LOCUS Exported 9781 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript. ACCESSION . VERSION . KEYWORDS pCE046-SiT-Cas12a-[Repr] SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9781) AUTHORS Campa CC, Weisbach NR, Santinha AJ, Incarnato D, Platt RJ TITLE Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts. JOURNAL Nat Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 10.1038/s41592-019-0508-6. PUBMED 31406383 REFERENCE 2 (bases 1 to 9781) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9781 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 233..1411 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 464..1402 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1359..1379 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 1446..5366 /codon_start=1 /product="CRISPR-associated protein from Acidaminococcus sp. BV3L6 (Zetsche et al., 2015)" /label=AsCpf1 /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES KDLKLQNGISNQDWLAYIQELRN" regulatory 5086..5095 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 5367..5414 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 5427..5612 /codon_start=1 /product="Kruppel-associated box (KRAB) transcriptional repression domain from the human zinc finger protein ZNF10 (Margolin et al., 1994)" /label=KRAB /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY QLTKPDVILRLEKGEEP" CDS 5625..5810 /codon_start=1 /product="Kruppel-associated box (KRAB) transcriptional repression domain from the human zinc finger protein ZNF10 (Margolin et al., 1994)" /label=KRAB /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY QLTKPDVILRLEKGEEP" CDS 5823..6008 /codon_start=1 /product="Kruppel-associated box (KRAB) transcriptional repression domain from the human zinc finger protein ZNF10 (Margolin et al., 1994)" /label=KRAB /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY QLTKPDVILRLEKGEEP" CDS 6015..6095 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA" primer_bind 6292..6308 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6292..6308 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" misc_feature 6369..6957 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6422..6442) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6840..6851) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" repeat_region 7044..7184 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 7044..7173 /label=AAV2 ITR rep_origin 7259..7714 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7346..7365) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7556..7577 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7731..7750) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7850..7872 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7910..7928) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7996..8100 /gene="bla" /label=AmpR promoter CDS 8101..8961 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8319..8338) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9132..9720 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9621..9640 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtttaatt aatgcaaaga tggataaagt tttaaacaga 181 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 241 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 301 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 361 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 421 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 481 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 541 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 601 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 661 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 721 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 781 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 841 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 901 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 961 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 1021 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 1081 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 1141 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 1201 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 1261 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 1321 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 1381 aagttttttt cttccatttc aggtgtcgtg agctagcgtt taaacttaag cttggtaccg 1441 ccaccatgac acagttcgag ggctttacca acctgtatca ggtgagcaag acactgcggt 1501 ttgagctgat cccacagggc aagaccctga agcacatcca ggagcagggc ttcatcgagg 1561 aggacaaggc