LOCUS       Exported               11264 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gene knockout/in for actinomycetes.
ACCESSION   .
VERSION     .
KEYWORDS    pCRISPR-Cas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Tong Y, Charusanti P, Zhang L, Weber T, Lee SY
  TITLE     CRISPR-Cas9 Based Engineering of Actinomycetal Genomes.
  JOURNAL   ACS Synth Biol. 2015 Sep 18;4(9):1020-9. doi: 
            10.1021/acssynbio.5b00038. Epub 2015 Apr 7.
  PUBMED    25806970
REFERENCE   2  (bases 1 to 11264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     oriT            466..575
                     /note="incP origin of transfer"
     CDS             891..1691
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      1993..2581
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2482..2501
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2735..2752
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2869..2890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2905..2935
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2943..2959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2948..2970
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2967..2983
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2967..2983
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        3122..3197
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      3367..3401
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             3569..7672
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      7862..7910
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             9626..11068
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
ORIGIN
        1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
       61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
      121 cgaagcggta cgcgacccgt gccgggccgc agcggatcgc ggcagcagga gctccccgcc
      181 cgccacggct ggctggatgg tcgcctcacg cgcccgcagg ggtgacggcg tgggtgcggc
      241 ggtcagcccc gtgatcgggc gtcagacggc cgctgaggga ccgcgaccac ccctcccttc
      301 ccgcctgccg tctttcaccg cccggccggt ccgcagacgc cgtacggcca ggccacggag
      361 gattacgggc agatggcgcc cacccggcac ccgggctggg gcaccctgga cagcacgaag
      421 gcccgcccca ctcaccgggg cgggccttcg cgttgagcta gagttcccgc cagcctcgca
      481 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
      541 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
      601 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
      661 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaat gcagctcacg
      721 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
      781 atgccggcct ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta
      841 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
      901 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
      961 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
     1021 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctgcg ctgggtccgg
     1081 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
     1141 cgtcgcccgt tacaccggac cttggagttg tctctgacac attctggcgc ctgccaaatg
     1201 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
     1261 tctctgatcc attgcccctg ccacctcact cgcctgcaag cccggtcgcc cgtgtccatg
     1321 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
     1381 ttgccgagtt gatggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
     1441 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
     1501 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
     1561 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
     1621 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
     1681 agtcgattgg ctgagctcat aagttcctat tccgaagttc ctattcgaaa tgaccgacca
