LOCUS       Exported                8068 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Stable Dvl2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    pBabe-Dvl2-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Gammons MV, Rutherford TJ, Steinhart Z, Angers S, Bienz M
  TITLE     Essential role of the Dishevelled DEP domain in a Wnt-dependent 
            human-cell-based complementation assay.
  JOURNAL   J Cell Sci. 2016 Oct 15;129(20):3892-3902.
  PUBMED    27744318
REFERENCE   2  (bases 1 to 8068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     215..235
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(258..977)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(261..977)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(303..324)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     652..671
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     911..932
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      974..983
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(3275..3691)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3291..3307)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3323..3345)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3892..4091
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             4156..4625
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        4656..4760
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4761..5621
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4979..4998)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5781..6369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6270..6289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6523..6540
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             6670..7139
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(7360..7959)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7443..7463)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7940..7959
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7969..230)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7983..50
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8037..8056)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
       61 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
      121 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
      181 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacagg
      241 gtcgaccact gtgctggtta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
      301 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
      361 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      421 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
      481 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
      541 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
      601 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
      661 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
      721 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag
      781 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
      841 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
      901 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
      961 tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc
     1021 agaattcgca taacatccac aaagaactcg ctgggattgc ccatggccat gtggaagctt
     1081 tggcggctgg ctgtcagttc tgggggcaca gagcccaggt ctctgactgg aggggccccc
     1141 ggaggctgca ctgctggagg gactggaggt ggaggtgggg gcatcatgac caccatcatg
     1201 gggttgtagg ggagggccat gccagggggc ggtccatagg gatggagccc tgggtgggct
     1261 cggagattag gggcaccccc agttgagcct ctggatggag gagggccccc atcgctagtc
     1321 ccacttgctt ccccaccccg ccgaaggctg ccccctcggc tggagggctc agactcactg
     1381 ccactgccgg acttggactc gggggcccgc tcctcgggcc tccccgtgcg ccctgccccc
     1441 ccatcactcc gtgtcgaccc actgctccgg ctgccctcac tatgctggct gctggcactg
     1501 cccccaccat aggtgtaaga tgaaagctca tggtagggtg gaggctgcgg gctgtagggg
     1561 tgtggggcag ggtattggta ggagaaagtg ggcagcaggg gccagggggt ggccccaggc
     1621 agaggagcca gggtatcctg gtctgaagcc ccactggagc catcgttgtc attgagagac
     1681 aggttgacta ggtagctctc acagccacca ctgaggtctc cgaagacgta atagcactgc
     1741 tcagagaagg tgatcttgtt gacggtgtgt cggatcaggc ctgctttgag cagcccgctg
     1801 gcatacttgc gggcctcccg ccgctcagga aagccctcca cgtgatggta gagccagtca
     1861 accacatccg agcccagaaa ggcattaggg atggtgatct tgagccacat gcggtcccgg
     1921 acttccagtc cagactctgg agctgccatg gccttggtca ccgatgccat gtccgtatgg
     1981 acggagagac cccggccttc acagccatca ggcaaagacg atccagatgt aatggtgctc
     2041 atggaggagg aacctggata ggctgggaag gtgccagtca gagccgcgga atgggacacc
     2101 caggcagcag ggtcaattgg ctggatgggc tcatttcggg ggagagtgaa ataggcctga
     2161 ggagaggggt cccagcactt ggccacagtc agcacaatgg ggccaggctt gtgcacaatg
     2221 tccctcagca cccgcacagc gtcatcgttg ctcatgttct caaagttcat gtcattcacc
     2281 tgcaaaagca tgtcccctgg ctcaatgcgc ccgtcggccg ccacagcccc acccttcatg
     2341 atggagccaa tgtagatgcc tccgtctccc cgctcattgc tctggccaac aatggagata
     2401 cccaggaagt tgtacttctc catgtttagc gtgactgtga tgatattgag agacattgtg
     2461 gaatctgtga cgctgctgaa ggatgacgtc ctctccaggc ggggtggcct ctgcttcctt
     2521 cgccgccggt ggcgcttaag gaggcgggag gcactgctct gctccgtgga gctgctgaac
     2581 ctgctcatgg tgtcctcctc gtccgagtcc cccaggctgg tactctccag ctcgctggtc
     2641 atgagggtag aggagctctc gtatccggcc aggtggcgct ccagccttga ggggccacca
     2701 gtcctgtggc ccccagcgcc atgctcactg ctgtctctcc tgcgaggccg ctcccgcctc
     2761 agtgacacta ctgactcggt ttctgtctca ggctccagat tctcatggct gctggacaca
     2821 ttagggtgga aggatggagg ccttgagtcc ccaatgccgc tggtcctctc gggtggcaaa
     2881 ggaggtaaag gtggggctgg aggcgccagt tctgcccgag gctcatggac tggaggggcc
     2941 atctcgggtt ggggattatc tgaggacacc agccaggata ccacccttcc gttgaagcag
