LOCUS       Exported                 620 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  Gateway entry vector for nuclear targeting of domains (C-term NLS, 
            myc tags).
ACCESSION   .
VERSION     .
KEYWORDS    pENTR4-myc-nuc (w556-1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             126..146
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             150..170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             174..194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             207..236
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(288..387)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(499..518)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(577..596)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttcgttgcaa caaattgata agcaatgctt tttttataat gccaactttg tacaaaaaag
       61 caggctccac catggcccag gtgcagctgc aggtcgacct cgagatcaaa cgggcggccg
      121 cagatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa
      181 agaagagaaa ggtagatacg gccgcagaac aaaaactcat ctcagaagag gatctgaatg
      241 gggccgcata gtctagaagc tcgctgatcc ggtaccgaat tatctagacc cagctttctt
      301 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
      361 tatcagtcaa aataaaatca ttatttgcca tccagntgca gctctggccc gtgtctcaaa
      421 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
      481 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
      541 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
      601 tcgggcaatc aggtgcgaca
//
