LOCUS Exported 5051 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV asyn WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5051) AUTHORS Oueslati A, Paleologou KE, Schneider BL, Aebischer P, Lashuel HA TITLE Mimicking phosphorylation at serine 87 inhibits the aggregation of human alpha-synuclein and protects against its toxicity in a rat model of Parkinson's disease. JOURNAL J Neurosci. 2012 Feb 1;32(5):1536-44. PUBMED 22302797 REFERENCE 2 (bases 1 to 5051) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5051 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" enhancer 206..509 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 510..713 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 664..684 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 710..734 /label=LNCX /note="Human CMV promoter, forward primer" polyA_signal 1798..2274 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(1997..2016) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 2314..2454 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 2314..2443 /label=AAV2 ITR rep_origin 2529..2984 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2616..2635) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2826..2847 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3001..3020) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3120..3142 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3180..3198) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3266..3370 /gene="bla" /label=AmpR promoter CDS 3371..4231 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3589..3608) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4402..4990 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4891..4910 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtctagtt attaatagta atcaattacg gggtcattag 181 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 481 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 541 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 601 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 661 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 721 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc 781 gggaccgatc cagcctccgc ggattcgaat cccggccggg aacggtgcat tggaacgcgg 841 attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc cacaaaaaat 901 gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct 961 ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa 1021 cagtgataat ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata 1081 taaattgtaa ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca 1141 ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct 1201 tttgctaatc atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt 1261 ctgtgtgctg gcccatcact ttggcaaaga attgggattc gaacatcgat tgaattcccc 1321 ggggatcctc tagtggatct atggatgtat tcatgaaagg actttcaaag gccaaggagg 1381 gagttgtggc tgctgctgag aaaaccaaac agggtgtggc agaagcagca ggaaagacaa 1441 aagagggtgt tctctatgta ggctccaaaa ccaaggaggg agtggtgcat ggtgtggcaa 1501 cagtggctga gaagaccaaa gagcaagtga caaatgttgg aggagcagtg gtgacgggtg 1561 tgacagcagt agcccagaag acagtggagg gagcagggag cattgcagca gccactggct 1621 ttgtcaaaaa ggaccagttg ggcaagaatg aagaaggagc cccacaggaa ggaattctgg 1681 aagatatgcc tgtggatcct gacaatgagg cttatgaaat gccttctgag gaagggtatc 1741 aagactacga acctgaagcc taagaaatat ctttgctccc agtttcttga gatctacggg 1801 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg 1861 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc 1921 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc 1981 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc 2041 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg 2101 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt 2161 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg 2221 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt 2281 ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca 2341 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc 2401 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc 2461 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca 2521 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 2581 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt 2641 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 2701 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg 2761 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 2821 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt 2881 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca 2941 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag 3001 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga 3061 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 3121 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg 3181 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 3241 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 3301 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 3361 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 3421 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 3481 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 3541 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 3601 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 3661 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 3721 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 3781 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 3841 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 3901 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 3961 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 4021 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 4081 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 4141 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 4201 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 4261 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 4321 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 4381 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 4441 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 4501 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 4561 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 4621 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 4681 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 4741 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 4801 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 4861 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 4921 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 4981 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 5041 tgctcacatg t //