     3001 gggaggcggg cgttgtcatc tgaaatttct tccttcacca ccccgaaatc ctgatccata
     3061 gacttgaaaa agtacttggc gcccgcgggc cgctgcagga cgctcttgaa atcgccgagg
     3121 gtgatgcgct cggcggggac agggatcttc accaggtagg gagtctcttc ctcatccagg
     3181 tggtaaatca ccttcgtctc cccaacccca ccgcccccag tgctgctgcc cgcggccatc
     3241 gaattcctac gtaccaccac actgggatcc ggccggcgcc tagagaagga gtgagggctg
     3301 gataaaggga ggatcgaggc ggggtcgaac gaggaggttc aagggggaga gacggggcgg
     3361 atggaggaag aggaggcgga ggcttagggt gtacaaaggg cttgacccag ggaggggggt
     3421 caaaagccaa ggcttcccag gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc
     3481 caggtgaaaa gaccttgatc ttaacctggg tgatgaggtc tcggttaaag gtgccgtctc
     3541 gcggccatcc gacgttaaag gttggccatt ctgcagagca gaaggtaacc caacgtctct
     3601 tcttgacatc taccgactgg ttgtgagcga tccgctcgac atctttccag tgacctaagg
     3661 tcaaacttaa gggagtggta acagtctggc cctaattttc agacaaatac agaaacacag
     3721 tcagacagag acaacacaga acgatgctgc agcgctgcag cagacaagac gcgcggcttc
     3781 ggttccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg ttctcgtctc
     3841 ctaccagaac cacatatcct gacggggtcg gattccacat cgactccctt cctcaggtcg
     3901 ggccacaaaa acggccccca aagtccctgg gacgtctccc agggttgcgg ccgggtgttc
     3961 agaactcgtc agttccacca cgggtccgcc agatacagag ctagttagct aactagtacc
     4021 gacgcaggcg cataaaatca gtcatagaca ctagacaatc ggacagacac agataagttg
     4081 ctggccagct tgcctcccgg tggtgggtcg gtggtccctg ggcaggggtc tcccgatccc
     4141 ggacgagccc ccaaatgaaa gacccccgct gacgggtagt caatcactca gaggagaccc
     4201 tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta ttggatacac
     4261 gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg gttgtgggct
     4321 cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga actgattggt
     4381 tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa acatctgatg
     4441 gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc ttggccctga
     4501 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc
     4561 tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt ttggcccata
     4621 ttcaggctgc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4681 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4741 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4801 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4861 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4921 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4981 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5041 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5101 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5161 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5221 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5281 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5341 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5401 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5461 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5521 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5581 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5641 catatatact ttagattgat ttgcggccgc aaaaggatct aggtgaagat cctttttgat
     5701 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5761 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5821 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5881 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5941 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6001 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6061 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6121 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6181 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6241 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6301 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6361 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6421 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6481 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6541 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6601 tgcaactatg gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca
     6661 tgctgcatgt gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa
     6721 ggaacagcga gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac
     6781 ccgggcgact cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta
     6841 ttgagctcgg ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc
     6901 aaataaggca cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc
     6961 tagaaactgc tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg
     7021 gcaggaactg cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct
     7081 tggccctgag ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc
     7141 tgttccatct gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca
     7201 tgccttgcaa aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat
     7261 tccccccttt ttctggagac taaataaaat cttttatttt atcgatgcat ggggtcgtgc
     7321 gctcctttcg gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt
     7381 catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag
     7441 ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc
     7501 gcgctcggcc gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg
     7561 gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc
     7621 caggaggcct tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg
     7681 cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc
     7741 caccgcggcg ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag
     7801 gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg
     7861 cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc
     7921 gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg gtaagctttt tgcaaaagcc
     7981 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     8041 ggcctctgca taaataaaaa aaattagt
//