ccgcaatgat cactacaagg agctgaagcc catcatcgat cggatctaca 1621 agacctatgc cgaccagtgc ctgcagctgg tgcagctgga ttgggagaac ctgagcgccg 1681 ccatcgactc ctatagaaag gagaaaaccg aggagacaag gaacgccctg atcgaggagc 1741 aggccacata tcgcaatgcc atccacgact acttcatcgg ccggacagac aacctgaccg 1801 atgccatcaa taagagacac gccgagatct acaagggcct gttcaaggcc gagctgttta 1861 atggcaaggt gctgaagcag ctgggcaccg tgaccacaac cgagcacgag aacgccctgc 1921 tgcggagctt cgacaagttt acaacctact tctccggctt ttatgagaac aggaagaacg 1981 tgttcagcgc cgaggatatc agcacagcca tcccacaccg catcgtgcag gacaacttcc 2041 ccaagtttaa ggagaattgt cacatcttca cacgcctgat caccgccgtg cccagcctgc 2101 gggagcactt tgagaacgtg aagaaggcca tcggcatctt cgtgagcacc tccatcgagg 2161 aggtgttttc cttccctttt tataaccagc tgctgacaca gacccagatc gacctgtata 2221 accagctgct gggaggaatc tctcgggagg caggcaccga gaagatcaag ggcctgaacg 2281 aggtgctgaa tctggccatc cagaagaatg atgagacagc ccacatcatc gcctccctgc 2341 cacacagatt catccccctg tttaagcaga tcctgtccga taggaacacc ctgtctttca 2401 tcctggagga gtttaagagc gacgaggaag tgatccagtc cttctgcaag tacaagacac 2461 tgctgagaaa cgagaacgtg ctggagacag ccgaggccct gtttaacgag ctgaacagca 2521 tcgacctgac acacatcttc atcagccaca agaagctgga gacaatcagc agcgccctgt 2581 gcgaccactg ggatacactg aggaatgccc tgtatgagcg gagaatctcc gagctgacag 2641 gcaagatcac caagtctgcc aaggagaagg tgcagcgcag cctgaagcac gaggatatca 2701 acctgcagga gatcatctct gccgcaggca aggagctgag cgaggccttc aagcagaaaa 2761 ccagcgagat cctgtcccac gcacacgccg ccctggatca gccactgcct acaaccctga 2821 agaagcagga ggagaaggag atcctgaagt ctcagctgga cagcctgctg ggcctgtacc 2881 acctgctgga ctggtttgcc gtggatgagt ccaacgaggt ggaccccgag ttctctgccc 2941 ggctgaccgg catcaagctg gagatggagc cttctctgag cttctacaac aaggccagaa 3001 attatgccac caagaagccc tactccgtgg agaagttcaa gctgaacttt cagatgccta 3061 cactggcctc tggctgggac gtgaataagg agaagaacaa tggcgccatc ctgtttgtga 3121 agaacggcct gtactatctg ggcatcatgc caaagcagaa gggcaggtat aaggccctga 3181 gcttcgagcc cacagagaaa accagcgagg gctttgataa gatgtactat gactacttcc 3241 ctgatgccgc caagatgatc ccaaagtgca gcacccagct gaaggccgtg acagcccact 3301 ttcagaccca cacaaccccc atcctgctgt ccaacaattt catcgagcct ctggagatca 3361 caaaggagat ctacgacctg aacaatcctg agaaggagcc aaagaagttt cagacagcct 3421 acgccaagaa aaccggcgac cagaagggct acagagaggc cctgtgcaag tggatcgact 3481 tcacaaggga ttttctgtcc aagtatacca agacaacctc tatcgatctg tctagcctgc 3541 ggccatcctc tcagtataag gacctgggcg agtactatgc cgagctgaat cccctgctgt 3601 accacatcag cttccagaga atcgccgaga aggagatcat ggatgccgtg gagacaggca 3661 agctgtacct gttccagatc tataacaagg actttgccaa gggccaccac ggcaagccta 3721 atctgcacac actgtattgg accggcctgt tttctccaga gaacctggcc aagacaagca 3781 tcaagctgaa tggccaggcc gagctgttct accgccctaa gtccaggatg aagaggatgg 3841 cacaccggct gggagagaag atgctgaaca agaagctgaa ggatcagaaa accccaatcc 3901 ccgacaccct gtaccaggag ctgtacgact atgtgaatca cagactgtcc cacgacctgt 3961 ctgatgaggc cagggccctg ctgcccaacg tgatcaccaa ggaggtgtct cacgagatca 4021 tcaaggatag gcgctttacc agcgacaagt tctttttcca cgtgcctatc acactgaact 4081 atcaggccgc caattcccca tctaagttca accagagggt gaatgcctac ctgaaggagc 4141 accccgagac acctatcatc ggcatcgatc ggggcgagag aaacctgatc tatatcacag 4201 tgatcgactc caccggcaag atcctggagc agcggagcct gaacaccatc cagcagtttg 4261 attaccagaa gaagctggac aacagggaga aggagagggt ggcagcaagg caggcctggt 4321 ctgtggtggg cacaatcaag gatctgaagc agggctatct gagccaggtc atccacgaga 4381 tcgtggacct gatgatccac taccaggccg tggtggtgct ggagaacctg aatttcggct 4441 ttaagagcaa gaggaccggc atcgccgaga aggccgtgta ccagcagttc gagaagatgc 4501 tgatcgataa gctgaattgc ctggtgctga aggactatcc agcagagaaa gtgggaggcg 4561 tgctgaaccc ataccagctg acagaccagt tcacctcctt tgccaagatg ggcacccagt 4621 ctggcttcct gttttacgtg cctgccccat atacatctaa gatcgatccc ctgaccggct 4681 tcgtggaccc cttcgtgtgg aaaaccatca agaatcacga gagccgcaag cacttcctgg 4741 agggcttcga ctttctgcac tacgacgtga aaaccggcga cttcatcctg cactttaaga 4801 tgaacagaaa tctgtccttc cagaggggcc tgcccggctt tatgcctgca tgggatatcg 4861 tgttcgagaa gaacgagaca cagtttgacg ccaagggcac ccctttcatc gccggcaaga 4921 gaatcgtgcc agtgatcgag aatcacagat tcaccggcag ataccgggac ctgtatcctg 4981 ccaacgagct gatcgccctg ctggaggaga agggcatcgt gttcagggat ggctccaaca 5041 tcctgccaaa gctgctggag aatgacgatt ctcacgccat cgacaccatg gtggccctga 5101 tccgcagcgt gctgcagatg cggaactcca atgccgccac aggcgaggac tatatcaaca 5161 gccccgtgcg cgatctgaat ggcgtgtgct tcgactcccg gtttcagaac ccagagtggc 5221 ccatggacgc cgatgccaat ggcgcctacc acatcgccct gaagggccag ctgctgctga 5281 atcacctgaa ggagagcaag gatctgaagc tgcagaacgg catctccaat caggactggc 5341 tggcctacat ccaggagctg cgcaacaaaa ggccggcggc cacgaaaaag gccggccagg 5401 caaaaaagaa