LOCUS Exported 3273 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Cyan fluorescent tag. ACCESSION . VERSION . KEYWORDS pME turquoise2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3273) AUTHORS Oehlers SH, Cronan MR, Scott NR, Thomas MI, Okuda KS, Walton EM, Beerman RW, Crosier PS, Tobin DM TITLE Interception of host angiogenic signalling limits mycobacterial growth. JOURNAL Nature. 2015 Jan 29;517(7536):612-5. doi: 10.1038/nature13967. Epub 2014 Nov 24. PUBMED 25470057 REFERENCE 2 (bases 1 to 3273) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3273 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 674..1393 /codon_start=1 /product="enhanced monomeric variant of CFP (Goedhart et al., 2012)" /label=mTurquoise2 /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(719..740) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(980..999) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1327..1348 /label=EGFP-C /note="EGFP, forward primer" protein_bind complement(1398..1497) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1514..1533) /label=T7 /note="T7 promoter, forward primer" promoter complement(1515..1533) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1538..1554) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1538..1554) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1656..1675) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1667..2476 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1734..1753) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2623..3211 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3112..3131 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctgg accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 721 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 781 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 841 gccctggccc accctcgtga ccaccctgtc ctggggcgtg cagtgcttcg cccgctaccc 901 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 961 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 1021 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 1081 catcctgggg cacaagctgg agtacaacta ctttagcgac aacgtctata tcaccgccga 1141 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg 1201 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 1261 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg 1321 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 1381 gctctacaag tagatctacc cagctttctt gtacaaagtt ggcattataa gaaagcattg 1441 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca 1501 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct 1561 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg 1621 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca 1681 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 1741 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc 1801 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 1861 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 1921 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt 1981 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 2041 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt 2101 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga 2161 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc 2221 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 2281 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 2341 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 2401 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct 2461 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct 2521 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt 2581 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 2641 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 2701 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 2761 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 2821 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 2881 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 2941 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 3001 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 3061 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 3121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 3181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 3241 cctggccttt tgctggcctt ttgctcacat gtt //