LOCUS       Exported               10463 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2%
            glucose, induced with 0.3% xylose; for homologous recombination at 
            the xyl locus in Caulobacter crescentus; tetracycline resistance.
ACCESSION   .
VERSION     .
KEYWORDS    pXGFPC-5 Pxyl-dcas9 (Sth3)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Guzzo M, Castro LK, Reisch CR, Guo MS, Laub MT.
  TITLE     A CRISPR Interference System for Efficient and Rapid Gene Knockdown 
            in Caulobacter crescentus
  JOURNAL   mBio
REFERENCE   2  (bases 1 to 10463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     oriT            21..130
                     /note="incP origin of transfer"
     primer_bind     282..298
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      299..345
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(7187..7203)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7214..7230)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(7428..8016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7508..7527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(8122..8138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttat ataagcttag
      481 cgctaccgga ctcagatcca gatcgaaatg taccggctga actggtggaa gcacgttgag
      541 cctcgtcaag gactcatgaa gcgcgtccct gctctcagtc gcctgctcgt tacgcctgag
      601 gtgtcaaaac accgtctgtt catttggcta gacgctagag ttcttccgga tcacaagctc
      661 caggtcgtta cactcgatga cgactgctca ttcggagttt tacactcaag gttccacgag
      721 gtgtgggctc ttgcagcggg gtcgtggcac ggctcaggca atgatccgcg ctacaccatc
      781 tccaccacct tcgagacctt ccccttcccg gaaggcctga cgcccaacat agccgccgtc
      841 gactatgagg gcgacccgcg cgcccaggcc atcgccgcgg cggcggccga gctgaaccgg
      901 ctgcgggaag cctggctcaa tccgcccgac ctggtgcgga tcgagcccga ggtcgtgccc
      961 ggctatcccg accgcgtgct gcccgtcagc cccgaggcgg gcgccgagct gaagaagcgc
     1021 accctgacca acctctacaa ccagcgcccg gcctggctgg acatggccca ccagcgcctg
     1081 gacgccgccg tcgccgccgc ctacggctgg cccgacggcc tgaccgacga cgagatcctg
     1141 gagcgcctgt tcgccctcaa ccaggagcgc gcggcggcgg ggcggtaggt tagacccctc
     1201 caaaaccccg acctccccgg cgaaggccgg ggtccagtta catccggaac gcttggagtg
     1261 ggcccgccta cggcctcgac caacccgcag tcggcaaggg gttcgatctg gaccccggca
     1321 ttcgccgggg aggtcggcag agaggtcaga accgcgtctt gcagccctgc ttcagatgaa
     1381 ccccgcgcag gaagccgcgg cgggccaggc cggcgtcgac ggcggcgttc atctggcggt
     1441 cggcgggggc agcgccggtg acttcgcgca ccaggccccg gaacgggatg atgctgttga
     1501 tcagggtctt gccaccggcc tcggccagct tgccggctcg gttttcggcg tactcggcgc
     1561 cgaccttgta gtcgggcccc agcgccgcgt tcagcgcctt cacctcattg gccagggcct
     1621 ggcaggactt cagacccgcg aggctgtagg ggtcgtcaaa ggccttggcc aggaccggcg
     1681 gcacctcgcg cttggacagg ccgacgtcgc gcaccggctg ggtgccgatg tcgacggcct
     1741 tcttgcccgc ctcgccccag cggccggacg gttcctcggg cttggccggc ggcttgacct
     1801 gggcgtgggc gctggaggcc agcagcacgg cggcggctcc ggccatgatc agcagcttgt
     1861 tcggcatcaa aagcgccctc ccgacaaaac acgccacggg aacggcaagc ctcgccgccg
     1921 gttcccgccc gcaagtccct cggcacgaac tctaaggcct gcgcacgatc catcgccacc
     1981 acaaagcgtg tcatcccggc cgaagcgcag cggagagccg ggacccaggg ccacaagcac
     2041 ggcgcttcgt caccccctgg gtcccggata gcgctccgcg cttccgggat gacacgggtt
     2101 taagcacttg ggtgagaagc cctagttcgg cgcgccgccc gcttccaggt cgaacgcggc
     2161 gtcgaaggcg tcgtcgccag gaaccgtccc ttccagggcg gcgtcgaaca ggtcttcgag
     2221 cgatgaccag atgaaggcca tctgctccat gatcgtcggg acgctggccg gatcgaagcc
     2281 ggggtagagg tcttccacca gccacaggcc cgtgccggga tcgaaggcga agcggcagcc
     2341 gatcaggcgg aactgggcgc gggccatggt ctcgaacagg gccgtcaggt cgcgggcggc
     2401 gtccaggtcg tcgtggtcca gcaccaggca gcggatctgc agccagccgt ggtcgggcag
     2461 caggtagaag gcgccctcgt cctgatcctc gcccgaaacc tccagccccc ggtcgatggc
     2521 ttcgacgaca tagccggccg cgcggcaggt gtcggtgagc gcggccagca gggcggcttc
     2581 ctggtcaggg gtcaggtcgg tcatgggcaa gaggtccagg tcgtggtttg tcggcggctt
     2641 ctagcatgga ccgcccgcgc ccgtgaggcc gaggatttcg cgctggtcag acaacctact
     2701 tgccgtcccc acatgttagc gctaccaagt gccgacgaac gcgcgccgcc gacggtgtcg
     2761 gcgcttcaga cgctcgagtt ttggggagac gaccatatgc ctgcaggcgc cttaattaat
     2821 atgcatggta ccttaagatc tcgagctcct gactcaggag gtatcgatat gaccaagccg
     2881 tactcgatcg gcctggccat cggcaccaac tcggtgggct gggccgtgac caccgacaac
     2941 tacaaggtgc cgtcgaagaa gatgaaggtg ctgggcaaca cctcgaagaa gtacatcaag
     3001 aagaacctgc tgggcgtgct gctgttcgac tcgggcatca ccgccgaggg ccgccgcctg
     3061 aagcgcaccg cccgccgccg ctacacccgc cgccgcaacc gcatcctgta cctgcaggag
     3121 atcttctcga ccgagatggc caccctggac gacgccttct tccagcgcct ggacgactcg
     3181 ttcctggtgc cggacgacaa gcgcgactcg aagtacccga tcttcggcaa cctggtggag
     3241 gagaaggcct accacgacga gttcccgacc atctaccacc tgcgcaagta cctggccgac
     3301 tcgaccaaga aggccgacct gcgcctggtg tacctggccc tggcccacat gatcaagtac
     3361 cgcggccact tcctgatcga gggcgagttc aactcgaaga acaacgacat ccagaagaac
     3421 ttccaggact tcctggacac ctacaacgcc atcttcgagt cggacctgtc gctggagaac
     3481 tcgaagcagc tggaggagat cgtgaaggac aagatctcga agctggagaa gaaggaccgc
     3541 atcctgaagc tgttcccggg cgagaagaac tcgggcatct tctcggagtt cctgaagctg
     3601 atcgtgggca accaggccga cttccgcaag tgcttcaacc tggacgagaa ggcctcgctg
     3661 cacttctcga aggagtcgta cgacgaggac ctggagaccc tgctgggcta catcggcgac
     3721 gactactcgg acgtgttcct gaaggccaag aagctgtacg acgccatcct gctgtcgggc
     3781 ttcctgaccg tgaccgacaa cgagaccgag gccccgctgt cgtcggccat gatcaagcgc
     3841 tacaacgagc acaaggagga cctggccctg ctgaaggagt acatccgcaa catctcgctg
     3901 aagacctaca acgaggtgtt caaggacgac accaagaacg gctacgccgg ctacatcgac
     3961 ggcaagacca accaggagga cttctacgtg tacctgaaga agctgctggc cgagttcgag
     4021 ggcgccgact acttcctgga gaagatcgac cgcgaggact tcctgcgcaa gcagcgcacc
     4081 ttcgacaacg gctcgatccc gtaccagatc cacctgcagg agatgcgcgc catcctggac
     4141 aagcaggcca agttctaccc gttcctggcc aagaacaagg agcgcatcga gaagatcctg
     4201 accttccgca tcccgtacta cgtgggcccg ctggcccgcg gcaactcgga cttcgcctgg
     4261 tcgatccgca agcgcaacga gaagatcacc ccgtggaact tcgaggacgt gatcgacaag
     4321 gagtcgtcgg ccgaggcctt catcaaccgc atgacctcgt tcgacctgta cctgccggag
     4381 gagaaggtgc tgccgaagca ctcgctgctg tacgagacct tcaacgtgta caacgagctg
     4441 accaaggtgc gcttcatcgc cgagtcgatg cgcgactacc agttcctgga ctcgaagcag
     4501 aagaaggaca tcgtgcgcct gtacttcaag gacaagcgca aggtgaccga caaggacatc
     4561 atcgagtacc tgcacgccat ctacggctac gacggcatcg agctgaaggg catcgagaag
     4621 cagttcaact cgtcgctgtc gacctaccac gacctgctga acatcatcaa cgacaaggag
     4681 ttcctggacg actcgtcgaa cgaggccatc atcgaggaga tcatccacac cctgaccatc
     4741 ttcgaggacc gcgagatgat caagcagcgc ctgtcgaagt tcgagaacat cttcgacaag
