LOCUS       Exported               13933 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway destination clone of CDK12 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    p667-UBC-CDK12-V5-miniTurbo_IDG-K
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13933)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 13933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13933
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4018..4134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4166..4186
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4186..5397
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5234..5253
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5398..5422
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9917..9941)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9943..9984
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             9991..10761
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(10767..10787)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    10816..11404
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10869..10889)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11287..11298)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        11417..11916
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11452..11475)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11849..11868
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11937..12536
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11937..11956)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12433..12453
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             12674..12907
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12979..13100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13016..13035)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13070..13089
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13119..13254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13181..13200
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(13286..13305)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13287..13305)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13315..13332)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13315..13331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13324..13346)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13476..13931
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13563..13582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13773..13794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
     4141 cagtgtggtg gaattctgca gatatcaact ttgtatagaa aagttggcct ccgcgccggg
     4201 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
     4261 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
     4321 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
     4381 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
     4441 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
     4501 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
     4561 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
     4621 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
     4681 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
     4741 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
     4801 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
     4861 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
     4921 actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt
     4981 gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt
     5041 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc
     5101 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac
     5161 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat
     5221 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt
     5281 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag
     5341 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgca
     5401 actttgtaca aaaaagcagg cttagccacc atgcccaatt cagagagaca tgggggcaag
     5461 aaggacggga gtggaggagc ttctggaact ttgcagccgt catcgggagg cggcagctct
     5521 aacagcagag agcgtcaccg cttggtatcg aagcacaagc ggcataagtc caaacactcc
     5581 aaagacatgg ggttggtgac ccccgaagca gcatccctgg gcacagttat caaacctttg
     5641 gtggagtatg atgatatcag ctctgattcc gacaccttct ccgatgacat ggccttcaaa
     5701 ctagaccgaa gggagaacga cgaacgtcgt ggatcagatc ggagcgaccg cctgcacaaa
     5761 catcgtcacc accagcacag gcgttcccgg gacttactaa aagctaaaca gaccgaaaaa
     5821 gaaaaaagcc aagaagtctc cagcaagtcg ggatcgatga aggaccggat atcgggaagt
     5881 tcaaagcgtt cgaatgagga gactgatgac tatgggaagg cgcaggtagc caaaagcagc
     5941 agcaaggaat ccaggtcatc caagctccac aaggagaaga ccaggaaaga acgggagctg
