LOCUS Exported 10972 bp ds-DNA circular SYN 13-JAN-2022 DEFINITION Contains codon-optimized Sth1Cas9 and gRNA for streptomyces. ACCESSION . VERSION . KEYWORDS pCRISPomyces-Sth1Cas9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10972) AUTHORS Yeo WL, Heng E, Tan LL, Lim YW, Lim YH, Hoon S, Zhao H, Zhang MM, Wong FT TITLE Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes. JOURNAL Biotechnol Bioeng. 2019 May 15. doi: 10.1002/bit.27021. PUBMED 31090220 REFERENCE 2 (bases 1 to 10972) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 13, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10972 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..302 /label=rpsL promoter /note="promoter for the Xylanimonas cellulosilytica ribosomal protein S12 gene" promoter 4405..4689 /label=gapdh promoter /note="glyceraldehyde-3-phosphate dehydrogenase promoter from Eggerthella lenta" primer_bind 4880..4902 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4894..4911 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4895..4911 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 4921..4940 /label=T7 /note="T7 promoter, forward primer" promoter 4921..4939 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4987..5007) /label=T3 /note="T3 promoter, forward primer" promoter complement(4987..5005) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5026..5042) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5026..5042) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5039..5061) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5050..5066 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5074..5104) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5119..5140 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." terminator 5199..5233 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" rep_origin complement(5502..6090) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5582..5601) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS 6294..7094 /codon_start=1 /gene="aac(3)IV" /product="aminoglycoside 3-N-acetyltransferase type IV " /label=ApmR /note="confers resistance to apramycin (Paget and Davies, 1996)" /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG" CDS complement(8033..9475) /codon_start=1 /gene="rep" /product="replication initiator protein from the Streptomyces ghanaensis plasmid pSG5" /label=pSG5 Rep /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL APVLQPWGQHPGDCLRVDTAVA" oriT 10642..10751 /note="incP origin of transfer" ORIGIN 1 gccctgcagg cggaagtcag gtagacacga cttccgctag tccttgcaag gtctgctgac 61 gtgaggcggg gcggtcgttt ttgaccgccc tgccttcgtc atgtaggctc gctcgctgtg 121 cctggcgtgt catcagacgc ccaggtcccg gtgccgtgag gcccgggcca tcgagccggt 181 ggtacgtggc tgcggtcccc ttgtgagggc tgcgcgccgt gtgctgtccg gcgcgcacag 241 ccttgaatcc acccgcgggg gccggccggt ctccgtgagc tcgagtagac gacggagacg 301 taatgtccat ctaccaggag ttcgtcaaca agtactccct gtccaagacc ctgcgcttcg 361 agctgatccc gcagggcaag accctggaga acatcaaggc ccgcggcctg atcctggacg 421 acgagaagcg cgccaaggac tacaagaagg ccaagcagat catcgacaag