacct gcgcgtgctg 1741 atccacgagc atatcctcat ccacggcgac attcgcaaga ccaccaaatt gaacattggt 1801 ggcactgaga ttgaggtcga gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc 1861 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 1921 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 1981 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 2041 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 2101 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 2161 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 2221 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 2281 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 2341 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 2401 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 2461 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 2521 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga 2581 taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga 2641 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 2701 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 2761 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 2821 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 2881 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 2941 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 3001 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg 3061 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 3121 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 3181 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct 3241 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc 3301 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta 3361 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct 3421 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct 3481 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg 3541 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat 3601 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat 3661 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa 3721 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct 3781 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc 3841 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta 3901 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac 3961 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 4021 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 4081 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 4141 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca 4201 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 4261 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa 4321 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac 4381 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc 4441 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga 4501 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc 4561 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata 4621 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 4681 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 4741 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 4801 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 4861 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 4921 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 4981 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 5041 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 5101 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 5161 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 5221 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 5281 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 5341 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 5401 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 5461 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 5521 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 5581 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 5641 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 5701 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 5761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 5821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 5881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 5941 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 6001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 6061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 6121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 6181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 6241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 6301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 6361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 6421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 6481 t //