     6001 aagtctgggc acaaagaccg gagtaaaagt catcgaaaaa gggaaacacc caaaagttac
     6061 aaaacagtgg acagcccaaa acggagatcc aggagccccc acaggaagtg gtctgacagc
     6121 tccaaacaag atgatagccc ctcgggagct tcttatggcc aagattatga ccttagtccc
     6181 tcacgatctc atacctcgag caattatgac tcctacaaga aaagtcctgg aagtacctcg
     6241 agaaggcagt cggtcagtcc cccttacaag gagccttcgg cctaccagtc cagcacccgg
     6301 tcaccgagcc cctacagtag gcgacagaga tctgtcagtc cctatagcag gagacggtcg
     6361 tccagctacg aaagaagtgg ctcttacagc gggcgatcgc ccagtcccta tggtcgaagg
     6421 cggtccagca gccctttcct gagcaagcgg tctctgagtc ggagtccact ccccagtagg
     6481 aaatccatga agtccagaag tagaagtcct gcatattcaa gacattcatc ttctcatagt
     6541 aaaaagaaga gatccagttc acgcagtcgt cattccagta tctcacctgt caggcttcca
     6601 cttaattcca gtctgggagc tgaactcagt aggaaaaaga aggaaagagc agctgctgct
     6661 gctgcagcaa agatggatgg aaaggagtcc aagggttcac ctgtattttt gcctagaaaa
     6721 gagaacagtt cagtagaggc taaggattca ggtttggagt ctaaaaagtt acccagaagt
     6781 gtaaaattgg aaaaatctgc cccagatact gaactggtga atgtaacaca tctaaacaca
     6841 gaggtaaaaa attcttcaga tacagggaaa gtaaagttgg atgagaactc cgagaagcat
     6901 cttgttaaag atttgaaagc acagggaaca agagactcta aacccatagc actgaaagag
     6961 gagattgtta ctccaaagga gacagaaaca tcagaaaagg agacccctcc acctcttccc
     7021 acaattgctt ctcccccacc ccctctacca actactaccc ctccacctca gacaccccct
     7081 ttgccacctt tgcctccaat accagctctt ccacagcaac cacctctgcc tccttctcag
     7141 ccagcattta gtcaggttcc tgcttccagt acttcaactt tgcccccttc tactcactca
     7201 aagacatctg ctgtgtcctc tcaggcaaat tctcagcccc ctgtacaggt ttctgtgaag
     7261 actcaagtat ctgtaacagc tgctattcca cacctgaaaa cttcaacgtt gcctcctttg
     7321 cccctcccac ccttattacc tggagatgat gacatggata gtccaaaaga aactcttcct
     7381 tcaaaacctg tgaagaaaga gaaggaacag aggacacgtc acttactcac agaccttcct
     7441 ctccctccag agctccctgg tggagatctg tctcccccag actctccaga accaaaggca
     7501 atcacaccac ctcagcaacc atataaaaag agaccaaaaa tttgttgtcc tcgttatgga
     7561 gaaagaagac aaacagaaag cgactggggg aaacgctgtg tggacaagtt tgacattatt
     7621 gggattattg gagaaggaac ctatggccaa gtatataaag ccaaggacaa agacacagga
     7681 gaactagtgg ctctgaagaa ggtgagacta gacaatgaga aagagggctt cccaatcaca
     7741 gccattcgtg aaatcaaaat ccttcgtcag ttaatccacc gaagtgttgt taacatgaag
     7801 gaaattgtca cagataaaca agatgcactg gatttcaaga aggacaaagg tgccttttac
     7861 cttgtatttg agtatatgga ccatgactta atgggactgc tagaatctgg tttggtgcac
     7921 ttttctgagg accatatcaa gtcgttcatg aaacagctaa tggaaggatt ggaatactgt
     7981 cacaaaaaga atttcctgca tcgggatatt aagtgttcta acattttgct gaataacagt
     8041 gggcaaatca aactagcaga ttttggactt gctcggctct ataactctga agagagtcgc
     8101 ccttacacaa acaaagtcat tactttgtgg taccgacctc cagaactact gctaggagag
     8161 gaacgttaca caccagccat agatgtttgg agctgtggat gtattcttgg ggaactattc
     8221 acaaagaagc ctatttttca agccaatctg gaactggctc agctagaact gatcagccga
     8281 ctttgtggta gcccttgtcc agctgtgtgg cctgatgtta tcaaactgcc ctacttcaac
     8341 accatgaaac cgaagaagca atatcgaagg cgtctacgag aagaattctc tttcattcct
     8401 tctgcagcac ttgatttatt ggaccacatg ctgacactag atcctagtaa gcggtgcaca
     8461 gctgaacaga ccctacagag cgacttcctt aaagatgtcg aactcagcaa aatggctcct
     8521 ccagacctcc cccactggca ggattgccat gagttgtgga gtaagaaacg gcgacgtcag
     8581 cgacaaagtg gtgttgtagt cgaagagcca cctccatcca aaacttctcg aaaagaaact
     8641 acctcaggga caagtactga gcctgtgaag aacagcagcc cagcaccacc tcagcctgct
     8701 cctggcaagg tggagtctgg ggctggggat gcaataggcc ttgctgacat cacacaacag
     8761 ctgaatcaaa gtgaattggc agtgttatta aacctgctgc agagccaaac cgacctgagc
     8821 atccctcaaa tggcacagct gcttaacatc cactccaacc cagagatgca gcagcagctg
     8881 gaagccctga accaatccat cagtgccctg acggaagcta cttcccagca gcaggactca
     8941 gagaccatgg ccccagagga gtctttgaag gaagcaccct ctgccccagt gatcctgcct
     9001 tcagcagaac agacgaccct tgaagcttca agcacaccag ctgacatgca gaatatattg
     9061 gcagttctct tgagtcagct gatgaaaacc caagagccag caggcagtct ggaggaaaac
     9121 aacagtgaca agaacagtgg gccacagggg ccccgaagaa ctcccacaat gccacaggag
     9181 gaggcagcag catgtcctcc tcacattctt ccaccagaga agaggccccc tgagcccccc
     9241 ggacctccac cgccgccacc tccaccccct ctggttgaag gcgatctttc cagcgccccc
     9301 caggagttga acccagccgt gacagccgcc ttgctgcaac ttttatccca gcctgaagca
     9361 gagcctcctg gccacctgcc acatgagcac caggccttga gaccaatgga gtactccacc
     9421 cgaccccgtc caaacaggac ttatggaaac actgatgggc ctgaaacagg gttcagtgcc
     9481 attgacactg atgaacgaaa ctctggtcca gccttgacag aatccttggt ccagaccctg
     9541 gtgaagaaca ggaccttctc aggctctctg agccaccttg gggagtccag cagttaccag
     9601 ggcacagggt cagtgcagtt tccaggggac caggacctcc gttttgccag ggtcccctta
     9661 gcgttacacc cggtggtcgg gcaaccattc ctgaaggctg agggaagcag caattctgtg
     9721 gtacatgcag agaccaaatt gcaaaactat ggggagctgg ggccaggaac cactggggcc
