LOCUS Exported 3987 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_RHCG SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3987) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 3987) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3987 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2112..2211) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2228..2247) /label=T7 /note="T7 promoter, forward primer" promoter complement(2229..2247) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2252..2268) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2252..2268) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2370..2389) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2381..3190 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2448..2467) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3337..3925 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3826..3845 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggcct ggaacaccaa tctgaggtgg cgcctgccac tgacatgcct 721 gctgctgcag gtcatcatgg tcatcctgtt cggcgtgttt gtgcggtacg acttcgaggc 781 cgatgcccac tggtggtctg agagaaccca caagaacctg agcgacatgg agaatgagtt 841 ctactatcgg taccccagct ttcaggatgt ccacgtaatg gtgtttgttg gtttcggctt 901 tctgatgaca ttcctgcaga gatatggctt ttccgccgtg ggcttcaact ttctgctggc 961 cgcctttggc atccagtggg ccctgctgat gcagggctgg ttccactttc tgcaggaccg 1021 gtacatcgtg gtgggcgtgg agaacctgat caatgccgat ttctgcgtgg caagcgtgtg 1081 cgtggccttt ggcgccgtgc tgggcaaggt gtccccaatc cagctgctga tcatgacctt 1141 ctttcaggtg acactgttcg ccgtgaacga gtttatcctg ctgaatctgc tgaaggtgaa 1201 ggacgccggc ggctctatga ccatccacac attcggcgcc tactttggcc tgaccgtgac 1261 aaggatcctg tatcggagaa acctggagca gtccaaggag cgccagaata gcgtgtacca 1321 gtccgacctg ttcgccatga tcggcaccct gtttctgtgg atgtactggc ctagcttcaa 1381 ctccgccatc tcttatcacg gcgatagcca gcacagggca gccatcaata cctattgctc 1441 cctggcagca tgcgtgctga ccagcgtggc catcagctcc gccctgcaca agaagggcaa 1501 gctggacatg gtgcacatcc agaacgccac cctggcagga ggagtggcag tgggcacagc 1561 cgccgagatg atgctgatgc catacggcgc cctgatcatc ggcttcgtgt gcggcatcat 1621 ctctaccctg ggcttcgtgt atctgacacc ctttctggag agcagactgc acatccagga 1681 tacctgtggc atcaacaatc tgcacggcat ccctggaatc atcggaggaa tcgtgggagc 1741 agtgacagca gcatccgcct ctctggaggt gtacggcaag gagggcctgg tgcacagctt 1801 cgactttcag ggcttcaatg gcgattggac cgccaggaca cagggcaagt ttcagatcta 1861 tggactgctg gtgaccctgg caatggcact gatgggaggc atcatcgtgg gcctgatcct 1921 gcgcctgcct ttctggggcc agccatccga cgagaactgt tttgaggatg ccgtgtactg 1981 ggagatgcct gagggcaatt ctaccgtgta tatcccagag gatcccacat tcaagcctag 2041 cggaccatcc gtgccatctg tgcctatggt gtcccccctg cctatggcct ctagcgtgcc 2101 actggtgccc gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc 2161 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc 2221 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc 2281 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat 2341 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga 2401 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc 2461 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc 2521 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat 2581 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg 2641 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt 2701 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg 2761 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct 2821 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga 2881 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc 2941 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg 3001 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct 3061 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca 3121 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga 3181 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg 3241 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg 3301 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 3361 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 3421 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 3481 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 3541 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 3601 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 3661 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 3721 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 3781 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 3841 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 3901 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 3961 cttttgctgg ccttttgctc acatgtt //