LOCUS Exported 3828 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC16A13 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3828) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 3828) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3828 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1953..2052) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2069..2088) /label=T7 /note="T7 promoter, forward primer" promoter complement(2070..2088) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2093..2109) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2093..2109) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2211..2230) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2222..3031 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2289..2308) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3178..3766 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3667..3686 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggccc ggagaaccga gccacctgat ggaggatggg gatgggtggt 721 ggtgctgtcc gccttctttc agtctgccct ggtgttcggc gtgctgcggt cctttggcgt 781 gttctttgtg gagttcgtgg cagcctttga ggagcaggca gcaagagtgt cctggatcgc 841 ctctatcgga atcgcagtgc agcagttcgg aagcccagtg ggaagcgccc tgtccaccaa 901 gtttggacca aggcctgtgg tcatgacagg aggaatcctg gccgccctgg gaatgctgct 961 ggccagcttc gccacatccc tgacccacct gtacctgtcc atcggactgc tgtctggaag 1021 cggatgggca ctgacattcg cccccaccct ggcctgcctg tcttgttact tcagcaggcg 1081 ccggtccctg gcaaccggac tggccctgac aggcgtgggc ctgagctcct tcacctttgc 1141 ccctttcttt cagtggctgc tgtctcacta cgcatggagg ggaagcctgc tgctggtgtc 1201 tgccctgagc ctgcacctgg tggcctgcgg cgccctgctg cgcccaccct ctctggcaga 1261 ggacccagca gtgggaggac cacgggccca gctgacaagc ctgctgcacc acggcccttt 1321 cctgagatat acagtggccc tgaccctgat caacacaggc tactttatcc catatctgca 1381 cctggtggcc cacctgcagg acctggattg ggacccactg ccagcagcct tcctgctgag 1441 cgtggtggcc atctccgatc tggtgggcag ggtggtgtcc ggatggctgg gcgacgcagt 1501 gcctggacca gtgacccgcc tgctgatgct gtggaccaca ctgacaggcg tgtctctggc 1561 cctgtttcct gtggcacagg caccaaccgc cctggtggcc ctggcagtgg catacggctt 1621 cacatccggc gccctggcac cactggcctt ttctgtgctg cctgagctga tcggcaccag 1681 aaggatctat tgcggcctgg gcctgctgca gatgatcgag agcatcggag gactgctggg 1741 acctccactg tccggatacc tgcgggatgt gaccggcaat tatacagcca gcttcgtggt 1801 ggcaggagcc tttctgctgt ccggatctgg catcctgctg accctgccac acttcttttg 1861 tttctctacc acaaccagcg gaccacagga tctggtgacc gaggccctgg acacaaaggt 1921 gcccctgcct aaggagggcc tggaggagga cgacccagct ttcttgtaca aagttggcat 1981 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa 2041 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc 2101 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa 2161 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt 2221 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga 2281 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg 2341 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc 2401 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc 2461 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc 2521 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga 2581 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa 2641 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga 2701 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat 2761 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga 2821 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat 2881 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc 2941 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca 3001 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg 3061 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt 3121 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 3181 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 3241 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 3301 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 3361 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 3421 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 3481 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 3541 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 3601 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 3661 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 3721 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 3781 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt //