LOCUS Exported 1216 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION Gateway destination clone of BRSK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics . ACCESSION . VERSION . KEYWORDS p663-UBC-miniTurbo-V5-BRSK2_IDG-K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1216) TITLE Major lab plasmids for proteomics-based protein-interaction and protein-proximity network generation REFERENCE 2 (bases 1 to 1216) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1216 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(139..159) /gene="mutant version of attB" /label=attB5 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" regulatory 160..169 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 172..942 /codon_start=1 /product="N-terminally truncated mutant of the E. coli biotin ligase BioID engineered for improved catalytic efficiency (Branon et al., 2018)" /label=miniTurbo /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF GISRGIDKHGALLLEQDGVIKPWMGGEISLRSAEK" CDS 949..990 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" ORIGIN 1 actggctgcg agtgtgtttt gtgagttttt taggcacctt ttgaaatgta atcatttggg 61 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg 121 gcttttttgt tagacggcca acttttgtat acaaagtggg ccaccatggg aatcccgctg 181 ctgaacgcta aacagattct gggacagctg gacggcggga gcgtggcagt cctgcctgtg 241 gtcgactcca ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct 301 tgcattgcag aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct 361 tttggagcta acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc 421 ggcctgggcc cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac 481 aaggtgcgag tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc 541 ctggtggagc tggccggaat aacaggcgat gctgcacaga tcgtcattgg cgccgggatt 601 aacgtggcta tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat cacactgcag 661 gaagcaggga ttaacctgga caggaatact ctggccgcta tgctgatccg agagctgcgg 721 gcagccctgg aactgttcga gcaggaaggc ctggctccat atctgtcacg gtgggagaag 781 ctggataact tcatcaatag acccgtgaag ctgatcattg gggacaaaga gattttcggg 841 attagccggg ggattgataa acacggagcc ctgctgctgg aacaggacgg agttatcaaa 901 ccctggatgg gcggagaaat cagtctgcgg tctgccgaaa aggctagcgg caagcccatc 961 cccaaccccc tgctgggcct ggacagcaca caactttgta caaaaagcag atggcccgtc 1021 aaggcccacc atgacatcga cgggaagacg gcgcgcgcag cacgcgcagt atgttgggcc 1081 ctacgcctga gaagacgctg ggcagggcag acagtctgtg agctggggtc actgcgtcac 1141 ttgcaaagtg ncatcagatc gtcaacgtgg aaaagcttca ccgagtcggt gcctgattga 1201 aggtgggagc gggnat //