taccaccagt 481 tcttcatcga ggagatcctg tcctccgtct gcatctccga ggacctgctc cagaactact 541 ccgacgtcta cttcaagctg aagaagtccg acgacgacaa cctccagaag gacttcaagt 601 ccgccaagga caccatcaag aagcagatca gcgagtacat caaggactcc gagaagttca 661 agaacctgtt caaccagaac ctgatcgacg ccaagaaggg ccaggagtcc gacctgatcc 721 tgtggctgaa gcagtccaag gacaacggca tcgagctgtt caaggccaac tccgacatca 781 ccgacatcga cgaggccctg gagatcatca agtccttcaa gggctggacc acctacttca 841 agggcttcca cgagaaccgc aagaacgtct actcctccaa cgacatcccg acctccatca 901 tctaccgcat cgtggacgac aacctgccga agttcctgga gaacaaggcc aagtacgagt 961 ccctgaagga caaggccccg gaggccatca actacgagca gatcaagaag gacctggccg 1021 aggagctgac cttcgacatc gactacaaga cctccgaggt caaccagcgc gtgttctccc 1081 tggacgaggt gttcgagatc gccaacttca acaactacct gaaccagtcc ggcatcacca 1141 agttcaacac catcatcggc ggcaagttcg tcaacggcga gaacaccaag cgcaagggca 1201 tcaacgagta catcaacctg tactcccagc agatcaacga caagaccctg aagaagtaca 1261 agatgtccgt cctgttcaag cagatcctgt ccgacaccga gtccaagtcc ttcgtcatcg 1321 acaagctgga ggacgactcc gacgtcgtca ccaccatgca gtccttctac gagcagatcg 1381 ccgccttcaa gaccgtcgag gagaagtcca tcaaggagac gctgtccctg ctgttcgacg 1441 acctgaaggc ccagaagctg gacctgtcca aaatctactt caagaacgac aagtccctga 1501 ccgacctgtc ccagcaggtg ttcgacgact actccgtcat cggcaccgcc gtcctggagt 1561 acatcaccca gcagatcgcc ccgaagaacc tggacaaccc gtccaagaag gagcaggagc 1621 tgatcgccaa gaaaaccgag aaggccaagt acctgtccct ggagacgatc aagctggccc 1681 tggaggagtt caacaagcac cgcgacatcg acaagcagtg ccgcttcgag gagatcctgg 1741 ccaacttcgc cgccatcccg atgatcttcg acgagatcgc ccagaacaag gacaacctgg 1801 cccagatcag catcaagtac cagaaccagg gcaagaagga cctgctccag gcctccgccg 1861 aggacgacgt caaggccatc aaggacctgc tggaccagac caacaacctg ctgcacaagc 1921 tgaaaatctt ccacatctcc cagtccgagg acaaggccaa catcctggac aaggacgagc 1981 acttctacct ggtgttcgag gagtgctact tcgagctggc caacatcgtc ccgctgtaca 2041 acaagatccg caactacatc acccagaagc cgtactccga cgagaagttc aagctgaact 2101 tcgagaactc caccctggcc aacggctggg acaagaacaa ggagccggac aacaccgcca 2161 tcctgttcat caaggacgac aagtactacc tgggcgtcat gaacaagaag aacaacaaaa 2221 tcttcgacga caaggccatc aaggagaaca agggcgaggg ctacaagaag atcgtctaca 2281 agctgctgcc gggcgccaac aagatgctgc cgaaggtgtt cttctccgcc aagtccatca 2341 agttctacaa cccgtccgag gacatcctgc gcatccgcaa ccactccacc cacaccaaga 2401 acggctcccc gcagaagggc tacgagaagt tcgagttcaa catcgaggac tgccgcaagt 2461 tcatcgactt ctacaagcag tccatctcca agcacccgga gtggaaggac ttcggcttcc 2521 gcttctccga cacccagcgc tacaactcca tcgacgagtt ctaccgcgag gtcgagaacc 2581 agggctacaa gctgaccttc gagaacatct ccgagtccta catcgactcc gtcgtcaacc 2641 agggcaagct gtacctgttc caaatctaca acaaggactt ctccgcctac tccaagggtc 2701 gcccgaacct gcacaccctg tactggaagg ccctgttcga cgagcgcaac ctccaggacg 2761 tcgtctacaa gctgaacggc gaggccgagc tgttctaccg caagcagtcc atcccgaaga 2821 agatcaccca cccggccaag gaagccatcg ccaacaagaa caaggacaac ccgaagaagg 2881 agtccgtgtt cgagtacgac ctgatcaagg acaagcgctt caccgaggac aagttcttct 2941 tccactgccc gatcaccatc aacttcaagt cctccggcgc caacaagttc aacgacgaga 3001 tcaacctgct gctgaaggag aaggccaacg acgtccacat cctgtcgatc gaccgcggcg 3061 agcgccacct