LOCUS Exported 4677 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLCO2B1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4677) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4677) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4677 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2802..2901) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2918..2937) /label=T7 /note="T7 promoter, forward primer" promoter complement(2919..2937) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2942..2958) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2942..2958) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3060..3079) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 3071..3880 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(3138..3157) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 4027..4615 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4516..4535 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatgggcc caaggatcgg accagcagga gaggtgcctc aggtgccaga 721 caaggagacc aaggccacaa tgggaaccga gaacacacca ggaggcaagg caagcccaga 781 ccctcaggat gtgcgccctt ccgtgttcca caatatcaag ctgtttgtgc tgtgccacag 841 cctgctgcag ctggcccagc tgatgatctc tggctacctg aagagctcca tcagcaccgt 901 ggagaagagg ttcggcctgt ctagccagac atctggcctg ctggccagct ttaacgaagt 961 gggcaatacc gccctgatcg tgttcgtgtc ttactttggc agccgcgtgc accggccaag 1021 aatgatcggc tatggagcaa tcctggtggc cctggcagga ctgctgatga ccctgcctca 1081 cttcatctct gagccatacc ggtatgataa cacaagccct gaggacatgc cacaggactt 1141 caaggccagc ctgtgcctgc caaccacatc cgccccagca tctgccccta gcaacggaaa 1201 ttgttcctct tataccgaga cacagcacct gtccgtggtg ggcatcatgt tcgtggcaca 1261 gaccctgctg ggagtgggag gcgtgcccat ccagcctttc ggcatcagct acatcgacga 1321 ttttgcccac aactctaata gcccactgta tctgggcatc ctgttcgcag tgaccatgat 1381 gggaccagga ctggcctttg gcctgggatc tctgatgctg cggctgtacg tggacatcaa 1441 tcagatgcct gagggcggca tcagcctgac aatcaaggat ccaagatggg tgggagcatg 1501 gtggctgggc ttcctgatcg cagcaggagc agtggccctg gcagccatcc catatttctt 1561 tttcccaaag gagatgccca aggagaagcg ggagctgcag tttcggagaa aggtgctggc 1621 agtgaccgac tccccagcaa gaaagggcaa ggattcccca tctaagcagt cccccggcga 1681 gtctaccaag aagcaggacg gcctggtgca gatcgcccct aacctgacag tgatccagtt 1741 catcaaggtg tttccaaggg tgctgctgca gaccctgagg cacccaatct tcctgctggt 1801 ggtgctgagc caggtgtgcc tgagctccat ggcagcagga atggccatct tcctgcccaa 1861 gtttctggag agacagttca gcatcacagc ctcctacgcc aatctgctga tcggctgtct 1921 gtcctttcca tctgtgatcg tgggaatcgt ggtgggaggc gtgctggtga agaggctgca 1981 cctgggacca gtgggatgcg gcgccctgtg cctgctgggc atgctgctgt gcctgttttt 2041 ctccctgcct ctgtttttca tcggctgttc tagccaccag atcgccggca tcacccacca 2101 gacatctgcc cacccaggac tggagctgag cccttcctgc atggaggcct gcagctgtcc 2161 cctggacggc ttcaaccccg tgtgcgatcc atccaccaga gtggagtaca tcacaccttg 2221 ccacgccggc tgttcctctt gggtggtgca ggacgccctg gataactccc aggtgtttta 2281 taccaattgc tcttgcgtgg tggagggcaa tcctgtgctg gccggcagct gcgactccac 2341 atgttctcac ctggtggtgc cattcctgct gctggtgagc ctgggatccg ccctggcatg 2401 cctgacccac acaccttcct ttatgctgat cctgaggggc gtgaagaagg aggataagac 2461 cctggccgtg ggcatccagt tcatgtttct gcgcatcctg gcctggatgc catcccccgt 2521 gatccacggc tctgccatcg acaccacatg cgtgcactgg gcactgagct gcggcaggcg 2581 cgccgtgtgc cgctactata acaatgatct gctgaggaac cgcttcatcg gcctgcagtt 2641 tttctttaag accggctccg tgatctgttt tgcactggtg ctggccgtgc tgaggcagca 2701 ggacaaggag gcaaggacaa aggagagcag aagctcccca gcagtggagc agcagctgct 2761 ggtgtccgga ccaggcaaga agcctgagga ttctagagtg gacccagctt tcttgtacaa 2821 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag 2881 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat 2941 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc 3001 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac 3061 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat 3121 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac 3181 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg 3241 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat 3301 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac 3361 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa 3421 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg 3481 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg 3541 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg 3601 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt 3661 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg 3721 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt 3781 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa 3841 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt 3901 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc 3961 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 4021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 4081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 4141 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 4201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 4261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 4321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 4381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 4441 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 4501 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 4561 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 4621 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt //