LOCUS Exported 14824 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.. ACCESSION . VERSION . KEYWORDS pKM197 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14824) TITLE Modified / Updated Clostridium difficile CRISPR-Cas9 genome editing plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 14824) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14824 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 63..79 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 63..79 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 3574..7680 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" primer_bind complement(7690..7707) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7690..7706) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7699..7721) /label=M13/pUC Forward /note="In lacZ gene" misc_RNA 12980..13055 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" rep_origin 13253..13841 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 13742..13761 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 13995..14012 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt 61 atcaggaaac agctatgacc gcggccgcga cgtgattttt aatgggtaat ttaagtgtga 121 aatttggaag tgtagaattt aaaaatccag taataatggc gtctggaact tttggatttg 181 gaaaagaata taatgagatt tatgatatac aaaaacttgg tggtataagt agcaagggtc 241 ttactttaaa taaaaaacct ggtaacaatg gtatgagagt tcatgagaca ccatccggta 301 tgatgaatag tgttgggctt gaaaaccctg gagttcaagg atttatagac tatgaattac 361 ctttttttag taaactagac ttagtcagaa tagctaatgt tggaggtggg acattagaag 421 actacttatt aggggttcaa atgttaaatg acaagcctat agacattatt gaattaaata 481 tatcttgccc aaatgtcaaa gctggtggaa tggcttttgg tataaaaaat gaagtggcaa 541 gagaagttgt tcgtgaggta agaaacataa cgaaactgcc tttagtaata aaattatcac 601 ccaatgcaga agatattgta ggaatggcaa aagtttgtga agaagaaggt gctgatggag 661 tttcattagt taatactttt aaagcaatgg caattgatat aaaaaataga agaccagtat 721 ttgaaaatgt atatgctgga ttatctggac cagctataaa acctatagct ttgagaatgg 781 tacatgaagt gtgtaaaaat gtaaatatac ctgtaatggg aatgggcgga ataactaaag 841 ccacagatgc tatagagttt ataatggcag gagctacttg tatccaagtt ggaactgcaa 901 attttatgaa tcctagaata ggtattgaaa taatagatgg gattaatgaa tttatggata 961 gagaaggaat aaaaagttta gacgaaataa gaggaattat ataaggtaga ataaatagta 1021 ttttaaagat aaaaaatata gattaaataa acaataattt tataacatta acatggagga 1081 aaaataatta aaaacttaat tatttatagt gttacttaaa aaatgtaaat tttttagagt 1141 aaaaatttgt aatataacat ataacttaaa aataaaaagc agttagcctt ataaatgaag 1201 gttagctgtt ttttttgtta aaaatatatt tacaaaataa gagatagtag cagtacaata 1261 taagtataag gtaaaagtat tatatattag ggaggggtaa gtatgaaaaa tcaattaaat 1321 gaaaatcata aaaaggttgt attttttgga gtaggtgctg taggagctac ttttgcagaa 1381 caatttttta actctaaata tgattttaaa attctttgtg ataatgaaag aaagaaaaga 1441 tatttagaag aaggatttat aataaatgga aaaaggtatg attttaatta tgtaactaaa 1501 gatgagtata aacaagaggc tgattttata attataggtc taaaatataa taatttaaaa 1561 gaaaatataa aagaattaga tggattagtt ggaaaaaata cagttataat gtctctgcta 1621 aatggagttg atagcgaaga gataatagga gaaagatttg gaattggaaa aatggtatat 1681 tcatatgtta ccaatataga tgcaaagaaa atcaataata atattataca cactactaat 1741 gggataattg tatttggtaa caaagataat agtgaagata gaaaaactaa tataataact 1801 gaagtctttg atgatgtaaa tatagaatat attttatcaa aagatattca acgagatatg 1861 tggtggaagt acatggttaa tattggtgta aatcaaactt cagctatact tggtgcacct 1921 tatggagttt ttcagagttc tgagcattta agagaattag caaaatctgc aatgagggaa 1981 gttgttgcta tagcacaagc aaaagacata tctcttacag aagatgatgt tgaaaattca 2041 ttacatagaa tactagaaca ttcaaaagaa ggaagaacat caatgcttct cgagctagca 2101 taaaaataag aagcctgcat ttgcaggctt cttattttta tggtacgtat aatttaaagg 2161 gttatggctg tatatagtca taacccttat atttcattaa ttaaagttaa attttgtata 2221 ttcaaaaagc tcttgatatt tacggcagag ccaccatata gagatgcctc agaccctaat 2281 gatgaaattt ctatatttat tcctttgtta aaagagctaa ctaacatatc ttttgtaatc 2341 tgtaatatct cagggatatc actaattatt tggctattta aatatattat ttcaggagca 2401 taagttgtaa tagcattatt tatagctatt gttaagtaac tacaaaactc atgaataact 2461 ttttggcatt ctggttatct tcataatata gttgctttac tatatcagaa tctattttag 2521 gaatattttc taaagaagaa agctgttcaa ataccttttt ttctgaacaa tactgttcta 2581 aacagccacg atttccacaa ggacatagtt taccattagg catgatgata gtgtgaccaa 2641 tttctccact cataccattt ctgccactat ataatttatt atttattata atgcctgaac 2701 caaatccact gtgaatactg agactaagta gtgagttatg tacagttgaa aaagtattct 2761 cagctaaagc tgttaagttt gcttcatttt ctatgtgaat aggaaagtca tactttttgc 2821 taaggatgct atataagtca atctcattaa gattgtaata tggagtaaac aatactttat 2881 tttcacaagt aatcccatgt atagccaaag taagaccaat taccttataa ggagtgtcta 2941 ttttggatat gttataacta tttataattt catctatcaa ttgtataaca ttgtctttac 3001 ttacttgtat atctgttaat ttcttagaat ttataatagt tccatctaag taagatagag 3061 aagaaaatat atagtcgtat ccaatgtcca tacttaaaga aatccctgca catttattaa 3121 atactaataa tatgggtttt cttccgccac tatgagtact atttccaatt cctatttcat 3181 gaactagaga ttcatcaata agttttttgg taatagcgga tatagttgct ttattcaatc 3241 ctatagtaga agctatactt gccctagaaa taggaccatt ttttataatt tgttcaagta 3301 ccaatctttc attcatttct cgaatagtgt atttatcagt aaccaatttg atattcctcc 3361 ttaaaataat attgtaatac tttttacaca aaaataaaag gttattttgc attgacaaag 3421 ataattaaat attttattat tagttcataa gttagtttaa tatactaaca aaaataaagc 3481 aagtaaaata tacctaaaat ataaaaaaat taggatagga aaacgatagt tatgaagtgg 3541 cattcaagga gggtaaagag gagagttaat taaatggata aaaaatatag tataggatta 3601 gatataggaa caaatagtgt aggatgggca gtaataactg atgaatataa agtaccaagt 3661 aaaaaattta aagtattagg aaatacagat agacatagta taaaaaaaaa tttaataggt 3721 gcattacttt ttgatagtgg tgaaacagca gaagcaacaa gacttaaaag aacagcaaga 3781 agaagatata ctagaagaaa aaatagaata tgttatttac aagaaatatt tagtaatgaa 3841 atggcaaaag tagatgatag tttttttcat agattagaag aaagtttttt agtagaagaa 3901 gataaaaaac acgaaagaca tccaatattt ggaaatatag tagatgaagt agcatatcat 3961 gaaaaatatc caacaatata tcatttaaga aaaaaacttg tagatagtac agataaagca 4021 gatttaagac ttatatatct tgcattagca catatgataa aatttagagg acattttctt 4081 atagaaggtg atttaaatcc agataatagt gatgtagata aactttttat acaattagta 4141 caaacatata atcaattatt tgaagaaaat ccaataaatg caagtggtgt agatgcaaaa 4201 gcaatattaa gtgcaagatt aagtaaaagt agaagattag aaaatcttat agcacaatta 4261 cctggagaaa aaaaaaatgg attatttggt aatcttatag ctttaagttt aggattaaca 4321 ccaaatttta aatcaaattt