LOCUS Exported 5134 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Nucleus/Histones, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-H3-23 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5134) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 5134) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5134 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 37..340 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 341..544 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 495..515 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 574..583 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 991..1043 /label=MCS /note="multiple cloning site" regulatory 1051..1060 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1057..1776 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1102..1123) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1363..1382) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1710..1731 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 1898..2019 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1935..1954) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1989..2008 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2026..2481) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2163..2184) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2375..2394 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2508..2612 /gene="bla" /label=AmpR promoter promoter 2614..2971 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2637..2657) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2822..2957 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2884..2903 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3006..3800 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3060..3079) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3670..3689 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3988..4007) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4032..4079 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4408..4996 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4897..4916 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 61 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 121 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 181 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 241 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 301 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta 361 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga 421 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa 481 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag 541 agctggttta gtgaaccgtc agatccgcta gccgccacca tggctcgtac taaacagaca 601 gctcggaaat ccaccggcgg taaagcgcca cgcaagcagc tggctaccaa ggctgctcgc 661 aagagcgcgc cggctaccgg cggcgtgaaa aagcctcacc gttaccgccc gggcactgtg 721 gctctgcgcg agatccgccg ctaccaaaag tcgaccgagt tgctgattcg gaagctgccg 781 ttccagcgcc tggtgcgaga aatcgcccaa gacttcaaga ccgatcttcg cttccagagc 841 tctgcggtga tggcgctgca ggaggcttgt gaggcctact tggtagggct ctttgaggac 901 acaaaccttt gcgccatcca tgctaagcga gtgactatta tgcccaaaga catccagctc 961 gctcgccgca ttcgcggaga aagagcggat ctcgagctca agcttcgaat tctgcagtcg 1021 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg 1081 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 1141 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1201 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc 1261 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1321 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1381 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1441 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 1501 cacaaggtct atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc 1561 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1621 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg 1681 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1741 gggatcactc tcggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat 1801 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct 1861 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa 1921 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 1981 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta 2041 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 2101 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 2161 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 2221 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 2281 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 2341 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 2401 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 2461 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 2521 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 2581 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa 2641 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 2701 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 2761 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 2821 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 2881 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 2941 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt 3001 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 3061 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 3121 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 3181 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 3241 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 3301 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 3361 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 3421 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 3481 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc 3541 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 3601 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 3661 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 3721 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 3781 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc 3841 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 3901 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 3961 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac 4021 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca 4081 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 4141 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg 4201 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata 4261 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 4321 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 4381 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 4441 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 4501 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 4561 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 4621 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 4681 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 4741 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 4801 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 4861 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 4921 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 4981 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 5041 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 5101 cgccatgcat tagttattaa tagtaatcaa ttac //