LOCUS Exported 11281 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway destination clone of BRSK1 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics . ACCESSION . VERSION . KEYWORDS p663-UBC-V5-BRSK1_IDG-K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11281) TITLE Major lab plasmids for proteomics-based protein-interaction and protein-proximity network generation REFERENCE 2 (bases 1 to 11281) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11281 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(350..369) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1652..1671 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1905..1922 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2118..2140 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2137..2153 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2172..2192 /label=T3 /note="T3 promoter, forward primer" promoter 2174..2192 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 2448..2628 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2672..2797 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3290..3523 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3707..3751 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4018..4134 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 4168..4188 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 4188..5399 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 5236..5255 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" protein_bind complement(5403..5423) /gene="mutant version of attB" /label=attB5 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" LTR 5427..5695 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" CDS 5705..5746 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" protein_bind 5744..5768 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(8109..8133) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 8163..8751 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8216..8236) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8634..8645) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 8765..9264 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(8800..8823) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 9197..9216 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 9285..9884 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(9285..9304) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9781..9801 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 10022..10255 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 10327..10448 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10364..10383) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10418..10437 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 10467..10602 /label=SV40 ori /note="SV40 origin of replication" primer_bind 10529..10548 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(10634..10653) /label=T7 /note="T7 promoter, forward primer" promoter complement(10635..10653) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(10663..10680) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(10663..10679) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10672..10694) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 10824..11279 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(10911..10930) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 11121..11142 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt 4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 4081 aacagacata caaactaaag aattacaaaa caaattacaa aaattcaaaa ttttatcgtc 4141 cagtgtggtg gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg 4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga 4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc 4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg 4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct 4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga 4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt 4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc 4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt 4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg 4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca 4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga 4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg 4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg 4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg 5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc 5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg 5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct 5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt 5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc 5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg 5401 gccaactttt gtatacaaag tggcgagggg ctcgcatctc tccttcacgc gcccgccgcc 5461 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg 5521 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt 5581 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc 5641 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatcggcac 5701 catgggtaag cctatcccta accctctcct cggtctcgat tctacaactt tgtacaaaaa 5761 agcaggctta atgtcgtccg gggccaagga gggaggtggg ggctctcccg cctaccacct 5821 cccccacccc cacccccacc caccccagca cgcccaatat gtgggcccct atcggctgga 5881 gaagacgctg ggcaaaggac agacagggct ggttaaactc ggggtccact gcatcacggg 5941 tcagaaggtc gccatcaaga tcgtgaaccg ggagaagctg tcggagtcgg tgctgatgaa 6001 ggtggagcgg gagatcgcca tcctgaagct catcgaacac ccacatgtcc tcaagctcca 6061 cgacgtctac gagaacaaga aatatttgta cctggttctg gagcacgtct cggggggtga 6121 gctattcgac tacctggtaa agaaggggag actgacgccc aaggaggccc gaaagttctt 6181 ccgccagatt gtgtctgcgc tggacttctg ccacagctac tccatctgcc acagagacct 6241 aaagcccgag aacctgcttt tggatgagaa aaacaacatc cgcattgcag acttcggcat 6301 ggcgtccctg caggtggggg acagcctcct ggagaccagc tgcgggtccc cccattatgc 6361 gtgtccagag gtgattaagg gggaaaaata tgatggccgc cgggcagaca tgtggagctg 6421 tggagtcatc ctcttcgccc tgctcgtggg ggctctgccc tttgatgacg acaacctccg 6481 ccagctgctg gagaaggtga aacggggcgt cttccacatg ccccacttca ttcctccaga 6541 ttgccagagc ctcctgaggg gaatgatcga agtggagccc gaaaaaaggc tcagtctgga 6601 gcaaattcag aaacatcctt ggtacctagg cgggaaacac gagccagacc cgtgcctgga 6661 gccagcccct ggccgccggg tagccatgcg gagcctgcca tccaacggag agctggaccc 6721 cgacgtccta gagagcatgg catcactggg ctgcttcagg gaccgcgaga ggctgcatcg 6781 cgagctgcgc agtgaggagg agaaccaaga aaagatgata tattatctgc ttttggatcg 6841 gaaggagcgg tatcccagct gtgaggacca ggacctgcct ccccggaatg atgttgaccc 6901 cccccggaag cgtgtggatt ctcccatgct gagccgtcac gggaagcggc gaccagagcg 6961 gaagtccatg gaagtcctga gcatcaccga tgccgggggt ggtggctccc ctgtacccac 7021 ccgacgggcc ttggagatgg cccagcacag ccagagatcc cgtagcgtca gtggagcctc 7081 cacgggtctg tcctccagcc ctctaagcag cccaaggagt ccggtctttt ccttttcacc 7141 ggagccgggg gctggagatg aggctcgagg cgggggctcc ccgacttcca aaacgcagac 7201 gctgccttct cggggcccca ggggtggggg cgccggggag cagcccccgc cccccagtgc 7261 ccgctccaca cccctgcccg gccccccagg ctccccgcgc tcctctggcg ggaccccctt 7321 gcactcgcct ctgcacacgc cccgggccag tcccaccggg accccgggga caacaccacc 7381 ccccagcccc ggcggtggcg tcgggggagc cgcctggagg agtcgtctca actccatccg 7441 caacagcttc ctgggctccc ctcgctttca ccggcgcaag atgcaggtcc ctaccgctga 7501 ggagatgtcc agcttgacgc cagagtcctc cccggagctg gcaaaacgct cctggttcgg 7561 gaacttcatc tccttggaca aagaagaaca aatattcctc gtgctaaagg acaaacctct 7621 cagcagcatc aaagcagaca tcgtccatgc ctttctgtcg atccccagcc tgagtcacag 7681 tgtgctgtca cagaccagct tcagggccga gtacaaggcc agtggcggcc cctccgtctt 7741 ccaaaagccc gtccgcttcc aggtggacat cagctcctct gagggtccag agccctcccc 7801 gcgacgggac ggcagcggag gtggtggcat ctactccgtc accttcactc tcatctcggg 7861 tcccagccgt cggttcaagc gagtggtgga gaccatccag gcacagctcc tgagcactca 7921 tgaccagccc tccgtgcagg ccctggcaga cgagaagaac ggggcccaga cccggcctgc 7981 tggtgcccca ccccgaagcc tgcagccccc acccggccgc ccagacccag agctgagcag 8041 ctctccccgc cgaggccccc ccaaggacaa gaagctcctg gccaccaacg ggacccctct 8101 gccctgaaac ccagctttct tgtacaaagt tgtgatatcc agcacagtgg cggccgctcg 8161 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 8221 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 8281 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 8341 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 8401 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 8461 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 8521 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 8581 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 8641 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 8701 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc 8761 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg 8821 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 8881 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta 8941 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta 9001 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc 9061 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga 9121 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg 9181 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc 9241 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc 9301 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 9361 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 9421 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 9481 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 9541 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 9601 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 9661 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 9721 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 9781 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 9841 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc 9901 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta 9961 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 10021 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 10081 ctctggttag accagatctg agcctgggag ctctctggct gcctagggaa cccactgctt 10141 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 10201 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 10261 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 10321 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 10381 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 10441 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 10501 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 10561 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga 10621 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 10681 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 10741 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 10801 cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 10861 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 10921 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 10981 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 11041 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 11101 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 11161 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 11221 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc 11281 c //