LOCUS       Exported               10608 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Myosin-IIA-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Jacobelli J, Bennett FC, Pandurangi P, Tooley AJ, Krummel MF
  TITLE     Myosin-IIA and ICAM-1 regulate the interchange between two distinct 
            modes of T cell migration.
  JOURNAL   J Immunol. 2009 Feb 15;182(4):2041-50.
  PUBMED    19201857
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      243..252
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             249..965
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(294..315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(555..574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     902..923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7032..7153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7069..7088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7123..7142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7160..7615)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7297..7318)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7509..7528
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7642..7746
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7748..8105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(7771..7791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7956..8091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8018..8037
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8140..8934
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8194..8213)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8804..8823
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9122..9141)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9166..9213
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9542..10130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10031..10050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        10305..10608
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      301 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      361 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      421 ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc
      481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      541 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      601 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      661 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      721 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      841 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      901 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      961 acaagtccgg tgcctcgagc gccaccctgg ctatctccgg aggtggcggg agcggaatcc
     1021 tgatggctca gcaggctgca gacaagtacc tctatgtgga taaaaacttc atcaataacc
     1081 cgctggccca agctgactgg gctgccaaga agttggtatg ggtgccttcc agcaagaatg
     1141 gctttgaacc agctagcctc aaggaggagg tgggagaaga ggccattgta gagctggtag
     1201 agaatgggaa gaaggtgaag gtgaacaagg acgacatcca gaagatgaac ccacccaagt
     1261 tctccaaggt ggaggacatg gcagagctca cgtgcctcaa cgaagcttcg gtgctgcaca
     1321 acctcaagga gcgatactac tcagggctta tctacaccta ttcaggcctg ttctgtgtgg
     1381 tcatcaaccc ttataagaac ctgcccatct actcagagga gatcgtggag atgtacaagg
     1441 gcaagaagag gcacgagatg ccaccccaca tctacgccat cacagatact gcctaccgga
     1501 gcatgatgca ggaccgggaa gatcagtcca tcctgtgcac gggggagtct ggagcaggga
     1561 agacagagaa caccaagaaa gtcatccagt acctggcaca tgtggcctcc tcacacaaga
     1621 gcaagaagga ccagggggag ttggagcggc agctgctaca ggccaaccct atcctagagg
     1681 cctttggaaa cgccaagacg gtgaagaatg acaactcctc tcgattcggt aaattcattc
     1741 gtatcaactt tgatgtcaat ggctacattg ttggtgccaa cattgagact tatcttctgg
     1801 agaaatctcg tgctatccgc caagccaaag aggagcggac cttccacatc ttctactacc
     1861 tgctgtctgg ggccggagaa cacctgaaga ctgatctcct gttggagcca tacaacaaat
     1921 accgcttcct gtccaacggg cacgtcacca tccctgggca gcaggacaag gacatgttcc
     1981 aggagacaat ggaggccatg agaattatgg gcatcccaga ggatgagcag atgggcttgc
     2041 tgcgggtcat ctctggggtc cttcagcttg gcaacattgc cttcaagaag gagcggaaca
     2101 ctgaccaggc gtccatgccg gacaacacag ctgctcaaaa ggtgtcccac ctcctgggga
     2161 tcaatgtgac cgacttcacc agaggcatcc tcaccccacg catcaaggtg ggcagagact
     2221 atgtgcagaa ggcgcagact aaagagcagg ctgactttgc cattgaggcc ttggccaagg
     2281 ctacctatga gcggatgttc cgctggctgg tgcttcgcat caacaaagct ctggacaaga
     2341 ccaagaggca gggcgcctca tttatcggga tcctggacat cgctggcttt gagatctttg