     1741 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     1801 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     1861 gctggagttc ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat
     1921 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2101 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2341 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     2401 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2461 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2521 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2581 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2641 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2701 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2761 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2821 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     2881 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     2941 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgaattgta
     3001 cgcggtcgat cttgacggct ggcgagaggt gcggggagga tctgaccgac gcggtccaca
     3061 cgtggcaccg cgatgctgtt gtgggcacaa tcgtgccggt tggtaggatc gacggccatg
     3121 ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     3181 tggcaccgag tcggtgcttt ttttacgtag gcgtcagcta cgatgttccg gggactgctg
     3241 atccggtcag caggtggaag agggactgga ttccaaagtt ctcaatgctg cttgctgttc
     3301 ttgaatgggg ggtcgttgac gacgacatgg ctcgattggc gcgacaagtt gctgcgattc
     3361 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     3421 gaggtcatta ctggaccgga tcggggatct gggctgaggg agccgacggc acgcggcggc
     3481 tcacggcgtg gcacgcggaa cgtccgggct tgcacctcac gtcacgtgag gaggcagcgt
     3541 ggacggcgtc agagaaggga gcggacatat ggacaagaag tactccatcg gcctcgacat
     3601 cggcaccaac tccgtgggct gggcggtcat caccgacgag tacaaggtcc cctccaagaa
     3661 gttcaaggtc ctgggcaaca ccgaccggca ctcgatcaag aagaacctga tcggcgccct
     3721 gctcttcgac agcggcgaga ccgccgaggc gacccgcctg aagcggaccg cgcgtcgccg
     3781 ctacacccgg cgcaagaacc gcatctgcta cctgcaggaa atcttctcca acgagatggc
     3841 caaggtggac gactcgttct tccaccgcct ggaggagagc ttcctggtgg aggaggacaa
     3901 gaagcacgag cgccacccga tcttcggcaa catcgtggac gaggtggcct accacgagaa
     3961 gtaccccacc atctaccacc tccgcaagaa gctggtggac tcgaccgaca aggcggacct
     4021 gcggctcatc tacctggccc tcgcgcacat gatcaagttc cgcggccact tcctcatcga
     4081 gggcgacctg aacccggaca actccgacgt ggacaagctc ttcatccagc tggtgcagac
     4141 ctacaaccag ctgttcgagg agaaccccat caacgccagc ggcgtggacg ccaaggcgat
     4201 cctctccgcg cgcctgagca agtcccggcg cctggagaac ctcatcgccc agctgccggg
     4261 cgagaagaag aacggcctct tcggcaacct gatcgcgctg tcgctcggcc tgacccccaa
     4321 cttcaagagc aacttcgacc tggccgagga cgcgaagctc cagctgtcca aggacaccta
     4381 cgacgacgac ctggacaacc tgctcgccca gatcggcgac cagtacgcgg acctcttcct
     4441 ggccgcgaag aacctctcgg acgccatcct gctcagcgac atcctgcggg tcaacaccga
     4501 gatcaccaag gccccgctgt cggcgagcat gatcaagcgg tacgacgagc accaccagga
     4561 cctgaccctg ctcaaggccc tcgtgcgcca gcagctgccc gagaagtaca aggaaatctt
     4621 cttcgaccag tccaagaacg gctacgccgg ctacatcgac ggcggcgcgt cgcaggagga
     4681 gttctacaag ttcatcaagc cgatcctgga gaagatggac ggcaccgagg agctgctcgt
     4741 caagctgaac cgcgaggacc tgctccgcaa gcagcggacc ttcgacaacg gctccatccc
     4801 gcaccagatc cacctgggcg agctccacgc catcctccgg cgccaggagg acttctaccc
     4861 cttcctgaag gacaaccgcg agaagatcga gaagatcctg accttccgca tcccgtacta
     4921 cgtcggcccc ctggcccgcg gcaactcccg gttcgcgtgg atgacccgga agtcggagga
     4981 gaccatcacc ccgtggaact tcgaggaggt cgtggacaag ggcgcgtccg cgcagtcgtt
     5041 catcgagcgc atgaccaact tcgacaagaa cctcccgaac gagaaggtcc tgcccaagca
     5101 ctccctgctc tacgagtact tcaccgtgta caacgagctg accaaggtca agtacgtgac
     5161 cgagggcatg cggaagccgg ccttcctgtc gggcgagcag aagaaggcga tcgtggacct
     5221 gctcttcaag accaaccgca aggtcaccgt gaagcagctg aaggaggact acttcaagaa
     5281 gatcgagtgc ttcgactccg tcgagatcag cggcgtggag gaccgcttca acgcctccct
     5341 gggcacctac cacgacctgc tcaagatcat caaggacaag gacttcctcg acaacgagga
     5401 gaacgaggac atcctggagg acatcgtcct caccctgacc ctcttcgagg accgcgagat
     5461 gatcgaggag cggctcaaga cctacgccca cctgttcgac gacaaggtga tgaagcagct
     5521 gaagcgtcgc cgctacaccg gctggggccg cctctcccgg aagctgatca acggcatccg
     5581 ggacaagcag agcggcaaga ccatcctgga cttcctcaag tccgacggct tcgccaaccg
     5641 caacttcatg cagctcatcc acgacgacag cctgaccttc aaggaggaca tccagaaggc
     5701 ccaggtctcg ggccagggcg acagcctcca cgagcacatc gccaacctgg cgggctcccc
     5761 ggcgatcaag aagggcatcc tccagaccgt caaggtcgtg gacgagctgg tcaaggtgat
     5821 gggccgccac aagcccgaga acatcgtgat cgagatggcc cgggagaacc agaccaccca
     5881 gaagggccag aagaactcgc gcgagcggat gaagcggatc gaggagggca tcaaggagct
     5941 cggcagccag atcctgaagg agcacccggt cgagaacacc cagctgcaga acgagaagct
     6001 gtacctctac tacctgcaga acggccgcga catgtacgtg gaccaggagc tcgacatcaa
     6061 ccggctgtcc gactacgacg tggaccacat cgtgccgcag tccttcctga aggacgactc
     6121 gatcgacaac aaggtcctga cccgctcgga caagaaccgg ggcaagtccg acaacgtgcc
     6181 ctcggaggag gtcgtgaaga agatgaagaa ctactggcgc cagctgctca acgccaagct
     6241 catcacccag cgcaagttcg acaacctgac caaggccgag cggggcggcc tgagcgagct
     6301 cgacaaggcg ggcttcatca agcgccagct ggtcgagacc cggcagatca ccaagcacgt
     6361 ggcccagatc ctggactccc ggatgaacac caagtacgac gagaacgaca agctgatccg
     6421 cgaggtcaag gtgatcaccc tcaagagcaa gctggtctcc gacttccgca aggacttcca
     6481 gttctacaag gtccgggaga tcaacaacta ccaccacgcc cacgacgcgt acctgaacgc
     6541 cgtcgtgggc accgcgctga tcaagaagta cccgaagctg gagtccgagt tcgtctacgg
     6601 cgactacaag gtctacgacg tgcgcaagat gatcgccaag agcgagcagg agatcggcaa
     6661 ggccaccgcg aagtacttct tctactccaa catcatgaac ttcttcaaga ccgagatcac
     6721 cctggccaac ggcgagatcc gcaagcggcc cctgatcgag accaacggcg agaccggcga
     6781 gatcgtctgg gacaagggcc gcgacttcgc caccgtccgg aaggtgctgt cgatgccgca
     6841 ggtcaacatc gtgaagaaga ccgaggtgca gaccggcggc ttcagcaagg agtccatcct
     6901 ccccaagcgc aacagcgaca agctgatcgc ccggaagaag gactgggacc cgaagaagta
     6961 cggcggcttc gacagcccca ccgtcgccta ctccgtgctg gtcgtggcga aggtcgagaa
     7021 gggcaagagc aagaagctga agtccgtgaa ggagctgctc ggcatcacca tcatggagcg
     7081 ctcctcgttc gagaagaacc cgatcgactt cctggaggcc aagggctaca aggaggtcaa
     7141 gaaggacctc atcatcaagc tgcccaagta cagcctgttc gagctggaga acggccgcaa
     7201 gcggatgctc gcctccgcgg gcgagctgca gaagggcaac gagctggccc tcccgtcgaa
     7261 gtacgtcaac ttcctgtacc tcgcgtccca ctacgagaag ctgaagggct cgcccgagga
     7321 caacgagcag aagcagctct tcgtggagca gcacaagcac tacctggacg agatcatcga
     7381 gcagatcagc gagttcagca agcgcgtcat cctggccgac gcgaacctcg acaaggtgct
     7441 gtccgcctac aacaagcacc gcgacaagcc gatccgggag caggcggaga acatcatcca
     7501 cctgttcacc ctcaccaacc tgggcgcccc cgccgcgttc aagtacttcg acaccaccat
     7561 cgaccgcaag cggtacacct ccaccaagga ggtcctcgac gcgaccctga tccaccagag
     7621 catcaccggc ctgtacgaga cccgcatcga cctgtcccag ctcggcggcg actgaaagct
     7681 ttctagagga tccccgggta ccgagctcga attccccaga tctaaagttt tgtcgtcttt
     7741 ccagacgtta gtaaatgaat tttctgtatg aggttttgct aaacaacttt caacagtttc
     7801 agcggagtga gaatagaaag gaacaactaa aggaattgcg aataataatt ttttcacgtt
     7861 gaaaatctcc aaaaaaaaag gctccaaaag gagcctttaa ttgtatcggt ttatcagctt
     7921 gctttcgagg tgaatttctt aaacagcttg ataccgatag ttgcgccgac aatgacaaca
     7981 accatcgccc acgcataacc gatatattcg gtcgctgagg cttgcaggga gtcaaaggcc
     8041 gcttttgcgg gatctcgtcg aaggcactag aaggcctata tgcggggatc gaccgcgcgg
     8101 gtcccggacg gggaagagcg gggagctttg ccagagagcg acgacttccc cttgcgttgg
     8161 tgattgccgg tcagggcagc catccgccat cgtcgcgtag ggtgtcacac cccaggaatc
     8221 gcgtcactga acacagcagc cggtaggacg accatgactg agttggacac catcgcaaat
     8281 ccgtccgatc ccgcggtgca gcggatcatc gatgtcacca agccgtcgcg atccaacata
     8341 aagacaacgt tgatcgagga cgtcgagccc ctcatgcaca gcatcgcggc cggggtggag
     8401 ttcatcgagg tctacggcag cgacagcagt ccttttccat ctgagttgct ggatctgtgc
     8461 gggcggcaga acataccggt ccgcctcatc gactcctcga tcgtcaacca gttgttcaag
     8521 ggggagcgga aggccaagac attcggcatc gcccgcgtcc ctcgcccggc caggttcggc
     8581 gatatcgcga gccggcgtgg ggacgtcgtc gttctcgacg gggtgaagat cgtcgggaac
     8641 atcggcgcga tagtacgcac gtcgctcgcg ctcggagcgt cggggatcat cctggtcgac
     8701 agtgacatca ccagcatcgc ggaccggcgt ctccaaaggg ccagccgagg ttacgtcttc
     8761 tcccttcccg tcgttctctc cggtcgcgag gaggccatcg ccttcattcg ggacagcggt
     8821 atgcagctga tgacgctcaa ggcggatggc gacatttccg tgaaggaact cggggacaat
     8881 ccggatcggc tggccttgct gttcggcagc gaaaagggtg ggccttccga cctgttcgag
     8941 gaggcgtctt ccgcctcggt ttccatcccc atgatgagcc agaccgagtc tctcaacgtt
     9001 tccgtttccc tcggaatcgc gctgcacgag aggatcgaca ggaatctcgc ggccaaccga
     9061 taagcgcctc tgttcctcgg acgctcggtt gctcgacctc gattcgtcag tgatgatcaa
     9121 caggacccag aaatggcacg agcccgggat tggcctcccg ggctcgccgc ccccgaaccg
     9181 gcgcagcatg ccgagtgagg acgtttgctc tgtgacgagc gtacgagaga acagcgcgtc
     9241 gacaccaccg cgccccgccg gaccgaagtg ccactgcggc accccgatcg tccacatccc
     9301 cgggaagcgc cccaaaatct actgctcgga cgcctgccga aagcgcgcaa agcgtctcat
     9361 tgcggaccag tcacgcagcg tagcaagcac gactacagcg ggtaatccgg acgcgggctt
     9421 tagtagtaag ggaaaatctc cgtccactgc aggtcagagc gctgtaactg gaacgggttc
     9481 taccccaaac gagggtgccg cgacagccgg gcgggaggac ccgcgcgacg agcggttcgc
     9541 ccggcgggac cggcaccaga cggtgtcgct gaacgaggcg ttcaagggct gcgggacccg
     9601 gctcactgcc gggacggccg agctgatgtg gaagccgggc gaggcgacgt ggggcaacac
     9661 gtgccgctgc aacaacgtcc acacctgccc gtggtgcatg agccgcatcc tggccgtacg
     9721 gggctcgaac gtgcagctcg ccgccgacgg tctcgcggac gccgggtacg ggctgcacct
     9781 tggcacgaac acgttgcgcc acttcgagcg catggcgttc ggcaccgtcc gcaagggaat
     9841 gcggcacggc ctggtcgcgg tcttgcacga cggctggaag ggcgcgtacg gctcgtccgg
     9901 ccgccgctgg cgcacgatgc gcgacgactt cgggatcatc ggctacgagc gggccttcga
     9961 ggacaccttc ggctggggct ccggctggca cctgcactgg cacacgctct gggtcacccg
    10021 cgaggtgctc ggcccggacg cccaggccgc gttccgcgac gcactcgccg gagcctgggc
    10081 cgccggggtc gaatccgcgg gcggctacac cgtcagcgag acgtgcgacc ggcccggttg
    10141 ctcctgtgag ggcaagggcc acggcaccga cgtgcgccca ctcaacggcg cggacgccgc
    10201 tgacggcgat gcgggcaagc aggcccgcta cctctacaag gacggcgaca agaccaaggg
    10261 cggcgtcgcc aagatcggcc tggagctggc cggacagaac ttcaaggccg gtcgcggcga
    10321 cgaccgcatg ggaccgctcg acctgggcga cgcggcggcc gccgagctgc agcggcttcg
    10381 tcggcccggc ccgttcgtcg agaagtaccg cgagcgcgag ttcggcgtct tccaggtccg
    10441 caagcactac cggtcgcaga acctgaaccg gctgatcaag gaactcggca tccagcagga
    10501 cgtccgcacc gaggaggaga tcaccgacga caccgagggc ctggtcgcca tcgcggtcat
    10561 cccggcctac atctggtacc gatacatcgc ccgcgtcgcc ggacgtcgcc tcgacctcat
    10621 caaggtcgcc gagacgtacg gcctaccggg cgtccggcgg ctcgtcgagt cctggggcct
    10681 ggtgtggggc aaggacgtcc tggacccgcc cgccccggaa gcgccggccg cgccgggcga
    10741 ccttgacgct gaccagatga ggttcgaggt catgtccgag gaggaagctg ctttccgcga
    10801 ggcgcgccgg aaggccaacg aggcccgcac ggaggagctg gccgcttcgc tcgaccgagt
    10861 gcgtcagccg aagaaggagg cgatccgccc gacgatcagc cttcggaagc gcctgaagcc
    10921 gaagccggtc accgtcgacg tcaagactcc cccgcccggc gccgcgtcgc ccgtgtgccg
    10981 ccgctgcaag ggcaagctcg ccccggtact ccagccctgg ggccagcacc ccggagactg
    11041 cctccgcgtc gacacagcgg tcgcgtaggg ccgctgaggg accgccatga gaccgccacc
    11101 ccggacatcc gggtggcggt ctcatggcgg tcccggcgcg aaagccgagg gtctgcctgc
    11161 cgtgaggtgg ccggcggacc tgggttactg ctggaatccc gcgcccagtc cgagcgtccg
    11221 ccggcgcttt tcacctggct ccgggtgctc gtcgatgagc acca
//
