LOCUS Exported 3930 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC39A8 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3930) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 3930) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3930 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2055..2154) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2171..2190) /label=T7 /note="T7 promoter, forward primer" promoter complement(2172..2190) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2195..2211) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2195..2211) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2313..2332) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2324..3133 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2391..2410) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3280..3868 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3769..3788 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggccc ctggccgggc cgtggccggc ctgctgctgc tggcagcagc 721 aggactggga ggagtggccg agggcccagg cctggccttt tccgaggacg tgctgagcgt 781 gttcggagca aacctgtccc tgtctgccgc acagctgcag cacctgctgg agcagatggg 841 agcagcatcc agagtgggag tgccagagcc tggccagctg cacttcaacc agtgtctgac 901 cgccgaggag atctttagcc tgcacggctt ctccaatgcc acccagatca caagctccaa 961 gttttctgtg atctgccccg ccgtgctgca gcagctgaac ttccacccgt gcgaggatag 1021 gccgaaacat aagacaagac ctagccactc cgaagtgtgg ggctacggct ttctgtctgt 1081 gaccatcatc aatctggcca gcctgctggg cctgatcctg acaccactga tcaagaagtc 1141 ctatttcccc aagatcctga ccttctttgt gggcctggcc atcggcacac tgttttctaa 1201 cgccatcttc cagctgatcc ccgaggcctt tggcttcgac cctaaggtgg atagctacgt 1261 ggagaaggcc gtggccgtgt ttggcggctt ctatctgctg ttctttttcg agaggatgct 1321 gaagatgctg ctgaagacct acggccagaa tggccacaca cactttggca acgacaattt 1381 cggcccccag gagaagaccc accagccaaa ggccctgcca gccatcaacg gagtgacctg 1441 ctatgccaat cctgccgtga cagaggccaa cggccacatc cactttgaca acgtgagcgt 1501 ggtgtctctg caggatggca agaaggagcc ttctagctgc acctgtctga agggcccaaa 1561 gctgtccgag atcggcacca tcgcctggat gatcacactg tgcgacgccc tgcacaactt 1621 catcgatggc ctggccatcg gcgcctcttg taccctgagc ctgctgcagg gcctgtctac 1681 aagcatcgcc atcctgtgcg aggagtttcc ccacgagctg ggcgacttcg tgatcctgct 1741 gaacgccggc atgtccacca gacaggccct gctgtttaat ttcctgagcg cctgctcctg 1801 ttacgtggga ctggccttcg gcatcctggt gggcaacaat tttgccccaa atatcatctt 1861 cgccctggcc ggcggcatgt ttctgtatat cagcctggcc gacatgttcc ccgagatgaa 1921 cgatatgctg agggagaagg tgaccggccg caagaccgac ttcaccttct tcatgatcca 1981 gaatgccggc atgctgaccg gcttcacagc catcctgctg atcacactgt acgccggcga 2041 gatcgagctg gaggacccag ctttcttgta caaagttggc attataagaa agcattgctt 2101 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc 2161 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg 2221 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac 2281 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg 2341 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg 2401 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga 2461 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga 2521 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat 2581 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca 2641 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct 2701 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg 2761 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga 2821 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt 2881 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga 2941 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga 3001 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt 3061 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga 3121 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac 3181 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca 3241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 3301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 3361 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 3421 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 3481 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 3541 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 3601 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 3661 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 3721 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 3781 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 3841 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 3901 ggccttttgc tggccttttg ctcacatgtt //