ggcctactac accctggtgg acggcaaggg caacatcatc aagcaggaca 3121 ccttcaacat catcggcaac gaccgcatga aaaccaacta ccacgacaag ctggccgcca 3181 tcgagaagga ccgggactcc gcccgcaagg actggaagaa gatcaacaac atcaaggaga 3241 tgaaggaagg ctacctgtcc caggtcgtcc acgagatcgc caagctggtc atcgagtaca 3301 acgccatcgt cgtgttcgag gacctgaact tcggcttcaa gcggggccgc ttcaaggtcg 3361 agaagcaggt ctaccagaag ctggagaaga tgctgatcga gaagctgaac tacctggtgt 3421 tcaaggacaa cgagttcgac aagaccggcg gcgtcctgcg ggcctaccag ctgaccgccc 3481 cgttcgagac gttcaagaag atgggcaagc agaccggcat catctactac gtcccggccg 3541 gcttcacctc caaaatctgc ccggtcaccg gcttcgtcaa ccagctgtac ccgaagtacg 3601 agtccgtctc caagtcccag gagttcttct ccaagttcga caaaatctgc tacaacctgg 3661 acaagggcta cttcgagttc tccttcgact acaagaactt cggcgacaag gccgccaagg 3721 gcaagtggac catcgcctcc ttcggctccc gcctgatcaa cttccgcaac tccgacaaga 3781 accacaactg ggacacccgc gaggtctacc cgaccaagga gctggagaag ctgctgaagg 3841 actactccat cgagtacggc cacggcgagt gcatcaaggc cgccatctgc ggcgagtccg 3901 acaagaagtt cttcgccaag ctgacctccg tcctgaacac catcctccag atgcgcaact 3961 ccaagaccgg caccgagctg gactacctga tctccccggt cgccgacgtc aacggcaact 4021 tcttcgactc ccgccaggcc ccgaagaaca tgccgcagga cgccgacgcc aacggcgcct 4081 accacatcgg cctgaagggc ctgatgctgc tgggccgcat caagaacaac caggaaggca 4141 agaagctgaa cctggtcatc aagaacgagg agtacttcga gttcgtccag aaccgcaaca 4201 actgagaatt cagatctacg cgttccccgc aaaagcggcc tttgactccc tgcaagcctc 4261 agcgaccgaa tatatcggtt atgcgtgggc gatggttgtt gtcattgtcg gcgcaactat 4321 cggtatcaag ctgtttaaga aattcacctc gaaagcaagc tgataaaccg atacaattaa 4381 aggctccttt tggagccttt ttttgctgct ccttcggtcg gacgtgcgtc tacgggcacc 4441 ttaccgcagc cgtcggctgt gcgacacgga cggatcgggc gaactggccg atgctgggag 4501 aagcgcgctg ctgtacggcg cgcaccgggt gcggagcccc tcggcgagcg gtgtgaaact 4561 tctgtgaatg gcctgttcgg ttgctttttt tatacggctg ccagataagg cttgcagcat 4621 ctgggcggct accgctatga tcggggcgtt cctgcaattc ttagtgcgag tatctgaaag 4681 gggatacgcg tctaagaact ttaaataatt tctactgttg tagataagtc ttctcagccg 4741 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gggatcggtg 4801 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 4861 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg 4921 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cctccagctt 4981 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc 5041 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 5101 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 5161 cgctttccac cggtgaagac atactccatc tggatttgtt cagaacgctc ggttgccgcc 5221 gggcgttttt tatctagaag gtaataagaa ctacacgact ggatactgac ttttcacact 5281 agtcataact cccccagtcc tgcacgctgt cgtattctcc tggccacgac tttacaacac 5341 cgcacagcat gttgtcaaag cagagaccgt tcgaatgtga acaaagcttc atatgcatgc 5401 tagctctaga ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 5461 ctagaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 5521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 5581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 5641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 5701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 5761 gacccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 5821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 5881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 5941 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 6001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 6061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 6121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 6181 atcttcacct agatcctttt ggttcatgtg cagctccatc agcaaaaggg gatgataagt 6241 ttatcaccac cgactatttg caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat 6301 cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa agccgagctc atcggtcagc 6361 ttctcaacct tggggttacc cccggcggtg tgctgctggt ccacagctcc ttccgtagcg 6421 tccggcccct cgaagatggg ccacttggac tgatcgaggc cctgcgtgct gcgctgggtc 6481 cgggagggac gctcgtcatg ccctcgtggt caggtctgga cgacgagccg ttcgatcctg 6541 ccacgtcgcc cgttacaccg gaccttggag ttgtctctga cacattctgg cgcctgccaa 6601 atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc ggggccacag gcagagcaga 6661 tcatctctga tccattgccc ctgccacctc actcgcctgc aagcccggtc gcccgtgtcc 6721 atgaactcga tgggcaggta cttctcctcg gcgtgggaca cgatgccaac acgacgctgc 6781 atcttgccga gttgatggca aaggttccct atggggtgcc gagacactgc accattcttc 6841 aggatggcaa gttggtacgc gtcgattatc tcgagaatga ccactgctgt gagcgctttg 6901 ccttggcgga caggtggctc aaggagaaga gccttcagaa ggaaggtcca gtcggtcatg 6961 cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac agccctgggt caactgggcc 7021 gagatccgtt gatcttcctg catccgccag aggcgggatg cgaagaatgc gatgccgctc 7081 gccagtcgat tggctgagct catgagcgga gaacgagatg acgttggagg ggcaaggtcg 7141 cgctgattgc tggggcaaca cgtggagcgg atcggggatt gtctttcttc agctcgctga 7201 tgatatgctg acgctcaatg ccgtttggcc tccgactaac gaaaatcccg catttggacg 7261 gctgatccga ttggcacggc ggacggcgaa tggcggagca gacgctcgtc cgggggcaat 7321 gagatatgaa aaagcctgaa ctcaccgcga cgtatcgatg tcgaggttcc tcaggggagc 7381 caccccagag aagccctcgg agctgagcgg agctatttcc aaagccatgc cagctagaga 7441 cagtgcacac cgccaagcat ctgcaaaacc ctcgcctgga gggaaagtgc aatgtacgta 7501 cgctggtttc cctccagaaa gggattctgc ggcttctcaa ctttggaaga agaggcggaa 7561 cgaactgctg tttcagccta ctcatgtgag gaggctggtc ctttaccctg atgcccgagg 7621 gcatcagggt aaaggaccag cctcgccaac taggagcgct accaaggcga acacaaccca 7681 ggacttggta gaacccttcg ccggagaagt tcagactcat cggcatgagc acccctcgcc 7741 gcgcactcgg caccggtccg tcgaccacca ccaggacgtc gttgtcgacg tcggccccgc 7801 ggctcctgcc cgccgaacgc gtcgtcgtcg acggcctggt gctcatcgac gagcacccgg 7861 agccaggtga aaagcgccgg cggacgctcg gactgggcgc gggattccag cagtaaccca 7921 ggtccgccgg ccacctcacg gcaggcagac cctcggcttt cgcgccggga ccgccatgag 7981 accgccaccc ggatgtccgg ggtggcggtc tcatggcggt ccctcagcgg ccctacgcga 8041 ccgctgtgtc gacgcggagg cagtctccgg ggtgctggcc ccagggctgg agtaccgggg 8101 cgagcttgcc cttgcagcgg cggcacacgg gcgacgcggc gccgggcggg ggagtcttga 8161 cgtcgacggt gaccggcttc ggcttcaggc gcttccgaag gctgatcgtc gggcggatcg 8221 cctccttctt cggctgacgc actcggtcga gcgaagcggc cagctcctcc gtgcgggcct 8281 cgttggcctt ccggcgcgcc tcgcggaaag cagcttcctc ctcggacatg acctcgaacc 8341 tcatctggtc agcgtcaagg tcgcccggcg cggccggcgc