     4801 tcggtgctga agaagctgtc gcgccgccac tacaccggct ggggcaagct gtcggccaag
     4861 ctgatcaacg gcatccgcga cgagaagtcg ggcaacacca tcctggacta cctgatcgac
     4921 gacggcatct cgaaccgcaa cttcatgcag ctgatccacg acgacgccct gtcgttcaag
     4981 aagaagatcc agaaggccca gatcatcggc gacgaggaca agggcaacat caaggaggtg
     5041 gtgaagtcgc tgccgggctc gccggccatc aagaagggca tcctgcagtc gatcaagatc
     5101 gtggacgagc tggtgaaggt gatgggcggc cgcaagccgg agtcgatcgt ggtggagatg
     5161 gcccgcgaga accagtacac caaccagggc aagtcgaact cgcagcagcg cctgaagcgc
     5221 ctggagaagt cgctgaagga gctgggctcg aagatcctga aggagaacat cccggccaag
     5281 ctgtcgaaga tcgacaacaa cgccctgcag aacgaccgcc tgtacctgta ctacctgcag
     5341 aacggcaagg acatgtacac cggcgacgac ctggacatcg accgcctgtc gaactacgac
     5401 atcgacgcca tcatcccgca ggccttcctg aaggacaact cgatcgacaa caaggtgctg
     5461 gtgtcgtcgg cctcgaaccg cggcaagtcg gacgacgtgc cgtcgctgga ggtggtgaag
     5521 aagcgcaaga ccttctggta ccagctgctg aagtcgaagc tgatctcgca gcgcaagttc
     5581 gacaacctga ccaaggccga gcgcggcggc ctgtcgccgg aggacaaggc cggcttcatc
     5641 cagcgccagc tggtggagac ccgccagatc accaagcacg tggcccgcct gctggacgag
     5701 aagttcaaca acaagaagga cgagaacaac cgcgccgtgc gcaccgtgaa gatcatcacc
     5761 ctgaagtcga ccctggtgtc gcagttccgc aaggacttcg agctgtacaa ggtgcgcgag
     5821 atcaacgact tccaccacgc ccacgacgcc tacctgaacg ccgtggtggc ctcggccctg
     5881 ctgaagaagt acccgaagct ggagccggag ttcgtgtacg gcgactaccc gaagtacaac
     5941 tcgttccgcg agcgcaagtc ggccaccgag aaggtgtact tctactcgaa catcatgaac
     6001 atcttcaaga agtcgatctc gctggccgac ggccgcgtga tcgagcgccc gctgatcgag
     6061 gtgaacgagg agaccggcga gtcggtgtgg aacaaggagt cggacctggc caccgtgcgc
     6121 cgcgtgctgt cgtacccgca ggtgaacgtg gtgaagaagg tggaggagca gaaccacggc
     6181 ctggaccgcg gcaagccgaa gggcctgttc aacgccaacc tgtcgtcgaa gccgaagccg
     6241 aactcgaacg agaacctggt gggcgccaag gagtacctgg acccgaagaa gtacggcggc
     6301 tacgccggca tctcgaactc gttcaccgtg ctggtgaagg gcaccatcga gaagggcgcc
     6361 aagaagaaga tcaccaacgt gctggagttc cagggcatct cgatcctgga ccgcatcaac
     6421 taccgcaagg acaagctgaa cttcctgctg gagaagggct acaaggacat cgagctgatc
     6481 atcgagctgc cgaagtactc gctgttcgag ctgtcggacg gctcgcgccg catgctggcc
     6541 tcgatcctgt cgaccaacaa caagcgcggc gagatccaca agggcaacca gatcttcctg
     6601 tcgcagaagt tcgtgaagct gctgtaccac gccaagcgca tctcgaacac catcaacgag
     6661 aaccaccgca agtacgtgga gaaccacaag aaggagttcg aggagctgtt ctactacatc
     6721 ctggagttca acgagaacta cgtgggcgcc aagaagaacg gcaagctgct gaactcggcc
     6781 ttccagtcgt ggcagaacca ctcgatcgac gagctgtgct cgtcgttcat cggcccgacc
     6841 ggctcggagc gcaagggcct gttcgagctg acctcgcgcg gctcggccgc cgacttcgag
     6901 ttcctgggcg tgaagatccc gcgctaccgc gactacaccc cgtcgtcgct gctgaaggac
     6961 gccaccctga tccaccagtc ggtgaccggc ctgtacgaga cccgcatcga cctggccaag
     7021 ctgggcgagg gctaaatact ctggccattt ttctttaccg cctttgagtg agctgatacc
     7081 gctcgccgca gccgaacgac cgagcgcaac gcgtgcggcc gctgattagc taagcagaag
     7141 gccatcctga cggatgcctt tttcgcttgc tagctgcagc ccgggggatc cactagttct
     7201 agagcggcca ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     7261 cccaacttaa tcgccttgca gcacatcccc ctttcgccag cggtatcagc tcactcaaag