     9781 agcagctcag gagcaggcct tcactggggg ggcccaactc agtcttctgc ttatggaaaa
     9841 ctctatcggg ggcctacaag agtcccacca agagggggaa gagggagagg agttccttac
     9901 tcaggctcga gcggcaaccc agctttcttg tacaaagtgg gcggcaagcc catccccaac
     9961 cccctgctgg gcctggacag caccgctagc atcccgctgc tgaacgctaa acagattctg
    10021 ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg tcgactccac caatcagtac
    10081 ctgctggatc gaatcggcga gctgaagagt ggggatgctt gcattgcaga atatcagcag
    10141 gcagggagag gaagcagagg gaggaaatgg ttctctcctt ttggagctaa cctgtacctg
    10201 agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc ggtcatcgga
    10261 attgtcatgg cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt caaatggccc
    10321 aatgacctgt atctgcagga tagaaagctg gcaggcatcc tggtggagct ggccggaata
    10381 acaggcgatg ctgcacagat cgtcattggc gccgggatta acgtggctat gaggcgcgtg
    10441 gaggaaagcg tggtcaatca gggctggatc acactgcagg aagcagggat taacctggac
    10501 aggaatactc tggccgctat gctgatccga gagctgcggg cagccctgga actgttcgag
    10561 caggaaggcc tggctccata tctgtcacgg tgggagaagc tggataactt catcaataga
    10621 cccgtgaagc tgatcattgg ggacaaagag attttcggga ttagccgggg gattgataaa
    10681 cagggagccc tgctgctgga acaggacgga gttatcaaac cctggatggg cggagaaatc
    10741 agtctgcggt ctgccgaaaa gtaagccaac tttattatac atagttgata tccagcacag
    10801 tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
    10861 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
    10921 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
    10981 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
    11041 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
    11101 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
    11161 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
    11221 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
    11281 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
    11341 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
    11401 ccgcctggaa ttcaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
    11461 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
    11521 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
    11581 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
    11641 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
    11701 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
    11761 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
    11821 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
    11881 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
    11941 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
    12001 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
    12061 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
    12121 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
    12181 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
    12241 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
    12301 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
    12361 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
    12421 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
    12481 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
    12541 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc
    12601 ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
    12661 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct
    12721 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctgcctaggg
    12781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    12841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    12901 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
    12961 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    13021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    13081 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
    13141 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    13201 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    13261 tttgcgtcga gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
    13321 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
    13381 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
    13441 ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag
    13501 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
    13561 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    13621 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    13681 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    13741 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    13801 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    13861 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
    13921 gtttacaatt tcc
//