     2401 atctgaactc cttcgagcag ctgtgcatca actacaccaa cgagaagctg cagcagctgt
     2461 tcaaccacac catgttcatc ctggagcagg aggagtacca gcgagagggc atcgagtgga
     2521 acttcatcga cttcggcctg gacctgcagc cctgcatcga cctcattgag aagccggcgg
     2581 gtcccccagg catcctggcc ctgctagatg aggagtgctg gtttcctaag gccactgaca
     2641 agagcttcgt ggagaaggtg gtgcaggagc agggcaccca ccccaagttc cagaagccca
     2701 agcaactgaa ggacaaggct gatttctgca ttatccacta tgccggcaag gtggactata
     2761 aagctgacga gtggctgatg aagaacatgg accccttgaa cgacaacatc gccacgctgc
     2821 ttcaccagtc ctcagacaag tttgtctctg agctgtggaa ggatgtggat cggatcattg
     2881 gcttggacca agtggctgga atgtccgaga cagcactacc tggtgccttc aagacccgga
     2941 agggcatgtt ccgtactgtc ggacagctgt acaaggagca gctggccaag ctcatggcca
     3001 cgttgaggaa taccaacccc aacttcgtgc gctgcatcat tcccaaccat gagaagaagg
     3061 ccggcaaact ggacccgcac ttggtgctgg accagctgcg ctgcaatggc gtccttgagg
     3121 gcatccggat ctgccgccag ggctttccca acagggtggt cttccaggag ttccggcaga
     3181 ggtatgagat cctcaccccc aactccatcc cgaagggctt catggatggc aagcaagcgt
     3241 gtgtgctcat gatcaaagcc ttggagcttg acagcaacct gtaccgcatc ggccagagca
     3301 aagtgttctt ccgggcagga gtgctagccc acctggagga agagcgggac ctgaagatca
     3361 ccgatgtcat cattggcttc caggcctgct gcaggggcta cctggccagg aaggcctttg
     3421 ccaagaggca gcaacagctg accgccatga aggtcctaca gaggaactgt gctgcgtacc
     3481 tcaggctgcg caactggcag tggtggaggc tcttcaccaa ggtcaagccc ctgttgaact
     3541 caataagaca tgaggatgag ctgttagcca aggaggcgga actgacaaag gttcgagaga
     3601 aacatctggc tgcagagaac aggctgacag agatggagac gatgcagtct cagctcatgg
     3661 cagagaagct gcagcttcag gagcagctgc aggcggagac agagctgtgt gccgaggctg
     3721 aggagctccg ggcccgtctg acagcgaaga agcaggagct ggaggagatc tgccatgacc
     3781 tggaggccag ggtggaggag gaggaggagc gctgccagta cctgcaggcc gagaagaaga
     3841 agatgcagca gaacatccag gaacttgagg agcagttgga ggaggaggag agcgcccggc
     3901 agaagctgca gcttgagaag gtgaccaccg aggccaagct gaagaaactg gaggaggacc
     3961 agatcatcat ggaggaccag aactgcaaac tggccaagga gaagaaactg ctggaagaca
     4021 gagtagctga attcactacc aacctcatgg aagaggagga gaagtccaag agcctggcca
     4081 agctcaagaa caagcacgag gcaatgatca ccgacctgga agagcgcctc cgtagggagg
     4141 agaagcagag gcaggagttg gagaagaccc gtcgcaagct ggagggagac tccacagacc
     4201 tcagtgacca gattgctgag ctccaggcgc agatagcaga gctcaagatg cagctggcca
     4261 agaaggagga ggagttgcag gctgccttgg ccagagtgga agaagaagct gctcagaaga
     4321 atatggccct gaagaagatc cgagaactgg aaactcagat ctctgagctc caggaggacc
     4381 tggagtcgga gcgagcctcc aggaataaag ccgagaagca gaaacgggat ctgggagagg
     4441 agctggaggc gctgaagaca gagctggagg acacgctgga ctccacggct gcccagcagg
     4501 agctgaggtc gaagcgtgag caggaggtga gcatcctgaa gaagactctg gaggacgagg
     4561 ccaagaccca tgaggcccag atccaggaga tgaggcagaa gcactcacag gctgtggagg
     4621 agctggcaga tcagttggag cagacgaagc gggtaaaagc tacccttgag aaggcgaagc
     4681 agaccctgga gaatgagcgg ggagagctgg ccaatgaggt gaaggccctg ctgcaaggca
     4741 agggcgactc agagcacaag cgcaagaagg tggaggcgca gctgcaagaa ctgcaggtca
     4801 agttcagcga gggagagcgc gtgcgaaccg aactggccga caaggtcacc aagctgcagg
     4861 ttgaactgga cagtgtgacc ggtctcctta gccagtctga cagcaagtcc agcaagctta
     4921 cgaaggactt ctctgcgctg gagtcccagc ttcaggacac acaggagttg ctccaggagg
     4981 agaaccggca gaagctgagc ctgagcacca agctcaagca gatggaggat gagaaaaact
     5041 ccttcaggga gcagctggag gaggaggagg aggccaagcg caacttggag aagcagatcg
     5101 ccacgctcca tgcccaggtg accgacatga agaagaagat ggaggacggt gtagggtgcc
     5161 tggagactgc agaggaggcg aagcggaggc ttcagaagga cttggaaggc ctgagccagc
     5221 ggcttgagga gaaggtggct gcctacgata agctggagaa gaccaagaca cggctgcagc
     5281 aggagctgga cgacctgctg gttgacctgg accaccagcg gcagagcgtc tccaacctgg
     5341 aaaagaagca gaagaagttc gaccagctcc tagccgagga gaagaccatc tcggccaagt
     5401 atgcagagga gcgtgaccga gctgaggctg aggcccgtga gaaggagaca aaggcgctat
     5461 cactggcccg ggcgcttgag gaggccatgg agcagaaggc agagctggag cggctcaaca
     5521 agcagttccg cacggagatg gaggacctca tgagctccaa ggatgacgtg ggcaagagtg
     5581 tccacgagct ggagaagtcc aagcgggcct tggagcagca ggtggaggag atgaagaccc
     5641 agctggagga gctggaggat gagctgcagg ccacggagga tgccaagctc cgcctggagg
     5701 tgaacctgca ggccatgaag gcccagtttg agcgggatct gcagggccgg gatgaacaga
     5761 gcgaggagaa gaagaagcag ctggtcagac aggtgcggga gatggaggcg gagctggagg
     5821 atgagaggaa gcagcgctcc atggccatgg ccgcacgcaa gaaactggag atggatctga
