LOCUS       Exported                 825 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDUET-ctCPR-A17AMO
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD
  TITLE     Engineering Escherichia coli for production of functionalized 
            terpenoids using plant P450s.
  JOURNAL   Nat Chem Biol. 2007 May . 3(5):274-7.
  PUBMED    17438551
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     75..94
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        75..93
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    94..118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             133..155
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cctnnnggng ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatcgatct
       61 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
      121 tctagaaata attttgttta actttaagaa ggagatatac catggcgctg gataaactgg
      181 atctgtatgt gatcattacg ctggtagttg cggttgctgc ttatttcgcg aagaaccagt
      241 ttctggacca gccgcaggac accggcttcc tgaacaccga ttctggcagc aactctcgtg
      301 acgtactgtc taccctgaag aagaacaaca agaacactct gctgctgttc ggcagccaga
      361 ctggcaccgc tgaggattac gcgaacaaac tgtctcgtga actgcattcc cgcttcggtc
      421 tgaagactat ggttgcggat ttcgcagact acgactggga caatttcggc gacatcaccg
      481 aagacatcct ggtgttcttt atcgtggcca cgtacggcga aggcgaaccg accgataacg
      541 cagatgaatt tcacacgtgg ctgacggagg aggctgacac cctgtccacc ctgaagtaca
      601 ccgttttcgg tctgggtaac agcacctatg agttcttcaa cgctatcggt cgtaaatttg
      661 accgtctgct gtctgaaaag ggtggtgatc gtttcgcgga atatgctgaa ggcgatgacg
      721 gtactggtac tctggatgaa gattcatggc ttgaaagaca cgttttcgat gcactgaaga
      781 cnnanctgat ttcgaggaga aagnnctgaa gtacgaccga cgnna
//