     7321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7381 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7441 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7861 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7921 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7981 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8041 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat acgaggccct
     8101 ttcgtcttca agaattaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8161 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagca gatccgtccc
     8221 tatcgtttcc acgatcagcg atcggctcgt tgccctgcgc cgctccaaag cccgcgacgc
     8281 agcgccggca ggcagagcaa gtagagggca gcgcctgcaa tccatgccca cccgttccac
     8341 gttgttatag aagccgcata gatcgccgtg aagaggaggg gtccgacgat cgaggtcagg
     8401 ctggtgagcg ccgccagtga gccttgcagc tgcccctgac gttcctcatc cacctgcctg
     8461 gacaacattg cttgcagcgc cggcattccg atgccacccg aagcaagcag gaccatgatc
     8521 gggaacgcca tccatccccg tgtcgcgaag gcaagcagga tgtagcctgt gccgtcggca
     8581 atcattccga gcatgagtgc ccgcctttcg ccgagccggg cggctacagg gccggtgatc
     8641 attgcctggg cgagtgaatg cagaatgcca aatgcggcaa gcgaaatgcc gatcgtggtc
     8701 gcgtcccagt gaaagcgatc ctcgccgaaa atgacccaaa gcgcggccgg cacctgtccg
     8761 acaagttgca tgatgaagaa gaccgccatc agggcggcga cgacggtcat gccccgggcc
     8821 caccgaacga agctgagcgg gttgagagcc tcccggcgta acggccggcg ttcgcctttg
     8881 tgcgactccg gcaaaaggaa acagcccgtc aggaaattga ggccgttcaa ggctgccgcg
     8941 gcgaagaacg gagcgtgggg ggagaaaccg cccatcagcc caccgagcac aggtcccgcg
     9001 accatcccga acccgaaaca ggcgctcatg aagccgaagt gccgcgcgcg ctcatcgcca
     9061 tcagtgatat cggcaatata agcgccggct accgccccag tcgccccggt gatgccggcc
     9121 acgatccgcc cgatatagag aacccaaagg aaaggcgctg tcgccatgat ggcgtagtcg
     9181 acagtggcgc cggccagcga gacgagcaag attggccgcc gcccgaaacg atccgacagc
     9241 gcgcccagca caggtgcgca ggcaaattgc accaacgcat acagcgccag cagaatgcca
     9301 tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg cagcaccggc
     9361 ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc tcagaattac gatcaggggt
     9421 atgttgggtt tcacgtctgg cctccggacc agcctccgct ggtccgattg aacgcgcgga
     9481 ttctttatca ctgataagtt ggtggacata ttatgtttat cagtgataaa gtgtcaagca
     9541 tgacaaagtt gcagccgaat acagtgatcc gtgccgccct agacctgttg aacgaggtcg
     9601 gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt gggggttcag cagccggcgc
     9661 tttactggca cttcaggaac aagcgggcgc tgctcgacgc actggccgaa gccatgctgg
     9721 cggagaatca tagcacttcg gtgccgagag ccgacgacga ctggcgctca tttctgactg
     9781 ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg cgatggcgcg cgcatccatg
     9841 ccggcacgcg accgggcgca ccgcagatgg aaacggccga cgcgcagctt cgcttcctct
     9901 gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct gatgacaatc agctacttca
     9961 ctgttggggc cgtgcttgag gagcaggccg gcgacagcga tgccggcgag cgcggcggca
    10021 ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc gatagacgcc ttcgacgaag
    10081 ccggtccgga cgcagcgttc gagcagggac tcgcggtgat tgtcgatgga ttggcgaaaa
    10141 ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg tgacgattga tcaggaccgc
    10201 tgccggagcg caacccactc actacagcag agccatgtag acaacatccc ctcccccttt
    10261 ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt ttcatgccct gccctagcgt
    10321 ccaagcctca cggccgcgct cggcctctct ggcggccttc tggcgctcct gctgcggcgt
    10381 ccgctcgtat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
    10441 aacgtggctt tccccccccc ccc
//
