LOCUS       Exported                1171 bp ds-DNA     linear   SYN 11-JUN-2021
DEFINITION  Gateway destination clone of CDK13 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-CMV-Flag-CDK13_IDG-K
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1171)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      115..124
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             124..147
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    157..181
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggaccaggga gcaatagcag agctcgttta gtgaccgtca gatcgcctgg agacgccatc
       61 cacgctgttt tgacctccat agaagacacc ggcggccata ccggtcagct cgaggccacc
      121 atggactaca aggacgacga cgacaagggc ggatcaacaa gtttgtacaa aaaagcagaa
      181 gggccgtcaa ggcccaccat gccgagcagc tcggacacgg cgctgggggg aggcgggggc
      241 ctgagctggg cggagaagaa gttggaggaa cgccgcaagc ggaggcgatt cctgtcccct
      301 cagcagccgc cgctgctgtt gccgctcctg cagccgcagc tcctgcaacc gccgccgccc
      361 ccgccgcctc tgctcttcct ggctgctccc ggcacggccg ccgccgcagc cgccgccgcc
      421 gcggcctcct cctcttgctt cagcccgggc ccccctctgg aggtcaagcg gctggcgaga
      481 ggcaagaggc gcgcaggagg gcggcagaag cggcgtcgcg ggccccgcgc cgggcaggag
      541 gcggagaagc gtcgggtctt ctcgctgccc cagccgcagc aggacggcgg tggcggtgct
      601 agtagcggcg ggggtgtgac cccgctggtg gaatacgagg atgtgagctc ccagtccgag
      661 caggggctgc tgctgggggg ggccagcgcg gcaacggcgg cgacggctgc cgggggaacg
      721 gggggcagcg gcgggagtcc ggcctcctcc tccggcaccc agcggcgcgg ggaggggtcg
      781 gagcgcagcc ccgccgggac cgccgcagca gcagtggccg cagcaaggag cgccaccgcg
      841 agcaccggcg gcgggatggg cagcgcggtg gcagcgaggc ctccaagtcc cgcagccgcc
      901 acagccacag cggcgaggaa cgggccgagg tcgcccagag cggcagcagc agcagcagcg
      961 gcggccgccg gaaaagcgct tcggccacat ccagcagcag tagcagccgc aaggaccggg
     1021 actcgaaggc ccaccgcagc cggactagtc gtccagagcc gcttcggcta cagaccgcca
     1081 gcctaccggg aggacagacg agctagctan gcgcgcgtcc ctcagccact gggaggcggg
     1141 acgacgccgg tgttccacag gcctcttnan c
//