ttccggggcg ggcgggtcca 8401 ggacgtcctt gccccacacc aggccccagg actcgacgag ccgccggacg cccggtaggc 8461 cgtacgtctc ggcgaccttg atgaggtcga ggcgacgtcc ggcgacgcgg gcgatgtatc 8521 ggtaccagat gtaggccggg atgaccgcga tggcgaccag gccctcggtg tcgtcggtga 8581 tctcctcctc ggtgcggacg tcctgctgga tgccgagttc cttgatcagc cggttcaggt 8641 tctgcgaccg gtagtgcttg cggacctgga acacgccgaa ctcgcgctcg cggtacttct 8701 cgacgaacgg gccgggccga cgaagccgct gcagctcggc ggccgccgcg tcgcccaggt 8761 cgagcggtcc catgcggtcg tcgccgcgac cggccttgaa gttctgtccg gccagctcca 8821 ggccgatctt ggcgacgccg cccttggtct tgtcgccgtc cttgtagagg tagcgggcct 8881 gcttgcccgc atcgccgtca gcggcgtccg cgccgttgag tgggcgcacg tcggtgccgt 8941 ggcccttgcc ctcacaggag caaccgggcc ggtcgcacgt ctcgctgacg gtgtagccgc 9001 ccgcggattc gaccccggcg gcccaggctc cggcgagtgc gtcgcggaac gcggcctggg 9061 cgtccgggcc gagcacctcg cgggtgaccc agagcgtgtg ccagtgcagg tgccagccgg 9121 agccccagcc gaaggtgtcc tcgaaggccc gctcgtagcc gatgatcccg aagtcgtcgc 9181 gcatcgtgcg ccagcggcgg ccggacgagc cgtacgcgcc cttccagccg tcgtgcaaga 9241 ccgcgaccag gccgtgccgc attcccttgc ggacggtgcc gaacgccatg cgctcgaagt 9301 ggcgcaacgt gttcgtgcca aggtgcagcc cgtacccggc gtccgcgaga ccgtcggcgg 9361 cgagctgcac gttcgagccc cgtacggcca ggatgcggct catgcaccac gggcaggtgt 9421 ggacgttgtt gcagcggcac gtgttgcccc acgtcgcctc gcccggcttc cacatcagct 9481 cggccgtccc ggcagtgagc cgggtcccgc agcccttgaa cgcctcgttc agcgacaccg 9541 tctggtgccg gtcccgccgg gcgaaccgct cgtcgcgcgg gtcctcccgc ccggctgtcg 9601 cggcaccctc gtttggggta gaacccgttc cagttacagc gctctgacct gcagtggacg 9661 gagattttcc cttactacta aagcccgcgt ccggattacc cgctgtagtc gtgcttgcta 9721 cgctgcgtga ctggtccgca atgagacgct ttgcgcgctt tcggcaggcg tccgagcagt 9781 agattttggg gcgcttcccg gggatgtgga cgatcggggt gccgcagtgg cacttcggtc 9841 cggcggggcg cggtggtgtc gacgcgctgt tctctcgtac gctcgtcaca gagcaaacgt 9901 cctcactcgg catgctgcgc cggttcgggg gcggcgagcc cgggaggcca atcccgggct 9961 cgtgccattt ctgggtcctg ttgatcctgg cattggtgtg gccgttcatt gcccctgctc 10021 gctcctgacg cgccgataga cgtccgatac gcccggtgct ggtgggattt gataggtcgg 10081 aagaagcccc gcccgggcct gggcggggct tcctgtgcgt caggacctcc tcgtcgtgag 10141 cctcttcggc ctatggacgg agtgacctcg tgatccgtta cagccgcgcg cgctcgcgta 10201 gagcggtctc atcagttcca cgaacggtcc tcttcgcaga tcagggcgtt ggggcggagt 10261 ctcaccaagg actacgtctg ctggcgattt ccgttacacc ccgggcggtg gccggcgcac 10321 acgcgcgccc gcgttgggca gtgcagaaag tgcagaaacc taggcgctga tggtccaggt 10381 ccacggttcg tcgtcggcgg cggcgcgggc ggcggcgtcg gccagggcgc gggcgagacc 10441 ggctacggcg ggcttgatgc gccggttgcg ggcgaccttg agcagctagt atgcaggtcg 10501 acggatcttt tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata 10561 tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg 10621 gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg 10681 ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg 10741 aggctggccg gctaccgccg gcgtaacaga tgagggcaag cggatggctg atgaaaccaa 10801 gccaaccagg aagggcagcc cacctatcaa ggtgtactgc cttccagacg aacgaagagc 10861 gattgaggaa aaggcggcgg cggccggcat gagcctgtcg gcctacctgc tggccgtcgg 10921 ccagggctac aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc tg //