LOCUS Exported 3594 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC10A2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3594) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 3594) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3594 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1719..1818) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1835..1854) /label=T7 /note="T7 promoter, forward primer" promoter complement(1836..1854) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1859..1875) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1859..1875) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1977..1996) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1988..2797 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2055..2074) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2944..3532 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3433..3452 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatgaacg atcccaattc ttgcgtggac aacgcaaccg tgtgcagcgg 721 agcatcctgc gtggtgcctg agagcaattt caacaatatc ctgtccgtgg tgctgtctac 781 cgtgctgaca atcctgctgg ccctggtcat gttctccatg ggctgtaacg tggagatcaa 841 gaagtttctg ggccacatca agaggccttg gggcatctgc gtgggcttcc tgtgccagtt 901 tggcatcatg ccactgaccg gcttcatcct gtctgtggcc tttgatatcc tgccactgca 961 ggccgtggtg gtgctgatca tcggatgctg tccaggagga acagcaagca atatcctggc 1021 ctactgggtg gacggcgata tggacctgtc tgtgagcatg accacatgct ccaccctgct 1081 ggccctgggc atgatgccac tgtgcctgct gatctacaca aagatgtggg tggattccgg 1141 ctctatcgtg atcccctatg acaacatcgg cacctctctg gtgagcctgg tggtgcccgt 1201 gagcatcggc atgtttgtga atcacaagtg gcctcagaag gccaagatca tcctgaagat 1261 cggctccatc gccggcgcca tcctgatcgt gctgatcgcc gtggtgggcg gcatcctgta 1321 tcagtctgcc tggatcatcg ccccaaagct gtggatcatc ggcacaatct tccccgtggc 1381 cggctactcc ctgggctttc tgctggcaag gatcgcagga ctgccttggt atcggtgcag 1441 aaccgtggcc ttcgagacag gcatgcagaa cacccagctg tgcagcacaa tcgtgcagct 1501 gtcctttacc cctgaggagc tgaatgtggt gttcacattt ccactgatct actctatctt 1561 ccagctggcc tttgccgcca tcttcctggg cttttacgtg gcctataaga agtgccacgg 1621 caagaacaag gccgagatcc ccgagagcaa ggagaatggc accgagcctg agagctcctt 1681 ctataaggcc aacggcggct ttcagcccga cgagaaggac ccagctttct tgtacaaagt 1741 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca 1801 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt 1861 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca 1921 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag 1981 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga 2041 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat 2101 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag 2161 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc 2221 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc 2281 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat 2341 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc 2401 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt 2461 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa 2521 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc 2581 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt 2641 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc 2701 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa 2761 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta 2821 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct 2881 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 2941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 3001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 3061 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 3121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 3181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 3241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 3301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 3361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 3421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 3481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 3541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt //