LOCUS Exported 4041 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC17A4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4041) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4041) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4041 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2166..2265) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2282..2301) /label=T7 /note="T7 promoter, forward primer" promoter complement(2283..2301) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2306..2322) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2306..2322) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2424..2443) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2435..3244 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2502..2521) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3391..3979 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3880..3899 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatgagca ccggacctga cgtgaaggca acagtgggcg acatcagctc 721 cgatggcaac ctgaatgtgg cccaggagga gtgctccagg aagggctttt gttctgtgcg 781 ccacggactg gccctgatcc tgcagctgtg caacttctcc atctacaccc agcagatgaa 841 tctgtctatc gccatcccag ccatggtgaa caatacagca ccacctagcc agccaaacgc 901 atccaccgag aggccatcca cagactctca gggctattgg aatgagaccc tgaaggagtt 961 taaggcaatg gcaccagcat acgattggtc ccctgagatc cagggcatca tcctgtctag 1021 cctgaactat ggctcttttc tggcccctat cccaagcggc tatgtggccg gcatcttcgg 1081 agcaaagtac gtggtgggag caggactgtt catctcctct tttctgaccc tgttcatccc 1141 actggcagca aatgcaggag tggccctgct gatcgtgctg aggatcgtgc agggcatcgc 1201 ccaggtcatg gtgctgacag gccagtactc catctgggtg aagtgggcac caccactgga 1261 gcggagccag ctgaccacaa tcgccggctc tggcagcatg ctgggaagct tcatcgtgct 1321 gctggcagga ggactgctgt gccagaccat cggctggccc tacgtgttct atatctttgg 1381 aggaatcgga tgtgcatgct gtccactgtg gtttcctctg atctatgacg atccagtgaa 1441 ccaccccttc atctctgccg gcgagaagcg gtacatcgtg tgcagcctgg cccagcagga 1501 ttgttctcct ggctggagcc tgccaatcag agccatgatc aagagcctgc ctctgtgggc 1561 catcctggtg tcctactttt gcgagtattg gctgttctac acaatcatgg cctatacccc 1621 cacatacatc agctccgtgc tgcaggccaa tctgagggac tccggaatcc tgtctgccct 1681 gccttttgtg gtgggctgca tctgtatcat cctgggcggc ctgctggccg atttcctgct 1741 gagccggaag atcctgagac tgatcaccat caggaagctg tttacagcca tcggcgtgct 1801 gttcccttct gtgatcctgg tgagcctgcc atgggtgcgc tctagccaca gcatgaccat 1861 gacatttctg gtgctgtcct ctgccatcag ctccttttgt gagtccggcg ccctggtgaa 1921 cttcctggac atcgccccaa gatataccgg ctttctgaag ggcctgctgc aggtgttcgc 1981 acacatcgca ggagcaatct ccccaacagc agcaggcttc tttatcagcc aggattccga 2041 gtttggctgg aggaacgtgt tcctgctgag cgccgcagtg aatatctccg gcctggtgtt 2101 ttacctgatc ttcggcagag ccgacgtgca ggattgggcc aaggagcaga ccttcacaca 2161 cctggaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg 2221 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat 2281 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc 2341 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 2401 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 2461 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 2521 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga 2581 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 2641 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 2701 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga 2761 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 2821 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 2881 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 2941 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga 3001 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 3061 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 3121 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 3181 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 3241 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga 3301 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc 3361 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 3421 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 3481 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 3541 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 3601 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 3661 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 3721 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 3781 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 3841 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 3901 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 3961 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 4021 ctggcctttt gctcacatgt t //