LOCUS Exported 820 bp ds-DNA linear SYN 15-JUN-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS Vh-HA-2xFRB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 820) AUTHORS Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, Cornish VW, Bertozzi CR TITLE Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. JOURNAL J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. PUBMED 18788807 REFERENCE 2 (bases 1 to 820) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 15, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..820 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(94..372) /codon_start=1 /product="FKBP-rapamycin binding domain of human FRAP" /label=FRB /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK" CDS complement(379..657) /codon_start=1 /product="T2098L mutant of FKBP-rapamycin binding domain of human FRAP" /label=FRB* /note="binds synthetic ligands such as AP21967" /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK" CDS complement(658..684) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" ORIGIN 1 acgggcctct agactcgagc ggccgccact gtgctggata tctgcagaat tccaccacac 61 tggactagtg gatccgagct cggtacctct agtctttgag attcgtcgga acacatgata 121 atagaggtcc caggcttggg tgaggtcctt gacattccct gatttcatgt acttcctgca 181 ccactcttgg gcctccatta aatctcgacc ataggcctga ttaaaggatg tttccttcag 241 agtctggggg ccccgttcca tcatagcatg caagggctcc agcacctcaa acatgccttt 301 cacgttcctt tccccaaagt acaaacgaga tgcctcttcc aggccttcat gccacatctc 361 atgccagagg attctagtct ttgagattcg tcggaacaca tgataataga ggtcccaggc 421 ttggaggagg tccttgacat tccctgattt catgtacttc ctgcaccact cttgggcctc 481 cattaaatct cgaccatagg cctgattaaa ggatgtttcc ttcagagtct gggggccccg 541 ttccatcata gcatgcaagg gctccagcac ctcaaacatg cctttcacgt tcctttcccc 601 aaagtacaaa cgagatgcct cttccaggcc ttcatgccac atctcatgcc agaggattgc 661 gtagtctggt acgtcgtacg gatatctaga ctcgaggtcg acctgcagtt ggacctggga 721 gtgcgcgcct gtggagagaa aggcaaagtg gatgtcattg tcacccatat atatgtccag 781 acctcaagcc tgctactgtt aaccccttac ctgtagctgt //