     5881 aggacctgga ggcacacatt gacacagcca ataagaaccg ggaagaggcc atcaaacagc
     5941 tgcggaagct tcaggcccag atgaaggact gcatgcggga gctggacgac acgcgcgcct
     6001 cccgggagga gatcctggcg caggccaagg agaatgagaa gaagctgaag agcatggagg
     6061 ccgagatgat tcagctgcag gaggaactgg cagctgctga gcgtgctaag cgtcaggccc
     6121 aacaggaacg ggacgagctg gctgatgaga tcgccaacag cagtggcaaa ggggccctag
     6181 cattagagga gaagcggcga ctggaggccc gcattgccca gctggaggag gagctggagg
     6241 aggaacaggg caacacggag ctgatcaacg atcggctgaa gaaggccaac ctgcagatcg
     6301 accaaataaa caccgacctg aacctggaac gcagccacgc acagaagaat gagaatgcgc
     6361 gacagcagct ggaacgccag aacaaggagc tcaaggccaa gctgcaggaa atggagagtg
     6421 ctgtcaagtc caaatacaag gcctccatcg cggccttgga ggccaaaatt gcacagctgg
     6481 aggaacagct ggacaacgag accaaggagc gccaggcagc ctccaagcag gtgcgccgga
     6541 cggagaagaa gctgaaggac gtgctgctgc aggtggagga cgagcggagg aacgcggaac
     6601 agttcaagga ccaggctgac aaggcgtcca cccgcctgaa gcagcttaaa cggcagctag
     6661 aggaggctga agaggaggcc cagcgggcca atgcctcacg ccggaagctg cagcgtgagc
     6721 tggaagatgc cacagagacc gctgatgcta tgaaccgcga ggtcagctcc ctgaagaaca
     6781 aactgaggcg tggggacctg ccatttgtcg tgactcgccg aattgttcgg aaaggcactg
     6841 gcgactgctc agacgaggag gtcgacggta aagcagatgg ggccgatgcc aaggcagctg
     6901 aataggcggc cgcttctaga taactgatca taatcagcca taccacattt gtagaggttt
     6961 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     7021 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7081 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7141 tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     7201 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     7261 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     7321 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     7381 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     7441 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     7501 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     7561 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     7621 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7681 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7741 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     7801 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7861 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7921 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7981 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8041 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8101 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     8161 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     8221 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     8281 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     8341 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     8401 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     8461 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     8521 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     8581 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     8641 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     8701 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     8761 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     8821 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     8881 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     8941 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     9001 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     9061 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     9121 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     9181 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     9241 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     9301 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     9361 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     9421 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9661 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10201 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa
    10261 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
    10321 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
    10381 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
    10441 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
    10501 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
    10561 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatg
//
