LOCUS Exported 9266 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Neutrophil specific label. ACCESSION . VERSION . KEYWORDS p5E LyzC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9266) AUTHORS Oehlers SH, Cronan MR, Scott NR, Thomas MI, Okuda KS, Walton EM, Beerman RW, Crosier PS, Tobin DM TITLE Interception of host angiogenic signalling limits mycobacterial growth. JOURNAL Nature. 2015 Jan 29;517(7536):612-5. doi: 10.1038/nature13967. Epub 2014 Nov 24. PUBMED 25470057 REFERENCE 2 (bases 1 to 9266) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9266 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 6472..6488 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 6473..6489 /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6523..6539) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6523..6539) /label=pBluescriptSK /note="For pBluescript vector" promoter complement(6576..6594) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" protein_bind 6594..6718 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter complement(6791..6809) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6814..6830) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6814..6830) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6932..6951) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 6943..7752 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(7010..7029) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 7899..8487 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8388..8407 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8641..8658 /label=L4440 /note="L4440 vector, forward primer" terminator 8817..8844 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 8936..9022 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 9071..9093 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 9085..9102 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 9086..9102 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 9142..9237 /gene="mutant version of attL" /label=attL4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 9239..9257 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 cctgatcact ggtgtagtga actcatcaag ggttaatgag cagttgcaga ctgaactgtg 61 aaggtggagt tggtttatct ctgtttgcaa agttatttca ttttacttgg ttataaacag 121 caggcatgtg cagaaggggg gtttgggtgc ttgagtacct gctctttttt tctctcgaag 181 agaaagtacc ccataaaata aaaagtgccc cctcgtttgt gcacggatcc cctatacgca 241 acaccatcca acgctcttta gcgtcgcgat taacccgtgc cctaaccgtt aaccaaattg 301 gttaacaagc accagttttg ccttcaattt ttttttctaa atgagcaact aactttctgt 361 ggtaaagtaa ttgccctgtg tgccatactg ctgctaagga gtagatgatg tctgcagcgc 421 agaatgatga cgcatgtcac tcaaagatta tttaaaagtc acatgaaatc cgacataacc 481 gttcacactg cggtcacatt gcaaaacatt agatcttcac attaatgaaa aatttgacat 541 cctggttcta tattgtattt gcaagaagtt attcaattca agatttccaa atgtacaaaa 601 atgaaatgtg aaaatgtgtg aatatgctgt ctttcactaa taatggcatc ctttaaaaat 661 aagagcaagg aaaatatcta tatttattgt aaaccttttc atcttctgca tttgatgaat 721 tgttgaacat ctgagcagtg tgaaatcttc taacaaatgg cctgatgtaa tctgaccata 781 tttttaactt aaaacatctt tttagccttg agtttactat gttgggcagt ttgatcatct 841 tttaccacaa ctttcttatc tgaatccatt gccaacgttt tgttgccgat catctgtgca 901 gttcgaattg accctaacgt gaccaccaac aaggattgaa ggctcaagtg tacccgtttt 961 tcttgtaata atcatcttgc ccacattgta aaaaatgctg ggttccacac aattccttca 1021 tgttgtccca acacaagtcg attaaattaa tttattaaat ggattgaaca taaaacaatt 1081 cagttatgcc ccccaaaaaa tcaagagctg tgttgattta gctcatttta aactattagg 1141 tttaacaagc agcaaaaaaa aaatatatat atatatatat atttcaatac agacatcaga 1201 aacacatctc aatatagcag ttcttcaaat ttctgccctt ggtttgacca cagactgtat 1261 ttgactcttt tctttatttt atagtcactt cacatgttct aaactgataa taaaacacaa 1321 taatattcag ctgttcacta agttttgaca catcatcttt ctttcattcc taaaaatgac 1381 ctgtaaacac tacaatgaac acgtctttgc taaaaccgta tatgcagagc aaatgaatta 1441 aaaaaaaatg taaaggtcct gtgaagtgct ttgaaatgtg cattttttat ttgatgtttg 1501 atgtaatctt aaccaaaaca cagagagagg gtggaacatt gcatagctcc tcccctttaa 1561 aaaaacagcc agtagtgttt tgttttacca cagtgagagt ggttgagctc aagcgcatca 1621 aattaaaagc aaatgagaag catcttgaag ggggcggggc ttgtcagaca ctagagagaa 1681 tttgattggt tatgatgtaa tgagaaactg aagtgtgaca tgaataaaac cattgatcca 1741 tttaggtgga actgacaaac tacaagcttt acatgtttat gtgtttatca tcatctaaac 1801 gcaaagttta tctctgtttt ggagcacaca agcttataga tatcttaaaa acgaataata 1861 ctgatactaa catctaaaaa cgttatttta ttttcatggg acctttaaat attctgccgt 1921 tttatcttca cacaagaccg ttttcatgcc aactttgctt tgtctcagtt cttctgcttt 1981 ggaaacaaaa tttaacgctg ctgcgtgtaa aagtttcaat tcttttcact cgtcacagta 2041 caaagacacg gaagtgtcgt gatggaagaa aaaaactggg tgggaaaaaa atggaaaaca 2101 ctcagtgata ggaatttttt tttttttttg gtttttgcgt ttttctcgca aagatatttg 2161 catttccttg caaaattgtt gttccacaaa cacttcctgt tcacttcata catgtcaacc 2221 cttccgtttt tgccggtgtg gtattttacc actctatccc gctatcatcc tatcaataaa 2281 cattttccca tatttctctc atattttaaa tcttgaaagc acattgaaat taatatataa 2341 atgtttgcat tcgctttatt tccattaaag ctgtttgtcg atcatcagcc tttatatgca 2401 acctctgaat gcatgtataa gcaagggaac gcaaaaactt taaaatattt atttttctat 2461 cacttttttt tcttccaccc atttttcctt caccatcaca tcccttaggt ctccgtaaca 2521 cagcatcatg ggctaaattt ttttcttctg tgattcatat cgttctcagt tgagtgtaaa 2581 taatcctgtt gtaataactg atatttgcag gttcatatca acacaaacca ctgagccgat 2641 ttattgtttc tcatattttg ctataaaccc ttcatctact ctatgaaaaa aacaacacaa 2701 gaaatgattc atcgtttttc tggaatcaat cataagtaca gatctaaatg gtttattgct 2761 tcatgagaca atatagtttt ctccgtggca acctcagtgt agtagcacaa caaacaagag 2821 gttgatcatg gggaaaatgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 2881 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 2941 gtgtgtgtgt gtgttgtgtt agctttggtc agaggaggct gctgtgtttg gccagagctc 3001 tgctggccag ggtcagaatc ctgcttgttg aagagcctct tcatcatgtg gatccagaaa 3061 ctgaagattt gctgcaacag gtgatccacg cggagtttaa agactgcaca gtcctgtttc 3121 tcagtcaaaa cccactcagt gtcctgcaca cggacaggtc agaaaccaca ctgagacacg 3181 tcctgttatt aatagtcaag tttgagggat ttatgatgtg gtgttgtttt cttcagggtg 3241 ctggttttgg acaagggaca agctgtgaag tttgatgctc cttccactct cctccagcag 3301 gggccgctgt tcagccaata agatcagacc tgctcttaca ctacagttaa tattgacaaa 3361 tatttcagcg tatccatttc acatcatatt ttaggaacag taaaacacag tatttattaa 3421 tcagttatga gtattttaag ttaatttgaa tattaatatc aatcagtttt tagttaatga 3481 aggttaaatt acaacaaaca gattttgaaa tattctagca agttgtgagg attactttca 3541 atttttacat ttcagctgat tttcgttttg tttatatata tatatatata tatatatata 3601 tatatatata tatatatata tatatatata ttaaaatata attatctaca ttttgatttg 3661 tagtgattaa tttttattga tattaatttt atattattat tagaagtagt aaattaagtt 3721 ttaattataa caaacaaatt ttaagtattg taagttgtga gggttacttt gactttgttt 3781 acattccttt cagctacttt acattttgtt atttatttat ttgtattcct tttatattta 3841 tttgctaata taattatgta gatttttata atgtgttatt ttaatttttt aaattttttt 3901 aatgttaaat gtatattgta tatttagatt tattattaag ttgattagta tgatgtatga 3961 atttattact gaaggttttt taacaattaa ttgaagcaaa acaatattta ataagcacag 4021 acataccagt ttacagagta actccagtaa aggactggtt tatttatttc atttttcgcc 4081 atacatttga ttagaagagt ttgacttggg ttgattatag caaatagtgg aacacaaaca 4141 gattttgaaa tattcttgca aatttattta ttaaatccat tcaaatattg tcattttttg 4201 tactgtgtaa tttatattga tttgctcata caatatttac atttcgctta aacaatacac 4261 tattatttat atttaaaaat agctatatat aaatcaaatg tatagctctt ttttacacaa 4321 ttaatcaaag ctaaaatatt tgtcacacaa atacagtaag ccacttcaca atgatatctt 4381 caaaatctgt gcttgactga atcatttcat ttattgtttt gtaattcaca ttatacctta 4441 cgttctccag gcacagtttg aattgttgat aaatgaagat aatacatctg atttaattag 4501 tctcctttgt ttataataac tttaatctaa tttgatagta ataattccag caatacatcc 4561 attacaataa aaatcgcttt tttattttgc acattagttt gccggtattt gtttaaagtt 4621 tcaccatact tttaaatcat gtgctatttg aaaagggctg ttatgactgg aattaaatgt 4681 ttttttttat atcagtgttt tagattgaag tacaatattt ataggtaaaa ttcaccagca 4741 tatgcttaaa gatagccatg ctccaggtcc ttgtgtcctt ctaaggtctg tttgtggttt 4801 aagctgtttg ttgacacacg cttgtcttct ccatttttct gtgaagttat tgtcagttta 4861 ttgcacactg ggttgcatta agctgcagtt ttttcacaac agttttccac attgtaacag 4921 tcagtctcca aattattcca tcagtcctca aatttagtca tttgaggcca tgaaaacact 4981 taaaactaca tgaaggtagt ttggcattag gttttagact tgttcttgtt tggcatattg 5041 caacctgtgt aaaaatgatt tgatttttat tttttttttt tttttttcag aaagaaacgg 5101 tccaaaaaga gttatgtgaa catttatcca tgtgtttaat gttttttaaa ttaaaaaaaa 5161 acatttttat taaaccactt gtgactaaac cttttttagt tattctattt tgtgacgagt 5221 ctgaatggcc aaagtactct tgaatccaaa atctttatag taaaatgttt aatcttactt 5281 gcagtgttgg gtataatttg taacaaagtt gaattccata tctgctgagt gaaaaagtaa 5341 agtaagagat tgctgtaaat atttaggtta tttatttatt aatacaatat agaaagtaac 5401 aaaacacaat gtaaaaaatc aataaagaaa gtaaatatta aaagtaatga taaactaatg 5461 atgtaaacaa tattagcaac aaagtttgta caattcaggg agggtaaaga atttcaccta 5521 atagtcaaac tctgtattaa ataatataaa atctttgttc ttagtctgaa gaggagactg 5581 tgagagaaag cttactggtt gttaaattag aaaatttgta acttgcagat taaaatgatt 5641 taaataattt tataaaggcc atgtttgaaa caaatgtaaa aacataataa atgttaagaa 5701 tttcagatat tgagatttta caatttaaaa aaagcaataa agtaatagca gagacctaca 5761 ctcttaaagt aaattaccca actctgctta ctagtaactt ttggaatagt tactagtatc 5821 cattgaacaa atactagaat ctaattaaat tgtgtgagtg tccaatgaaa agattcttgt 5881 atttaaaaca gtaccagtat ctatatatgt acaaaattta gtgctagcaa aatgcaaata 5941 aatactactg catataatgg attgcattgt aaatcgactc gccataatac atttatttcc 6001 tatttttact tttaacaaaa aataagttca ctgagtattt catgtcatta aatcgcatta 6061 aacctcttgc atgctttatg tacactagtg cagttcgcta aatgaccacc ggtgtcacta 6121 ataacacgat cgtttctgaa ttctgcatta aacgccattt atacgttgct cttattagta 6181 aacaatttct ccatcatgag ctcgaccagt attgcatatt tcatcctctt aaatccagag 6241 ttgtttcttt taatcacttt ctcttcatga taaaagcagt ttttggtttt cagaagttta 6301 caacaaaccc agtcagttat tgcctcagca aacactggag caactgcaca cttgtgaaaa 6361 gcttcttgcg aaacacaaac ctttctctgg acttataaat aaaggcattc agcagctcca 6421 ccatacacct ccactggtct tgataaagca gatatcagca gtgatacaat ctcgaggtcg 6481 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag 6541 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattcaagtt 6601 tgtacaaaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga 6661 ttttgcataa aaaacagact acataatact gtaaaacaca acatatgcag tcactatgaa 6721 ccaactactt agatggtatt agtgacctgt agaattcgag ctctagagct gcagggcggc 6781 cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg 6841 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca 6901 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg 6961 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg 7021 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat 7081 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag 7141 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc 7201 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag 7261 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg 7321 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt 7381 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac 7441 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc 7501 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag 7561 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat 7621 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt 7681 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat 7741 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact 7801 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac 7861 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 7921 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 7981 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 8041 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 8101 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 8161 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 8221 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 8281 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 8341 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 8401 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 8461 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 8521 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 8581 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 8641 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 8701 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 8761 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa 8821 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc 8881 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg 8941 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg 9001 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca 9061 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggcccgcg 9121 ttaacgctac catggagctc caaataatga ttttattttg actgatagtg acctgttcgt 9181 tgcaacaaat tgataagcaa tgctttttta taatgccaac tttgtataga aaagttggta 9241 atacgactca ctatagggcg aattgg //