aaagggatcc ggtgggcgga cccttgttac tttcaaggat gtattcgtgg 5461 atttcacacg cgaggagtgg aaattgttgg atactgccca gcagattgta taccgcaatg 5521 taatgctcga aaactacaaa aatctcgtga gtctcggata tcaactgacc aagcctgacg 5581 ttatcctccg attggagaaa ggcgaggaac ctgggagcgg aggaaggacg cttgtcacgt 5641 ttaaggatgt ttttgttgac ttcactcgag aggagtggaa gctgttggat actgcccaac 5701 aaattgtgta tcgcaacgtg atgctcgaga actacaagaa ccttgtgtct ttgggctatc 5761 agctgactaa acctgatgta atactccgcc tcgagaaagg agaggagcct ggtagtgggg 5821 gacggaccct ggtaacattc aaggatgtat tcgtggattt cacgcgggaa gaatggaaac 5881 tcctcgatac tgcccaacag atagtgtatc gaaacgtaat gctcgaaaac tacaaaaact 5941 tggtcagcct cggataccaa cttacaaaac ctgatgttat cctcagactt gagaagggcg 6001 aggaacccgg atcctaccca tacgatgttc cagattacgc ttatccctac gacgtgcctg 6061 attatgcata cccatatgat gtccccgact atgcctaaga attcgattcg tcagtagggt 6121 tgtaaaggtt tttcttttcc tgagaaaaca accttttgtt ttctcaggtt ttgctttttg 6181 gcctttccct agctttaaaa aaaaaaaagc aaaactcacc gaggcagttc cataggatgg 6241 caagatcctg gtattggtct gcgaaatttc tactcttgta gattgaagag ccaggaaaca 6301 gctatgacgc tcttctaatt tctactcttg tagattctag aaaagaattc gatatcaagc 6361 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 6421 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 6481 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 6541 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 6601 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 6661 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 6721 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt 6781 ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 6841 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 6901 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc 6961 gataccgagc gctaataaaa gatctttatt ttcattagat ctgtgtgttg gttttttgtg 7021 tcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct 7081 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc 7141 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta 7201 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac 7261 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 7321 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 7381 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 7441 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca 7501 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 7561 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa 7621 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac 7681 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc 7741 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga 7801 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccttctc cgggagctgc 7861 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga tacgaaaggg cctcgtgata 7921 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 7981 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 8041 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 8101 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 8161 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 8221 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 8281 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 8341 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 8401 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 8461 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 8521 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 8581 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 8641 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 8701 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 8761 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 8821 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 8881 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 8941 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 9001 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 9061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 9121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 9181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 9241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 9301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 9361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 9421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 9481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 9541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 9601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 9661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 9721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 9781 t //