LOCUS Exported 4356 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC6A13 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4356) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4356) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4356 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2481..2580) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2597..2616) /label=T7 /note="T7 promoter, forward primer" promoter complement(2598..2616) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2621..2637) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2621..2637) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2739..2758) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2750..3559 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2817..2836) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3706..4294 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4195..4214 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggact cccgggtgtc tggcaccaca agcaacggcg agacaaagcc 721 agtgtacccc gtgatggaga agaaggagga ggatggcacc ctggagagag gccactggaa 781 caataagatg gagttcgtgc tgtccgtggc aggagagatc atcggactgg gcaacgtgtg 841 gaggtttcca tacctgtgct ataagaatgg cggcggcgcc ttctttatcc cctacctggt 901 gtttctgttc acctgcggca tccccgtgtt cctgctggag accgccctgg gccagtatac 961 atcccaggga ggagtgaccg catggaggaa gatctgtccc atcttcgagg gcatcggcta 1021 cgcctctcag atgatcgtga tcctgctgaa cgtgtactat atcatcgtgc tggcctgggc 1081 cctgttttac ctgttcagct cctttaccat cgacctgccc tggggcggct gttatcacga 1141 gtggaacaca gagcactgca tggagtttca gaagaccaac ggctccctga atggcacctc 1201 tgagaatgcc acaagccctg tgatcgagtt ctgggagcgg agagtgctga agatctctga 1261 tggaatccag cacctgggcg ccctgagatg ggagctggcc ctgtgcctgc tgctggcctg 1321 ggtcatctgc tacttttgta tctggaaggg cgtgaagagc acaggcaagg tggtgtactt 1381 caccgccaca tttccttatc tgatgctggt ggtgctgctg atcaggggag tgaccctgcc 1441 aggagcagca cagggcatcc agttttacct gtatcccaac ctgacacggc tgtgggaccc 1501 tcaagtgtgg atggatgccg gcacccagat cttcttttct ttcgccatct gcctgggctg 1561 tctgacagcc ctgggcagct acaacaagta tcacaacaat tgctatagag actgtatcgc 1621 cctgtgcttc ctgaatagcg gcacctcctt tgtggccggc ttcgccatct ttagcatcct 1681 gggcttcatg tcccaggagc agggcgtgcc tatctccgag gtggcagagt ctggaccagg 1741 actggccttc atcgcatacc caagagccgt ggtcatgctg cctttttccc cactgtgggc 1801 ctgctgtttc tttttcatgg tggtgctgct gggcctggac tctcagttcg tgtgcgtgga 1861 gagcctggtg acagccctgg tggatatgta tccccacgtg tttaggaaga agaataggcg 1921 cgaggtgctg atcctgggcg tgtctgtggt gagcttcctg gtgggcctga tcatgctgac 1981 cgagggcggc atgtacgtgt tccagctgtt tgattactat gccgccagcg gcatgtgcct 2041 gctgttcgtg gccatctttg agtccctgtg cgtggcatgg gtgtacggag caaagcggtt 2101 ctacgacaac atcgaggata tgatcggcta caggccctgg cctctgatca agtattgttg 2161 gctgttcctg acacccgccg tgtgcaccgc cacatttctg ttctccctga tcaagtacac 2221 ccctctgaca tataataaga agtacaccta tccatggtgg ggcgacgcac tgggatggct 2281 gctggccctg tctagcatgg tgtgcatccc agcatggagc ctgtacaggc tgggcacact 2341 gaagggaccc ttccgggaga gaatcaggca gctgatgtgc cctgccgagg atctgccaca 2401 gaggaaccca gcaggacctt ccgccccagc aaccccacgc acatctctgc tgaggctgac 2461 cgagctggag agccactgcg acccagcttt cttgtacaaa gttggcatta taagaaagca 2521 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg 2581 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca 2641 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc 2701 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat 2761 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta 2821 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa 2881 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac 2941 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca 3001 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc 3061 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt 3121 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt 3181 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt 3241 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt 3301 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt 3361 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata 3421 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg 3481 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat 3541 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac 3601 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc 3661 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 3721 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 3781 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 3841 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 3901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 3961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 4021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 4081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 4141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 4201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 4261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 4321 gttcctggcc ttttgctggc cttttgctca catgtt //