LOCUS       Exported                6258 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCMV4-Tax WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Beraud C, Sun SC, Ganchi P, Ballard DW, Greene WC
  TITLE     Human T-cell leukemia virus type I Tax associates with and is 
            negatively regulated by the NF-kappa B2 p100 gene product: 
            implications for viral latency.
  JOURNAL   Mol Cell Biol. 1994 Feb . 14(2):1374-82.
  PUBMED    8289813
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             426..476
                     /codon_start=1
                     /product="nuclear localization signal from the human T-cell
                     leukemia virus type 1 (HTLV-1) Rex protein"
                     /label=Rex NLS
                     /translation="PKTRRRPRRSQRKRPPT"
     polyA_signal    1729..2351
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1928..1947)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2375..2395)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2380..2709
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2560..2695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2622..2641
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          2748..2844
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(2920..2939)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2921..2939)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2953..2969)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2953..2969)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2966..2988)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2977..2993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3001..3031)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(3184..3201)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3355..3943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3435..3454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4114..4974)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4737..4756
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4975..5079)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5106..5561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5193..5212)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5403..5424
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5687..5709
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5701..5718
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5702..5718
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5879..6258
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agaattgttt ttatttttaa
      241 ttttctttca aatacttcca tcgaattcag atctggtacc acgcgtatcg ataagcttcc
      301 tggtcttaat agccgccagt ggaaaggacc acaggaggct ctccaagaag ctgccggcgc
      361 tgctctcatc ccggtaagcg ctagttctgc ccagtggatc ccgtggagac tcctcaagcg
      421 agctgcccaa gacccgtcgg aggccccgcc gatcccaaag aaaaagacca ccaacaccat
      481 ggcccacttc ccagggtttg gacagagtct tcttttcgga tacccagtct acgtgtttgg
      541 agactgtgta caaggcgact ggtgccccat ctctggggga ctatgttcgg cccgcctaca
      601 tcgtcacgcc ctactggcca cctgtccaga gcatcagatc acctgggacc ccatcgatgg
      661 acgcgttatc ggctcagctc tacagttcct tatccctcga ctcccctcct tccccaccca
      721 gagaacctct aagaccctca aggtccttac cccgccaatc actcatacaa cccccaacat
      781 tccaccctcc ttcctccagg ccatgcgcaa atactccccc ttccgaaatg gatacatgga
      841 acccaccctt gggcagcacc tcccaaccct gtcttttcca gaccccggac tccggcccca
      901 aaacctgtac accctctggg gaggctccgt tgtctgcatg tacctctacc agctttcccc
      961 ccccatcacc tggcccctcc tgccccacgt gattttttgc caccccggcc agctcggggc
     1021 cttcctcacc aatgttccct acaagcgaat agaagaactc ctctataaaa tttcccttac
     1081 cacaggggcc ctaatgattc tccccgaaga ctgtttgccc accacccttt tccagcctgt
     1141 tagggcaccc gtcacgctaa cagcctggca aaacggcctc cttccgttcc actcaaccct
     1201 caccactcca ggccttattt ggacatttac cgatggcacg cctatgattt ccgggccctg
     1261 ccctaaagat ggccagccat ctttagtact acagtcctcc tcctttatat ttcacaaatt
     1321 tcaaaccaag gcctaccacc cctcatttct actctcacac ggccttatac agtactcttc
     1381 ctttcataat ttacatctcc tgtttgaaga atacaccaac atccccattt ctctactttt
     1441 taacgaaaaa gaggcagatg acaatgacca tgagccccaa atatcccccg ggggcttaga
     1501 gccgcccagt gaaaaacatt tccgcgaaac agaagtctga aaaggtcagg gcccagacta
     1561 aggctctgac gtctcccccc ggagggacag ctcagcaccg gctcaggcta ggccctgacg
     1621 tgtccccctg aagacaaatc ataagctcag acctccggga agccaccgga accacccatt
     1681 tcctccccat gtttgtcaag ccgccctcag gcgttggggg caggtgacgg gtggcatccc
     1741 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     1801 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     1861 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     1921 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     1981 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     2041 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     2101 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     2161 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttaaaataa
     2221 ctataccagc aggaggacgt ccagacacag cataggctac ctggccatgc ccaaccggtg
     2281 ggacatttga gttgcttgct tggcactgtc ctctcatgcg ttgggtccac tcagtagatg
     2341 cctgttgaat tgggtacgcg gccagcttgg ctgtggaatg tgtgtcagtt agggtgtgga
     2401 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2461 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2521 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     2581 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2641 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     2701 ttttgcaaaa agctcctcga ggaactgaaa aaccagaaag ttaactggta agtttagtct
     2761 ttttgtcttt tatttcaggt cccggatccg gtggtggtgc aaatcaaaga actgctcctc
     2821 agtggatgtt gcctttactt ctaggcctgt acggaagtgt tacttctgct ctaaaagctg
     2881 ctgcaggtcg actctagggc cgcgtaccga gctcgaattc cctatagtga gtcgtattaa
     2941 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3001 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaacc
     3061 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3121 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3181 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3241 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3301 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3361 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3421 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3481 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3541 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3601 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3661 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3721 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3781 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3841 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3901 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3961 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4021 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4081 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4141 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4201 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4261 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4321 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4381 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4441 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4501 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4561 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4621 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4681 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4741 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4801 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4861 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4921 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4981 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5041 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5101 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5161 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5221 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5281 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5341 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5401 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5461 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5521 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg
     5581 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     5641 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     5701 tgtaaaacga cggccagtgc caagctgatc tatacattga atcaatattg gcaattagcc
     5761 atattagtca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg
     5821 tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttga
     5881 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     5941 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     6001 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     6061 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     6121 gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     6181 cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta
     6241 gtcatcgcta ttaccatg
//
