LOCUS Exported 8602 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Fluorescent reporter of Agrobacterium rhizogenes mediated transformed roots - modified. ACCESSION . VERSION . KEYWORDS pHairyRed (double 35s removed) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8602) TITLE Gresshoff/Ferguson plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 8602) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8602 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(39..56) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(39..55) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(48..70) /label=M13/pUC Forward /note="In lacZ gene" promoter 432..777 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 720..740 /label=35S promoter /note="CaMV 35S promoter, forward primer" intron 809..998 /label=cat1 intron /note="castor bean catalase intron, modified" CDS 1054..1731 /codon_start=1 /product="improved tetrameric variant of DsRed fluorescent protein" /label=DsRed2 /note="mammalian codon-optimized" /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH LFL" primer_bind complement(1234..1254) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 1646..1669 /label=DsRed1-C /note="DsRed1, forward primer" terminator 1767..2019 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 2041..2065 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 3366..3995 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 4424..5497 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 5563..5757 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(5993..6015) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 6101..6241 /label=bom /note="basis of mobility region from pBR322" primer_bind 6156..6175 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(6256..6273) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6427..7015) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6507..6526) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7102..7896) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 8321..8345 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" polyA_signal 8423..8597 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" ORIGIN 1 gatcctctag agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt 61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 181 ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag 241 tttagcttca tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact 301 ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac 361 atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac 421 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat 481 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa 541 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc 601 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 661 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 721 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg 781 gggactcttg accatggtag atctgagggt aaatttctag tttttctcct tcattttctt 841 ggttaggacc cttttctctt tttatttttt tgagctttga tctttcttta aactgatcta 901 ttttttaatt gattggttat ggtgtaaata ttacatagct ttaactgata atctgattac 961 tttatttcgt gtgtctatga tgatgatgat agttacagaa ccgacgaact agtaacggcc 1021 gccagtgtgc tggaattcgc ccttcatgca actatggcct cctccgagaa cgtcatcacc 1081 gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga acggccacga gttcgagatc 1141 gagggcgagg gcgagggccg cccctacgag ggccacaaca ccgtgaagct gaaggtgacc 1201 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc 1261 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag 1321 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac cgtgacccag 1381 gactcctccc tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc 1441 ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc caccgagcgc 1501 ctgtaccccc gcgacggcgt gctgaagggc gagacccaca aggccctgaa gctgaaggac 1561 ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg 1621 cccggctact actacgtgga cgccaagctg gacatcacct cccacaacga ggactacacc 1681 atcgtggagc agtacgagcg caccgagggc cgccaccacc tgttcctgta gccacgtgtg 1741 aattggtgac cagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa 1801 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta 1861 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta 1921 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg 1981 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattaaac tatcagtgtt 2041 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat 2101 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca 2161 gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg 2221 cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac 2281 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat 2341 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc 2401 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc 2461 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg 2521 cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt 2581 gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat 2641 ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc 2701 ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat 2761 catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc 2821 ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg 2881 ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc 2941 acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga 3001 ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca 3061 agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag 3121 gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg 3181 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg 3241 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc 3301 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg 3361 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg 3421 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg 3481 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg 3541 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg 3601 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg 3661 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg 3721 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg 3781 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt 3841 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg 3901 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg 3961 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag 4021 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac 4081 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga 4141 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct 4201 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa 4261 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg 4321 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc 4381 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc 4441 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc 4501 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca 4561 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc 4621 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga 4681 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg 4741 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc 4801 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc 4861 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt 4921 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca 4981 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg 5041 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag 5101 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc 5161 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc 5221 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc 5281 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc 5341 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc 5401 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg 5461 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca 5521 aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat 5581 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta 5641 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc 5701 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact 5761 gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc 5821 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg 5881 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg 5941 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 6001 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 6061 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 6121 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 6181 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 6241 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 6301 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 6361 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 6421 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 6481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 6541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 6601 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 6661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 6721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 6781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 6841 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 6901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 6961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 7021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 7081 tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat attttatttt 7141 ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc 7201 gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc 7261 gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc 7321 tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc 7381 atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc 7441 ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt 7501 atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc 7561 gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc 7621 ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac 7681 aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt 7741 ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt 7801 atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag 7861 ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta 7921 cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc 7981 cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg 8041 gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg 8101 ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg 8161 gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta 8221 caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt 8281 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg 8341 taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta atgtactgaa 8401 ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt tttaggaatt 8461 agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg tttcttatat 8521 gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg aactactcac 8581 acattattat ggagaaactc ga //