LOCUS       Exported                3995 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses ShadowR in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    CMV-ShadowR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Murakoshi H, Horiuchi H, Kosugi T, Onda M, Sato A, Koga N, Nabekura 
            J
  TITLE     ShadowR: a novel chromoprotein with reduced non-specific binding and
            improved expression in living cells.
  JOURNAL   Sci Rep. 2019 Aug 19;9(1):12072. doi: 10.1038/s41598-019-48604-4.
  PUBMED    31427680
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        771..1074
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1075..1278
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1229..1249
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1317..1326
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(1468..1486)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1736..1755
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2031..2096
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    2221..2342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2258..2277)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2312..2331
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2349..2804
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2436..2455)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2646..2667
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2860..2964
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2965..3825
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3183..3202)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat
      661 cccctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa
      721 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      781 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      841 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      901 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      961 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     1021 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     1081 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     1141 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     1201 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     1261 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca
     1321 ccatggtgag caagggcgag gaggacaaca tggattatat taaggagttc atgcgctaca
     1381 aggtgcacat ggagggctcc gtgaacggcc acgagttcga ggtcgagggc gagggcgagg
     1441 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggcccgctgc
     1501 cgtttgcgtg ggatattctg agcccgcagt gccagtatgg cagcattccg tttaccaaat
     1561 atccggaaga tattccggat tatgtgaaac agagctttcc ggaaggcttt acctgggaac
     1621 gcgagatgaa ctttgaagat ggcgggaagg tcaccgtgag caacgatagc agcctgcagg
     1681 acggcgagtt catctacaag gtgaagctgc gcggcgagaa cttcccacct aacggccccg
     1741 taatgcagaa gaagactgag ggttgggagc ccaactccga gcggcttact ccgcatgacg
     1801 gcgccctgaa gggcaacaac cgcatgtcgc tgaaacttaa agacggcggc cattatcggg
     1861 ttgaatttaa aaccacctat aaagcgaaaa aaccagtgaa aatgccgggc catcattggg
     1921 tggatcgcaa gctggatgtg accaaccata acgaagatta taccagcgtg gaacagtatg
     1981 aacggagcga ggcgcgcaaa tccaccggcg gctcggacga gatgtacaag tccggactca
     2041 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatcctaag
     2101 cggccgcgac actagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     2161 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     2221 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     2281 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     2341 taacgcgtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2401 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     2461 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2521 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2581 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2641 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2701 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2761 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctcta gataataatg
     2821 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2881 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2941 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3001 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3061 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3121 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3181 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3241 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3301 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3361 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3421 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3481 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3541 actggcgaac tacttactct agcttcccgg caacaactga tagactggat ggaggcggat
     3601 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3661 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     3721 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3781 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     3841 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     3901 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3961 gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