tgatcttgca gaagatgcaa aacttcaatt aagtaaagat 4381 acatatgatg atgatttaga taatttactt gcacaaatag gtgatcaata tgcagattta 4441 tttcttgcag caaaaaatct ttctgatgca atacttttaa gtgatatact tagagtaaat 4501 acagaaataa caaaagcacc attatctgca agtatgataa aaagatatga tgaacatcat 4561 caagatttaa cattacttaa agcattagta agacaacaat taccagaaaa atataaagaa 4621 attttttttg atcaaagtaa aaatggatat gcaggatata tagatggtgg tgcaagtcaa 4681 gaagaatttt ataaatttat taaaccaata ttagaaaaaa tggatggaac agaagaatta 4741 ttagtaaaac ttaatagaga agatttactt agaaaacaaa gaacatttga taatggaagt 4801 ataccacatc aaatacattt aggtgaatta catgcaatat taagaagaca agaagatttt 4861 tatccatttc ttaaagataa tagagaaaaa attgaaaaaa tacttacatt tagaatacca 4921 tattatgtag gaccattagc aagaggaaat agtagatttg cttggatgac aagaaaaagt 4981 gaagaaacaa taacaccatg gaattttgaa gaagtagtag ataaaggagc atctgcacaa 5041 agttttatag aaagaatgac aaattttgat aaaaatcttc caaatgaaaa agtattacca 5101 aaacatagtt tattatatga atattttaca gtatataatg aattaacaaa agtaaaatat 5161 gtaacagaag gtatgagaaa accagcattt ttaagtggtg aacaaaaaaa agctatagta 5221 gatttattat ttaaaacaaa tagaaaagta acagtaaaac aattaaaaga agattatttt 5281 aaaaaaattg aatgttttga tagtgtagaa atatctggtg tagaagatag atttaatgca 5341 agtttaggaa catatcatga tttacttaaa ataataaagg ataaagattt tttagataat 5401 gaagaaaatg aagatatttt agaagatata gtattaacat taactttatt tgaagataga 5461 gaaatgatag aagaaagatt aaaaacttat gctcatttat ttgatgataa agtaatgaaa 5521 caacttaaaa gaagaagata tacaggttgg ggaagattaa gtagaaaact tataaatgga 5581 ataagagata aacaaagtgg aaaaacaata ttagattttc ttaaaagtga tggatttgca 5641 aatagaaatt ttatgcaatt aatacatgat gatagtttaa catttaaaga agatattcaa 5701 aaagcacaag taagtggaca aggtgatagt ttacatgaac atatagcaaa tttagcagga 5761 tctccagcaa taaaaaaagg tatattacaa acagtaaaag tagttgatga attagtaaaa 5821 gtaatgggta gacataaacc agaaaatata gttatagaaa tggcaagaga aaatcaaaca 5881 acacaaaaag gacaaaaaaa tagtagagaa agaatgaaaa gaatagaaga aggtataaaa 5941 gaattaggaa gtcaaatact taaagaacat ccagtagaaa atacacaact tcaaaatgaa 6001 aaactttatt tatattattt acaaaatgga agagatatgt atgtagatca agaattagat 6061 ataaatagat taagtgatta tgatgtagat catatagtac cacaaagttt tcttaaagat 6121 gattctatag ataataaagt attaacaaga tctgataaaa atagaggtaa aagtgataat 6181 gtaccaagtg aagaagttgt aaaaaaaatg aaaaattatt ggagacaatt acttaatgca 6241 aaacttataa cacaaagaaa atttgataat cttacaaaag cagaaagagg tggattatct 6301 gaattagata aagctggatt tataaaaaga caattagtag aaactagaca aataacaaaa 6361 catgtagcac aaatattaga tagtagaatg aatacaaaat atgatgaaaa tgataagctt 6421 ataagagaag taaaagttat aacattaaaa agtaaattag taagtgattt tagaaaagat 6481 tttcaatttt ataaagtaag agaaataaat aattatcatc atgcacatga tgcatatctt 6541 aatgcagtag taggaacagc attaataaaa aaatatccta aattagaatc tgaatttgta 6601 tatggagatt ataaagtata tgatgtaaga aaaatgatag caaaaagtga acaagaaata 6661 ggaaaagcaa cagcaaagta ctttttttat tctaatataa tgaatttttt taagacagaa 6721 ataactttag caaatggtga aataagaaaa agacctttaa tagaaactaa tggtgaaaca 6781 ggtgaaatag tatgggataa aggtagagat tttgcaacag taagaaaagt attaagtatg 6841 ccacaagtaa atatagtaaa aaaaacagaa gtacaaactg gaggatttag taaagaaagt 6901 atacttccaa aaagaaattc agataaactt attgcaagaa