LOCUS       Exported                8965 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    CAG pShuttle
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8965)
  TITLE     pShuttle vectors
REFERENCE   2  (bases 1 to 8965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        411..790
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1050..2066
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1990..2012
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2074..2093
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(2259..2278)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2324..2379
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2378..2397)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   5924..6026
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(6115..6132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6286..6874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6366..6385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7706..8500
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7760..7779)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8370..8389
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(8548..8928)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8610..8631)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8822..8841
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactggtacc gggccccccc
      361 tcgacattga ttattgacta gttattaata gtaatcaatt acsscccctc gacattgatt
      421 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      481 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      541 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      601 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      661 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      721 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      781 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
      841 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
      901 gggggggggg gggggcgcgc gggggggggg gsggggcgrg gcggagaggt gcggcggcag
      961 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     1021 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
     1081 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     1141 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
     1201 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
     1261 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     1321 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     1381 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
     1441 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
     1501 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     1561 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     1621 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     1681 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
     1741 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
     1801 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
     1861 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     1921 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     1981 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     2041 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     2101 atcattttgg caaagaatta gcggccgcgc tactatttaa atgatatcaa gcttcctgca
     2161 gggtcgacgg taccaggccg cactcctcag gtgcaggctg cctatcagaa ggtggtggct
     2221 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
     2281 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     2341 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     2401 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     2461 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     2521 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     2581 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     2641 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     2701 agatccctcg acccctcgag ggcccaagct gtttggggga tctgggcgtg gttaagggtg
     2761 ggaaagaata tataaggtgg gggtcttatg tagttttgta tctgttttgc agcagccgcc
     2821 gccgccatga gcaccaactc gtttgatgga agcattgtga gctcatattt gacaacgcgc
     2881 atgcccccat gggccggggt gcgtcagaat gtgatgggct ccagcattga tggtcgcccc
     2941 gtcctgcccg caaactctac taccttgacc tacgagaccg tgtctggaac gccgttggag
     3001 actgcagcct ccgccgccgc ttcagccgct gcagccaccg cccgcgggat tgtgactgac
     3061 tttgctttcc tgagcccgct tgcaagcagt gcagcttccc gttcatccgc ccgcgatgac
     3121 aagttgacgg ctcttttggc acaattggat tctttgaccc gggaacttaa tgtcgtttct
     3181 cagcagctgt tggatctgcg ccagcaggtt tctgccctga aggcttcctc ccctcccaat
     3241 gcggtttaaa acataaataa aaaaccagac tctgtttgga tttggatcaa gcaagtgtct
     3301 tgctgtcttt atttaggggt tttgcgcgcg cggtaggccc gggaccagcg gtctcggtcg
     3361 ttgagggtcc tgtgtatttt ttccaggacg tggtaaaggt gactctggat gttcagatac
     3421 atgggcataa gcccgtctct ggggtggagg tagcaccact gcagagcttc atgctgcggg
     3481 gtggtgttgt agatgatcca gtcgtagcag gagcgctggg cgtggtgcct aaaaatgtct
     3541 ttcagtagca agctgattgc caggggcagg cccttggtgt aagtgtttac aaagcggtta
     3601 agctgggatg ggtgcatacg tggggatatg agatgcatct tggactgtat ttttaggttg
     3661 gctatgttcc cagccatatc cctccgggga ttcatgttgt gcagaaccac cagcacagtg
     3721 tatccggtgc acttgggaaa tttgtcatgt agcttagaag gaaatgcgtg gaagaacttg
     3781 gagacgccct tgtgacctcc aagattttcc atgcattcgt ccataatgat ggcaatgggc
     3841 ccacgggcgg cggcctgggc gaagatattt ctgggatcac taacgtcata gttgtgttcc
     3901 aggatgagat cgtcataggc catttttaca aagcgcgggc ggagggtgcc agactgcggt
     3961 ataatggttc catccggccc aggggcgtag ttaccctcac agatttgcat ttcccacgct
     4021 ttgagttcag atggggggat catgtctacc tgcggggcga tgaagaaaac ggtttccggg
     4081 gtaggggaga tcagctggga agaaagcagg ttcctgagca gctgcgactt accgcagccg
     4141 gtgggcccgt aaatcacacc tattaccggc tgcaactggt agttaagaga gctgcagctg
     4201 ccgtcatccc tgagcagggg ggccacttcg ttaagcatgt ccctgactcg catgttttcc
     4261 ctgaccaaat ccgccagaag gcgctcgccg cccagcgata gcagttcttg caaggaagca
     4321 aagtttttca acggtttgag accgtccgcc gtaggcatgc ttttgagcgt ttgaccaagc
     4381 agttccaggc ggtcccacag ctcggtcacc tgctctacgg catctcgatc cagcatatct
     4441 cctcgtttcg cgggttgggg cggctttcgc tgtacggcag tagtcggtgc tcgtccagac
     4501 gggccagggt catgtctttc cacgggcgca gggtcctcgt cagcgtagtc tgggtcacgg
     4561 tgaaggggtg cgctccgggc tgcgcgctgg ccagggtgcg cttgaggctg gtcctgctgg
     4621 tgctgaagcg ctgccggtct tcgccctgcg cgtcggccag gtagcatttg accatggtgt
     4681 catagtccag cccctccgcg gcgtggccct tggcgcgcag cttgcccttg gaggaggcgc
     4741 cgcacgaggg gcagtgcaga cttttgaggg cgtagagctt gggcgcgaga aataccgatt
     4801 ccggggagta ggcatccgcg ccgcaggccc cgcagacggt ctcgcattcc acgagccagg
     4861 tgagctctgg ccgttcgggg tcaaaaacca ggtttccccc atgctttttg atgcgtttct
     4921 tacctctggt ttccatgagc cggtgtccac gctcggtgac gaaaaggctg tccgtgtccc
     4981 cgtatacaga cttgagaggg agtttaaacg aattcaatag cttgttgcat gggcggcgat
     5041 ataaaatgca aggtgctgct caaaaaatca ggcaaagcct cgcgcaaaaa agaaagcaca
     5101 tcgtagtcat gctcatgcag ataaaggcag gtaagctccg gaaccaccac agaaaaagac
     5161 accatttttc tctcaaacat gtctgcgggt ttctgcataa acacaaaata aaataacaaa
     5221 aaaacattta aacattagaa gcctgtctta caacaggaaa aacaaccctt ataagcataa
     5281 gacggactac ggccatgccg gcgtgaccgt aaaaaaactg gtcaccgtga ttaaaaagca
     5341 ccaccgacag ctcctcggtc atgtccggag tcataatgta agactcggta aacacatcag
     5401 gttgattcac atcggtcagt gctaaaaagc gaccgaaata gcccggggga atacataccc
     5461 gcaggcgtag agacaacatt acagccccca taggaggtat aacaaaatta ataggagaga
     5521 aaaacacata aacacctgaa aaaccctcct gcctaggcaa aatagcaccc tcccgctcca
     5581 gaacaacata cagcgcttcc acagcggcag ccataacagt cagccttacc agtaaaaaag
     5641 aaaacctatt aaaaaaacac cactcgacac ggcaccagct caatcagtca cagtgtaaaa
     5701 aagggccaag tgcagagcga gtatatatag gactaaaaaa tgacgtaacg gttaaagtcc
     5761 acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca gaaacgaaag ccaaaaaacc
     5821 cacaacttcc tcaaatcgtc acttccgttt tcccacgtta cgtcacttcc cattttaaga
     5881 aaactacaat tcccaacaca tacaagttac tccgccctaa aacctacgtc acccgccccg
     5941 ttcccacgcc ccgcgccacg tcacaaactc caccccctca ttatcatatt ggcttcaatc
     6001 caaaataagg tatattattg atgatgttaa ttaacatgca tggatccata tgcggtgtga
     6061 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     6121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6721 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7021 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7081 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7141 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7201 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7261 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7321 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcagccatga gattatcaaa
     7381 aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc
     7441 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa
     7501 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc
     7561 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
     7621 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca
     7681 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     7741 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     7801 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     7861 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     7921 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     7981 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     8041 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     8101 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     8161 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     8221 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     8281 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     8341 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     8401 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     8461 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa aatttttgtt
     8521 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     8581 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     8641 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     8701 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     8761 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     8821 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     8881 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
     8941 caggatcgaa ttaattctta attaa
//
