LOCUS Exported 3915 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_MFSD10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3915) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 3915) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3915 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2040..2139) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2156..2175) /label=T7 /note="T7 promoter, forward primer" promoter complement(2157..2175) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2180..2196) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2180..2196) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2298..2317) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2309..3118 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2376..2395) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3265..3853 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3754..3773 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatgggat ggggaggagg aggaggatgt accccaaggc cacctatcca 721 ccagcagcca ccagagcgga gagtggtgac cgtggtgttt ctgggcctgc tgctggacct 781 gctggccttc acactgctgc tgccactgct gcctggactg ctggagtctc acggaagggc 841 acacgaccct ctgtacggaa gctggcaggg aggcgtggat tggtttgcca cagccatcgg 901 catgccagtg gagaagcggt acaacagcgt gctgttcgga ggactgatcg gatccgcctt 961 ttctgtgctg cagttcctgt gcgcaccact gaccggagcc acaagcgatt gtctgggcag 1021 gcgccctgtg atgctgctgt gcctgatggg agtggccacc tcctacgccg tgtgggccac 1081 aagccggtcc tttgccgcct tcctggcctc tagactgatc ggcggcatca gcaagggcaa 1141 cgtgagcctg agcaccgcaa tcgtggcaga cctgggaagc ccactggcac ggtcccaggg 1201 catggccgtg atcggcgtgg ccttttccct gggcttcaca ctgggcccta tgctgggagc 1261 atctctgcct ctggagatgg caccatggtt tgccctgctg ttcgccgcct ctgatctgct 1321 gttcatcttt tgcttcctgc cagagaccct gcccctggag aagagggcac catccatcgc 1381 cctgggcttt cgcgacgcag cagatctgct gagcccactg gccctgctgc ggttctccgc 1441 cgtggcaaga ggacaggacc ctccaagcgg cgataggctg agctccctgc ggagactggg 1501 actggtgtac tttctgtatc tgtttctgtt ctccggcctg gagtacaccc tgtctttcct 1561 gacacaccag agatttcagt tctctagcct gcagcagggc aagatgttct ttctgatcgg 1621 cctgaccatg gccacaatcc agggagcata tgcaaggagg atccacccag gaggagaggt 1681 ggcagcagtg aagcgggccc tgctgctgct ggtgccagcc tttctgctga tcggctgggg 1741 cagaagcctg cccgtgctgg gactgggact gctgctgtac tccttcgccg cagcagtggt 1801 ggtgccttgt ctgtcctctg tggtggccgg ctatggctct ccaggccaga agggcaccgt 1861 gatgggcaca ctgaggagcc tgggcgccct ggcaagggca gcaggacctc tggtggcagc 1921 atccgtgtac tggctggcag gagcacaggc atgctttacc acatggtccg gcctgttcct 1981 gctgcccttc tttctgctgc agaagctgtc ttatcctgcc cagaccctga aggccgagga 2041 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa 2101 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta 2161 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa 2221 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc 2281 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc 2341 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt 2401 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt 2461 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa 2521 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga 2581 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata 2641 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc 2701 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca 2761 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg 2821 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt 2881 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg 2941 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg 3001 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat 3061 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc 3121 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc 3181 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc 3241 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 3301 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 3361 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 3421 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 3481 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 3541 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 3601 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 3661 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 3721 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 3781 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 3841 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 3901 ttttgctcac atgtt //