aaaaagattg ggatccaaaa 6961 aaatatggtg gatttgatag tccaacagta gcatatagtg tattagtagt agcaaaagta 7021 gaaaaaggta aatctaaaaa acttaaaagt gtaaaagaat tacttggaat aacaataatg 7081 gaaagaagta gttttgaaaa aaatcctatt gattttttag aagcaaaagg atataaagaa 7141 gttaaaaaag atttaataat aaaacttcca aaatattctt tatttgaatt agaaaatgga 7201 agaaaaagaa tgttagcaag tgctggtgaa ttacaaaaag gtaatgaatt agcattacca 7261 tctaaatatg taaattttct ttatttagca agtcattatg aaaaacttaa aggatctcct 7321 gaagataatg aacaaaaaca attatttgta gaacaacata aacattattt agatgaaata 7381 attgaacaaa taagtgaatt ttctaaaaga gtaatattag cagacgcaaa tttagataaa 7441 gtattatctg cttataataa acatagagat aaaccaataa gagaacaagc agaaaatata 7501 atacatcttt ttacattaac aaatttaggt gcaccagcag catttaaata ttttgataca 7561 acaatagata gaaaaagata tacaagtaca aaagaagtat tagacgcaac attaatacat 7621 caaagtataa caggattata tgaaacaaga atagatttat ctcaattagg tggtgattaa 7681 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 7741 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 7801 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct agcataaaaa 7861 taagaagcct gcatttgcag gcttcttatt tttatggcgc gcccgccctt aagtctaaaa 7921 attaggggag atgtaaggat ttgggaaaaa tagaagatgt tataatcata aatatggtat 7981 tcgtaggctt aaagtcaaaa aggaggtgaa atataaatag atttttagct aaattaagta 8041 agaaatagga ggagatttat tgaacaaaaa attagaaaaa ccatttgtat ataagagaga 8101 gtacgatttg actggatatg atgttgaaat tttacaaaaa tatgagttag aacaagcaat 8161 atatgtttat gttgggagta gttgtgcata taacatgaga gctagaagta gtaaatggag 8221 ataccatata agaacaaata ataagtctat atgttgtaac attaaaaatt ttatacataa 8281 cttggaattg ttttataaaa tggaattaaa gttgtcagat aatattatta atgataagct 8341 atactatagc aatatagcag agtttgaaga atttgaaaca ctagaaaaag ctagagaggt 8401 agaaagtact attataagtc aatatcaatt tttagattct ataaatcaca tgttaaaaca 8461 aaaaataatt ttattgagta ataaggatag tgtgttaaac ataactaaaa atggaaatac 8521 aaattatttg aaagtaaaaa ataaatacat agaaaaacat aagaacaagc caataatgag 8581 ataccatatc aactgtcaat tcaatacaga tggaagtgtc aaaagtatta cacaggagtt 8641 tgaaccaata ttggaattaa acaaaaaaaa taccctaagc cgaccaagca gagtattttt 8701 aaaataatat tttaagataa caacaaaatg agataatact actagacaat gacaactcaa 8761 ctaccaattg agtttatgga gctaccaact ccaatatcgg tctaactgat taagtatctg 8821 tagttatata ataatattgc tatcaatttt agcatcttaa caatattatt atacatacta 8881 agctaaaatt attcaatagt tgtaaaagtt gattagtcaa taagtatata tttaatgtag 8941 tgttatctct taaaaaaact agataaggag ataataaata tatggaacaa ttagattcaa 9001 aatataagtt gaaaaaattt ctaatggcag tatttagaga tggtatagga caaggaaata 9061 atcttattga taatgaatat gttagagtat ttcaaaataa taaaagtaat agtaaacaat 9121 tagaactcgg agaagaattt aaagaatata gtaaaacaac tttttttaaa aatatagatg 9181 atatagtaga atttaccttc gcaaaaaata tttattatga aaatacattt tttaacctat 9241 gtactactga tggaaaagca ggaaccaatg aaaacttaat aaatagatat gcattaggat 9301 ttgattttga caaaaaagaa ttaggacaag gttttaatta taaagatata attaatttat 9361 ttactaagat aggattacat tatcatatcc tagttgatag tggaaatgga ttccatgttt 9421 atgtgctaat taataaaact aataacatta agttagtatc agaagttaca aatacattaa 9481 taaataaatt gggtgcagat aaacaagcaa atttatctac tcaagtatta agagtacctt 9541 atacatataa tattaaaaat actactaaac aagtaaaaat aatacaccaa gacaaaaata 9601 tatatagata tgacatagaa aagttagcta aaaaatattg caaagatgta aaaacagtag 9661 gtaatactaa tacaaaatat atattagata gtaagctacc aaattgtata gtagatattt 9721 taaaaaatgg tagtaaagat ggacataaaa acctagattt gcaaaaaata gttgtgactt 9781 taagattgag gaataaaagt ttaagtcaag taatatccgt tgctagagaa tggaactata 9841 tatcacaaaa tagtctttca aatagtgagc tagaatatca agtcaagtat atgtatgaga 9901 aacttaaaac ggttaatttt ggttgtactg gttgtgagtt taatagtgat tgttggaata 9961 aaatagaatc agattttata tatagtgatg aagatacttt gttcaatatg ccacataagc 10021 actcaaagga tttgaaatat aagaatagga aaggggttaa aataatgact ggtaatcaat 10081 tgtttatcta taatgtgtta cttaacaata aagatagaga attaaacata gacgatataa 10141 tggagctgat aacctataaa cgtaagaaga aagttaaaaa cattgttatg agtgaaaaga 10201 cattaagaga aacattaaaa gaacttcaac ataatgatta tattacaaaa acaaaaggtg 10261 ttacaaagct aggaataaaa gatacataca atgtaaaaga agttagatgt aatatagata 10321 aacaatatac tattagttac tttgttacca tggcagtaat ttggggaata atttcaactg 10381 aagaattaag attatatact cacatgagat ataagcaaga tttattggtc aaagatgata 10441 aaataaaagg aaatatatta agaattaatc aagaggaatt agcaaaagat ttaggagtaa 10501 cacagcaaag aatttcaaat atgatagaat ctttattaga tactaaaatt ttagatgtat 10561 gggaaactaa aataaatgat agaggattta tgtactatac atatagatta aacaagtaga 10621 tttttgatag gattagaatt gattttctag tcctattttt atgcaaaaaa actaattaat 10681 aaaaatttct tttggtaaaa taattgtacg agaattgcaa aaaaaaaatg gcatcaaagt 10741 attgaaatta agccgtttta aaaatttctt ttggtaaaat aattctacat atatatgtag 10801 tatatatata tatgtttttt agagaatgta taactagaat atagagctag aatatagaga 10861 atgtataact agaatataga gctagaatat agagaatgta taactagaat atagagctag 10921 aatatagaga atgtataact agaatataga gctagaatat agagaatgta taactagaat 10981 atagagctag aatatagaga atgtataact agaatataga gctagaatat agagaatgta 11041 taactagaat atagagctag aatcctaatt agtaggtgct tttttaaaac aagttaaaaa 11101 tcaaaaatag tattagtaag cattggaaat gctagattct aaaatagaaa agtaaaaaat 11161 tggtgcacta tctaaactta tctatatcgc tttttccgtc gtttggttct ctagttacga 11221 tacaggggat atgcttatat tgagttatag tactaatcag tgcttaatat agttaataaa 11281 attatagtta ccatagttta gtaactatga tgtatgttag ttagaaactt gcatttcggc 11341 cggccagtgg gcaagttgaa aaattcacaa aaatgtggta taatatcttt gttcattaga 11401 gcgataaact tgaatttgag agggaactta gatggtattt gaaaaaattg ataaaaatag 11461 ttggaacaga aaagagtatt ttgaccacta ctttgcaagt gtaccttgta cctacagcat 11521 gaccgttaaa gtggatatca cacaaataaa ggaaaaggga atgaaactat atcctgcaat 11581 gctttattat attgcaatga ttgtaaaccg ccattcagag tttaggacgg caatcaatca 11641 agatggtgaa ttggggatat atgatgagat gataccaagc tatacaatat ttcacaatga 11701 tactgaaaca ttttccagcc tttggactga gtgtaagtct gactttaaat catttttagc 11761 agattatgaa agtgatacgc aacggtatgg aaacaatcat agaatggaag gaaagccaaa 11821 tgctccggaa aacattttta atgtatctat gataccgtgg tcaaccttcg atggctttaa 11881 tctgaatttg cagaaaggat atgattattt gattcctatt tttactatgg ggaaatatta 11941 taaagaagat aacaaaatta tacttccttt ggcaattcaa gttcatcacg cagtatgtga 12001 cggatttcac atttgccgtt ttgtaaacga attgcaggaa ttgataaata gttaacttca 12061 ggtttgtctg taactaaaaa caagtattta agcaaaaaca tcgtagaaat acggtgtttt 12121 ttgttaccct aagtttcata aaaataagaa gcctgcaaat gcaggcttct tatttttatg 12181 gtttaaacgg ttttagctgg gatatcggca aaacaagcaa atactactat agaagcagtt 12241 aaaagggcta ttttttataa tctggagcat agacaagaag ttagagaagc aattaaaaaa 12301 gaaaatataa gaaaaattct tattattggg acatcaaaaa aaatggttaa tcaaattgta 12361 gagagattgt caattggaag ggtctgtaag tttataaata tacaagatat aagtactaaa 12421 tatgaaatag aaatagcaaa acaagctaga aaagaaggaa atcatgtaat tcctgttcca 12481 gccgttgaga taaaaagtat ggcaagtggt ctcagtataa attctttgaa gagacttttt 12541 agaaaaggaa ataataggaa tatggtagtt ttagaaaaaa caattataag accaactttt 12601 agttatatag gaaagtttta tattagtgct gatgtaatta aacagattat tgaatatgaa 12661 gtatacaact ttggaagcat tgatagaata aataaaatca atatagagaa ttgcaataat 12721 tttatgaata tttttgtaag tataaatatc aatgatttag aaactataaa aaaaattgaa 12781 gacatacaac agacaataaa aaagtctatt gagaaaatga ctttagttaa tgtacaaaaa 12841 atagatatta acattcataa aattaaaaaa ataaacttcc tccaatgtta aaaaataaac 12901 tgagaaaatg atatactaat ttgcaataaa gtgtgctata attaaactgt aaaggtaccg 12961 aaaagtgatg cattgttggg ttttagagct agaaatagca agttaaaata aggctagtcc 13021 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttctatggag aaatctagat 13081 cagcatgatg tctgactaga cgcgtaagct ctgcaactat ttttagatgg ttgcagagct 13141 tatttttatt ttttattaaa ctcctttttg ataatctcat gaccaaaatc ccttaacgtg 13201 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 13261 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 13321 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 13381 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 13441 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 13501 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 13561 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 13621 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 13681 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 13741 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 13801 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 13861 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 13921 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 13981 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaggg 14041 ccctaacatc ttctattttt cccaaatcct tacatctccc ctaattttta gacttaaggg 14101 cgaattccaa aggacgaata tgtcgcctaa agcataatgc tctgaataag aatagaaacc 14161 atagtcctca tgaagaaaat aggacagttt cagttgtaag atgttttcga ccacctgctg 14221 tacgtctgtt gtcggaaagc gaatccttac ataatcaaac agcatttcaa ggggagcgtc 14281 gggattgaag cgttccagag cttcccaaag ggactgctgt aaatcctctg atggcttgac 14341 ttttcctgtt tcaatatcgc ttagatactg ccttgtaata ccagtcgcaa cagctaaacg 14401 gttttgagat agtccataag ccaagcgttt ttcttttaaa tgctgtaacc aagtttgttc 14461 attcagtaaa aatccctcca atcaaaaagg cgtatgtcaa cttttaaagc ccatttgaca 14521 tacgctgaaa ttttgtaaat cccttgtaac caaaggattt tctaatgttt ttttgactgt 14581 ttcctgtcga tttgtacccc cctgttagat acggggggtt aagtgctggc gtggctattg 14641 ccacaccagc cagcaagatc agtccacacc tgcgacttcc gcttcgcacg tcgcctgcgt 14701 ggactgtctg ctgttggata actttttaat ttcctccaag aaatcatatc cttttggtac 14761 aaggggagta taaaagaatt ctgcagatta cctaataatt tatctacatt ccctttaggg 14821 gccc //