LOCUS Exported 3885 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC52A2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3885) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 3885) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3885 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2010..2109) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2126..2145) /label=T7 /note="T7 promoter, forward primer" promoter complement(2127..2145) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2150..2166) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2150..2166) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2268..2287) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2279..3088 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2346..2365) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3235..3823 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3724..3743 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggcag cacctacccc agcaaggccc gtgctgacac acctgctggt 721 ggccctgttc ggaatgggat cctgggcagc agtgaacgga atctgggtgg agctgcctgt 781 ggtggtgaag gagctgccag agggatggag cctgccatcc tacgtgtctg tgctggtggc 841 cctgggcaat ctgggactgc tggtggtgac cctgtggcgg agactggcac ctggcaagga 901 tgagcaggtg ccaatcaggg tggtgcaggt gctgggaatg gtgggaacag ccctgctggc 961 aagcctgtgg caccacgtgg caccagtggc aggacagctg cactccgtgg ccttcctggc 1021 cctggccttt gtgctggccc tggcatgctg tgcctccaac gtgaccttcc tgccttttct 1081 gtctcacctg ccccctaggt tcctgcgctc cttctttctg ggacagggac tgtctgccct 1141 gctgccatgc gtgctggccc tggtgcaggg agtgggccgg ctggagtgtc caccagcacc 1201 catcaatgga acccctggac ctccactgga tttcctggag agatttccag cctccacctt 1261 cttttgggcc ctgacagccc tgctggtggc atctgccgca gcatttcagg gactgctgct 1321 gctgctgccc cctccaccca gcgtgcccac aggcgagctg ggctccggac tgcaagtggg 1381 agcaccagga gcagaggagg aggtggagga gagctcccca ctgcaggagc ctccatccca 1441 ggcagcagga accacaccag gacctgaccc aaaggcctac cagctgctga gcgcccggtc 1501 cgcctgcctg ctgggactgc tggccgccac caacgccctg acaaatggcg tgctgcccgc 1561 cgtgcagtcc ttctcttgtc tgccttacgg caggctggcc tatcacctgg cagtggtgct 1621 gggaagcgcc gcaaaccctc tggcatgctt cctggccatg ggcgtgctgt gccgctctct 1681 ggcaggactg ggaggactga gcctgctggg cgtgttttgc ggaggatacc tgatggcact 1741 ggccgtgctg agcccatgtc cacctctggt gggcacctcc gccggagtgg tgctggtggt 1801 gctgtcttgg gtgctgtgcc tgggcgtgtt tagctatgtg aaggtggccg cctctagcct 1861 gctgcacgga ggaggccggc ctgccctgct ggcagccggc gtggcaatcc aagtgggatc 1921 tctgctggga gcagtggcaa tgttcccacc cacatctatc tatcacgtgt ttcacagcag 1981 aaaggactgc gccgatccct gtgacagcga cccagctttc ttgtacaaag ttggcattat 2041 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat 2101 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt 2161 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 2221 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat 2281 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt 2341 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt 2401 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa 2461 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac 2521 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg 2581 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc 2641 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag 2701 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc 2761 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca 2821 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa 2881 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc 2941 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt 3001 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt 3061 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca 3121 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag 3181 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 3241 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 3301 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 3361 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 3421 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 3481 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 3541 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 3601 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 3661 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 3721 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 3781 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 3841 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt //